User:Andy Maloney/Notebook/Lab Notebook of Andy Maloney/2010/03/31/Fun with dynamic page listings
From OpenWetWare
I think I have finally gotten something to report for dynamic page listings. It appears that you can setup a template and in the template, write a description for the page. This description can be used as a summary for a dynamic page listing. The code for the dpl is below.
{{#dpl: |namespace=User |order=descending |addcategories=true |uses=Template:AndyMaloneyNotebook |includepage={AndyMaloneyNotebook}:[[%PAGE%]] |includepage={AndyMaloneyNotebook}:Description |format=,\n*[[%PAGE%]]\n**'''Categories:''' %CATLIST%\n**'''Description:''' ,, }}
- namespace=User
- This just tells dpl to start looking in the special page Users where all our notebooks belong.
- order=descending
- I'm pretty sure that this means that the pages shown will show up in dpl in a descending order. I think that the first page shown is not the most recent edited page but the most recent created page.
- addcategories=true
- This just allows me to add a list of categories used on the page. Since I am using a user specified format for the output of dpl, I have to include this command as well as the one below in the format.
- uses=Template:AndyMaloneyNotebook
- This tells dpl that the pages it should list, must have the template AndyMaloneyNotebook. Otherwise, it won't include it.
- includepage=
- So there are two things that I want to include in the list namely, the page name and a description. I have to break up this into two lines (hence the two includepage commands) in order for dpl to format the output in the way I want.
- format=
- So you must start this line after the = sign with one comma. You must end it in 2 commas. dpl will allow wiki markup as you can see with the stars and apostrophes dictating a list and bold type. The \n is required for dpl to start a new line.
Output
- User:Andy Maloney/Notebook/Lab Notebook of Andy Maloney/2009/02/19/Sketchup
- Categories: AM Engineering, Sketchup
- Description: This page talks about my Google warehouse where I have uploaded all my Sketchup models of optomechanics. The link to the ware house is here.
- User:Andy Maloney/Notebook/Lab Notebook of Andy Maloney/2009/02/19/Optical tweezers
- Categories: AM Engineering, Diode laser, Optical tweezers, Sample translation stage
- Description: Brainstorming the parts needed for an optical tweezers.
- User:Andy Maloney/Notebook/Lab Notebook of Andy Maloney/2009/02/20/Optical tweezers
- Categories: Optical tweezers
- Description: More brainstorming on the optical tweezers.
- User:Andy Maloney/Notebook/Lab Notebook of Andy Maloney/2009/02/22/Optical tweezers
- Categories: AM Engineering, Beam shaping, Diode laser, Optical tweezers, Sample translation stage, Spatial filtering
- Description: Thoughts on beam shaping and spatial filtering. More talk of the optical tweezers.
- User:Andy Maloney/Notebook/Lab Notebook of Andy Maloney/2009/02/23/Optical tweezers
- Categories: AM Engineering, Beam shaping, Condenser detector stage, Diode laser, Optical tweezers, Sample translation stage, Spatial filtering
- Description: Moving on to thinking about the condenser and detector stage.Thoughts on beam shaping and spatial filtering. More talk of the optical tweezers.
- User:Andy Maloney/Notebook/Lab Notebook of Andy Maloney/2009/02/24/Wetlab
- Categories: AM Engineering, Wet lab
- Description: Brainstorming the wetlab install.
- User:Andy Maloney/Notebook/Lab Notebook of Andy Maloney/2009/02/25/Optical tweezers
- Categories: AM Engineering, Optical tweezers
- Description: Best surplus optics supplier around!
- User:Andy Maloney/Notebook/Lab Notebook of Andy Maloney/2009/03/06/Optical Tweezers
- Categories: AM Engineering, Condenser detector stage, Optical tweezers
- Description: Lamenting about the condenser adapter that fits into Thorlabs' 60mm cage cube.
- User:Andy Maloney/Notebook/Lab Notebook of Andy Maloney/2009/03/09/Optical tweezer
- Categories: AM Engineering, Condenser detector stage, Optical tweezers
- Description: More lamenting on the condenser adapter.
- User:Andy Maloney/Notebook/Lab Notebook of Andy Maloney/2009/03/10/Optical tweezers
- Categories: AM Engineering, Condenser detector stage, Diode laser, Optical tweezers
- Description: Final design for the condenser adapter and initial work with the PLT laser diode.
- User:Andy Maloney/Notebook/Lab Notebook of Andy Maloney/2009/03/11/Modeling DNA unzipping
- Categories: DNA unzipping, Modeling DNA unzipping
- Description: My first look at modeling of DNA unzipping.
- User:Andy Maloney/Notebook/Lab Notebook of Andy Maloney/2009/03/12/Kinesin & Microtubules
- Categories: Kinesin and microtubule basics, Kinesin and microtubule papers, Kinesin and microtubules
- Description: One of the first papers I reviewed. Nanomechanics of Microtubules
- User:Andy Maloney/Notebook/Lab Notebook of Andy Maloney/2009/03/17/Kinesin & Microtubules
- Categories: Kinesin and microtubule basics, Kinesin and microtubule papers, Kinesin and microtubules
- Description: Paper review. Kinesin Movement on Glutaraldehyde-Fixed Microtubules
- User:Andy Maloney/Notebook/Lab Notebook of Andy Maloney/2009/03/17/Optical tweezers
- Categories: AM Engineering, Diode laser, Optical tweezers
- Description: Amperage usage in the control box and PID adjustments.
- User:Andy Maloney/Notebook/Lab Notebook of Andy Maloney/2009/03/18/Optical tweezers
- Categories: AM Engineering, Condenser detector stage, Diode laser, Optical tweezers
- Description: Olympus dovetail mount design.
- User:Andy Maloney/Notebook/Lab Notebook of Andy Maloney/2009/03/19/Optical tweezers
- Categories: AM Engineering, Diode laser, Optical tweezers
- Description: Found out that we weren't supplying enough current to the controller thus we weren't able to keep the temperature stable.
- User:Andy Maloney/Notebook/Lab Notebook of Andy Maloney/2009/03/20/Optical tweezers
- Categories: AM Engineering, Condenser detector stage, Diode laser, Optical tweezers
- Description: Update for the dovetail machined from John DeMoss. Also, I talk about some of the output connectors on the control box we can use for monitoring the system.
- User:Andy Maloney/Notebook/Lab Notebook of Andy Maloney/2009/03/25/Optical tweezers
- Categories: AM Engineering, Diode laser, Optical tweezers
- Description: Building the laser diode control box.
- User:Andy Maloney/Notebook/Lab Notebook of Andy Maloney/2009/03/27/Optical tweezers
- Categories: AM Engineering, Diode laser, Optical tweezers
- Description: New power supply build. PID control and talk about anamorphic prisms.
- User:Andy Maloney/Notebook/Lab Notebook of Andy Maloney/2009/03/30/Optical tweezers
- Categories: Diode laser, Optical tweezers
- Description: Futile attempts at collimating the laser diode.
- User:Andy Maloney/Notebook/Lab Notebook of Andy Maloney/2009/03/31/Optical tweezers
- Categories: AM Engineering, Diode laser, Optical tweezers
- Description: More futile attempts at collimating the laser diode.
- User:Andy Maloney/Notebook/Lab Notebook of Andy Maloney/2009/04/01/Optical tweezers
- Categories: AM Engineering, Diode laser, Optical tweezers
- Description: Space issues, collimation and such.
- User:Andy Maloney/Notebook/Lab Notebook of Andy Maloney/2009/04/02/Optical tweezers
- Categories: AM Engineering, Diode laser, Optical tweezers
- Description: Futile belief that the laser is collimated.
- User:Andy Maloney/Notebook/Lab Notebook of Andy Maloney/2009/04/10/Kinesin & Microtubules
- Categories: Kinesin and microtubule basics, Kinesin and microtubule papers, Kinesin and microtubules
- Description: Paper review. Equilibrium and Transition between Single- and Double-Headed Binding of Kinesin as Revealed by Single-Molecule Mechanics
- User:Andy Maloney/Notebook/Lab Notebook of Andy Maloney/2009/04/13/Kinesin & Microtubules
- Categories: Kinesin and microtubule basics, Kinesin and microtubule papers, Kinesin and microtubules
- Description: Paper review. This is in my notebook twice. Since I'm labeling things in hindsight, I'm not sure why this is the case. Equilibrium and Transition between Single- and Double-Headed Binding of Kinesin as Revealed by Single-Molecule Mechanics
- User:Andy Maloney/Notebook/Lab Notebook of Andy Maloney/2009/04/13/Meeting with Koch
- Categories: Kochlab meetings
- Description: Going over notes from a meeting with Koch.
- User:Andy Maloney/Notebook/Lab Notebook of Andy Maloney/2009/04/15/Kinesin and µ-tubules
- Categories: Kinesin and microtubule papers, Kinesin and microtubules
- Description: Paper review. Equilibrium and Transition between Single- and Double-Headed Binding of Kinesin as Revealed by Single-Molecule Mechanics
- User:Andy Maloney/Notebook/Lab Notebook of Andy Maloney/2009/04/15/Optical tweezers
- Categories: AM Engineering, Optical tweezers, Optical tweezers calibration
- Description: Optical tweezer calibration overview and my thoughts on what it means.
- User:Andy Maloney/Notebook/Lab Notebook of Andy Maloney/2009/04/16/Kinesin & Microtubules
- Categories: Kinesin and microtubule papers, Kinesin and microtubules
- Description: Paper review. Direct observation of kinesin stepping by optical trapping interferometry
- User:Andy Maloney/Notebook/Lab Notebook of Andy Maloney/2009/04/17/Kinesin & Microtubules
- Categories: AM Engineering
- Description: Parts ordering.
- User:Andy Maloney/Notebook/Lab Notebook of Andy Maloney/2009/04/20/Microtubule paper review
- Categories: AM SurfacePassivationMTs&Kinesin, Kinesin and microtubule basics, Kinesin and microtubule papers, Kinesin and microtubules, Kochlab questions
- Description: Paper review and thoughts about casein.
- User:Andy Maloney/Notebook/Lab Notebook of Andy Maloney/2009/04/21/Kinesin & Microtubules
- Categories: Kinesin and microtubule papers, Kinesin and microtubules, Kochlab questions
- Description: Paper review. How calcium causes microtubule depolymerization
- User:Andy Maloney/Notebook/Lab Notebook of Andy Maloney/2009/04/22/Kinesin & Microtubules
- Categories: Kinesin and microtubule basics, Kinesin and microtubules
- Description: Talking about the buffers used for the gliding motility assay.
- User:Andy Maloney/Notebook/Lab Notebook of Andy Maloney/2009/04/23/The role of casein in supporting the operation of surface bound kinesin
- Categories: Kinesin and microtubule papers, Kinesin and microtubules
- Description: Paper review. The role of casein in supporting the operation of surface bound kinesin
- User:Andy Maloney/Notebook/Lab Notebook of Andy Maloney/2009/04/24/More on kinesin & microtubule buffers
- Categories: Kinesin and microtubule basics, Kinesin and microtubules, Surface passivation
- Description: Found a website talking about caseins.
- User:Andy Maloney/Notebook/Lab Notebook of Andy Maloney/2009/04/27
- Categories: AM Engineering
- Description: My first LabVIEW program.
- User:Andy Maloney/Notebook/Lab Notebook of Andy Maloney/2009/04/28/Koch Lab Kinesin Logo
- Categories: AM Engineering, Kinesin and microtubules
- Description: Birth of Kiney.
- User:Andy Maloney/Notebook/Lab Notebook of Andy Maloney/2009/04/30/Optical tweezers stuff
- Categories: AM Engineering, Diode laser, Optical tweezers
- Description: More on collimating the diode laser.
- User:Andy Maloney/Notebook/Lab Notebook of Andy Maloney/2009/05/08/Kinetic analysis of GTP hydrolysis associated with tubulin polymerization review
- Categories: Kinesin and microtubule papers, Kinesin and microtubules
- Description: Paper review. Kinetic analysis of GTP hydrolysis associated with tubulin polymerization
- User:Andy Maloney/Notebook/Lab Notebook of Andy Maloney/2009/05/08/Ionic and nucleotide requirements for microtubule polymerization in vitro review
- Categories: Kinesin and microtubule papers, Kinesin and microtubules
- Description: Paper review. Ionic and nucleotide requirements for microtubule polymerization in vitro
- User:Andy Maloney/Notebook/Lab Notebook of Andy Maloney/2009/05/11/Mechanism of Microtubule Stabilization by Doublecortin review
- Categories: Kinesin and microtubule papers, Kinesin and microtubules
- Description: Paper review. Mechanism of Microtubule Stabilization by Doublecortin
- User:Andy Maloney/Notebook/Lab Notebook of Andy Maloney/2009/05/22/Laser diode
- Categories: AM Engineering, Diode laser, Koch meeting, Spatial filtering
- Description: Talking about the laser diode and making sure one writes in your notebook even if it's a failure.
- User:Andy Maloney/Notebook/Lab Notebook of Andy Maloney/2009/05/28
- Categories: AM Engineering
- Description: Closed signs vent.
- User:Andy Maloney/Notebook/Lab Notebook of Andy Maloney/2009/06/01/A Polarized Microtubule Array for Kinesin Powered Nanoscale Assembly and Force Generation
- Categories: Kinesin and microtubule papers, Kinesin and microtubules
- Description: Paper review. A Polarized Microtubule Array for Kinesin-Powered Nanoscale Assembly and Force Generation
- User:Andy Maloney/Notebook/Lab Notebook of Andy Maloney/2009/06/01/In the lab
- Categories: AM Engineering
- Description: More lab work stuff.
- User:Andy Maloney/Notebook/Lab Notebook of Andy Maloney/2009/06/02/Measurement of the Force-Velocity Relation for Growing Microtubules
- Categories: Kinesin and microtubule papers, Kinesin and microtubules
- Description: Paper review. Measurement of the Force Velocity Relation for Growing Microtubules
- User:Andy Maloney/Notebook/Lab Notebook of Andy Maloney/2009/06/02/Molecular Shuttles Operating Undercover: A New Photolithographic Approach for the Fabrication of Structured Surfaces Supporting Directed Motility
- User:Andy Maloney/Notebook/Lab Notebook of Andy Maloney/2009/06/02/In the lab
- Categories: AM Engineering
- Description: Ordering stuff for the kinesin project.
- User:Andy Maloney/Notebook/Lab Notebook of Andy Maloney/2009/06/03/Laser diode stuff
- Categories: Beam shaping, Diode laser, Optical tweezers, Sketchup, Spatial filtering
- Description: Attempting to collimate the diode laser with a fiber optic.
- User:Andy Maloney/Notebook/Lab Notebook of Andy Maloney/2009/06/04/Movement of microtubules by single kinesin molecules
- Categories: Kinesin and microtubule papers, Kinesin and microtubules
- Description: Paper review. Movement of microtubules by single kinesin molecules
- User:Andy Maloney/Notebook/Lab Notebook of Andy Maloney/2009/06/04/Taxol stabilization of mitotic spindle microtubules - analysis using calcium induced depolymerization
- Categories: Kinesin and microtubule papers, Kinesin and microtubules
- Description: Paper review. Taxol stabilization of mitotic spindle microtubules: analysis using calcium-induced depolymerization
- User:Andy Maloney/Notebook/Lab Notebook of Andy Maloney/2009/06/04/In the lab
- Categories: AM Engineering, Beam shaping, Diode laser, Optical tweezers
- Description: More attempts at coupling the diode laser to the fiber optic.
- User:Andy Maloney/Notebook/Lab Notebook of Andy Maloney/2009/06/08/Laser diode
- Categories: AM Engineering, Beam shaping, Diode laser, Optical tweezers, Spatial filtering
- Description: I finally was able to get the fiber collimated. But, it's multimode and I won't figure that multimode lasers don't work for optical tweezers.
- User:Andy Maloney/Notebook/Lab Notebook of Andy Maloney/2009/06/09/Please sir, may I have some more laser?
- Categories: AM Engineering, Diode laser, Optical tweezers, Pages with broken file links
- Description: I was able to make an optical cannon with the diode laser.
- User:Andy Maloney/Notebook/Lab Notebook of Andy Maloney/2009/06/11
- Categories: AM Engineering, Diode laser, Optical tweezers
- Description: I was able to tweeze with the diode laser. But, the forces were way to weak to be useful.
- User:Andy Maloney/Notebook/Lab Notebook of Andy Maloney/2009/06/25/Liposomes and kinesin
- Categories: AM Engineering, Kinesin and microtubules, Surface passivation
- Description: Doing calculations on the amount of lipids needed to coat a cover slip. The idea was to use lipids as the passivation for the kinesin and microtubule experiments.
- User:Andy Maloney/Notebook/Lab Notebook of Andy Maloney/2009/06/26/Optical tweezers
- Categories: 1064 optical tweezers, AM Engineering, Optical tweezers
- Description: Since the diode laser proved to not be useful, we got a 1064nm laser to build an optical tweezers.
- User:Andy Maloney/Notebook/Lab Notebook of Andy Maloney/2009/06/27/1064 Optical tweezers
- Categories: 1064 optical tweezers, AM Engineering, Optical tweezers
- Description: Working with the 1064 optical tweezers and the unusual power modulator we borrowed.
- User:Andy Maloney/Notebook/Lab Notebook of Andy Maloney/2009/07/01/1064 optical tweezers
- Categories: AM Engineering, Optical tweezers
- Description: Talking about the periscope, how to align the beam through the microscope and some steering optics.
- User:Andy Maloney/Notebook/Lab Notebook of Andy Maloney/2009/07/03/1064 laser tweezers
- Categories: AM Engineering, Optical tweezers
- Description: Talking more about alignment and that I was able to tweeze with the 1064 laser.
- User:Andy Maloney/Notebook/Lab Notebook of Andy Maloney/2009/07/05/Things to do
- Categories: AM Engineering
- Description: Random stuff to be done listed in this entry.
- User:Andy Maloney/Notebook/Lab Notebook of Andy Maloney/2009/07/06/1064 Optical Tweezers
- Categories: 1064 optical tweezers, AM Engineering, Optical tweezers
- Description: Talking about the condenser adapter piece, detector stage and steering optics.
- User:Andy Maloney/Notebook/Lab Notebook of Andy Maloney/2009/07/07/1064 laser tweezers
- Categories: 1064 optical tweezers, AM Engineering, Optical tweezers
- Description: Got new mirrors in for the periscope.
- User:Andy Maloney/Notebook/Lab Notebook of Andy Maloney/2009/07/08/1064 Optical tweezers
- Categories: 1064 optical tweezers, AM Engineering, Optical tweezers
- Description: Setting up detection optics for the tweezers. Some thoughts on purchases.
- User:Andy Maloney/Notebook/Lab Notebook of Andy Maloney/2009/07/31/Metal chelate chromatography
- Categories: Kinesin and microtubule basics, Kinesin and microtubules
- Description: Learning a little bit about protein purification.
- User:Andy Maloney/Notebook/Lab Notebook of Andy Maloney/2009/07/31/Noisy tweezers
- Categories: AM Engineering, Optical tweezers
- Description: Thoughts on how to get rid of the noise in the tweezers.
- User:Andy Maloney/Notebook/Lab Notebook of Andy Maloney/2009/08/04/Beginning attempts at surface passivation
- Categories: Kinesin and microtubules, Surface passivation
- Description: Beginning attempts at passivating surfaces with lipids. Also, my ideas with spin coatings.
- User:Andy Maloney/Notebook/Lab Notebook of Andy Maloney/2009/08/05/Microscope setup
- Categories: AM Engineering, Kinesin and microtubules
- Description: Issues with visualizing microtubules due to improper installment of the Hg lamp.
- User:Andy Maloney/Notebook/Lab Notebook of Andy Maloney/2009/08/05/Motility experiment
- Categories: Kinesin and microtubules
- Description: My first success at a gliding motility assay.
- User:Andy Maloney/Notebook/Lab Notebook of Andy Maloney/2009/08/06/Kiney computer setup
- Categories: AM Engineering, Kinesin and microtubules
- Description: Talking about the computer we got from surplus for the kinesin experiments.
- User:Andy Maloney/Notebook/Lab Notebook of Andy Maloney/2009/08/07/Spin Coater 3000
- Categories: AM Engineering, Kinesin and microtubules
- Description: Making of the Spin Coater 3000 from my Spin Master Micro.
- User:Andy Maloney/Notebook/Lab Notebook of Andy Maloney/2009/08/10/Gliding motility assay
- Categories: Kinesin and microtubules
- Description: The first movie of a gliding motility assay from KochLab.
- User:Andy Maloney/Notebook/Lab Notebook of Andy Maloney/2009/08/11/Spin coater 3000 Trial 1
- Categories: Kinesin and microtubules, Surface passivation
- Description: Using the Spin Coater 3000.
- User:Andy Maloney/Notebook/Lab Notebook of Andy Maloney/2009/08/12/Surface passivation trial 2
- Categories: Kinesin and microtubules, Surface passivation
- Description: Using the Spin Coater 3000 again. While I was able to coat the slips with lipids, I was never able to get a motility assay going.
- User:Andy Maloney/Notebook/Lab Notebook of Andy Maloney/2009/08/17/Surface passivation
- Categories: Kinesin and microtubules, Surface passivation
- Description: My Spin Coater 3000 didn't cut the mustard. So, we tried using the Fisher centrifuge to coat slips. We were able to do it but, not in a very effective manner.
- User:Andy Maloney/Notebook/Lab Notebook of Andy Maloney/2009/08/19/Surface passivation experiment
- Categories: AM Engineering, Kinesin and microtubules, Surface passivation
- Description: Talking about the whole casein issue and how it doesn't go into solution very easily. I did heat up the solution for the first time and started to question why no one has done this before.
- User:Andy Maloney/Notebook/Lab Notebook of Andy Maloney/2009/08/20/Continued setup for surface passivation
- Categories: Kinesin and microtubules, Surface passivation
- Description: Attempting different concentrations of rhodamine labeled tubulin for fluorescent GMA. Also, I found a webpage and paper that talks about casein in poop. This stemmed from Koch's comment about my heating of PEM to get whole casein to go into solution.
- User:Andy Maloney/Notebook/Lab Notebook of Andy Maloney/2009/08/27/Kinesin walking
- Categories: Kinesin and microtubules
- Description: Attempt to work with Larry on simulating kinesin walking.
- User:Andy Maloney/Notebook/Lab Notebook of Andy Maloney/2009/08/27/Tubulin resuspension
- Categories: Kinesin and microtubules, Surface passivation
- Description: Using kappa casein as the surface passivator. This is before I realized that it doesn't work that well.
- User:Andy Maloney/Notebook/Lab Notebook of Andy Maloney/2009/08/28/Motility assay
- Categories: Kinesin and microtubules, Surface passivation
- Description: My definition for a new naming convention for the buffers used in our experiments. Also, direct confirmation of Hancock's paper about the length of microtubules supported with different bovine caseins.
- User:Andy Maloney/Notebook/Lab Notebook of Andy Maloney/2009/08/31/Passivation studies
- Categories: Kinesin and microtubules, Surface passivation
- Description: Noting my update to the protocol section of KochLab. Also, I lament about my paleness and how overexposure to the sun affects my mental faculties.
- User:Andy Maloney/Notebook/Lab Notebook of Andy Maloney/2009/08/31/Ethics course Lecture 1
- Categories: Ethics course
- Description: Ethics course with a very famous ethics professor.
- User:Andy Maloney/Notebook/Lab Notebook of Andy Maloney/2009/09/01/Kinesin Motor Mechanics: Binding, Stepping, Tracking, Gating, and Limping Review
- Categories: Kinesin and microtubule papers, Kinesin and microtubules, Kinetic Monte Carlo for Kinesin Processivity
- Description: Paper review. Kinesin Motor Mechanics: Binding, Stepping, Tracking, Gating, and Limping
- User:Andy Maloney/Notebook/Lab Notebook of Andy Maloney/2009/09/02/Surface passivation studies
- Categories: Kinesin and microtubules, Surface passivation
- Description: Question the arose from passivation with kappa casein and with lipids. The lipids don't work and the kappa casein does (sort of as I'll find out later).
- User:Andy Maloney/Notebook/Lab Notebook of Andy Maloney/2009/09/02/Ethics course Lecture 2
- Categories: Ethics course
- Description: Ethics course again. Mostly vocabulary that the prof says.
- User:Andy Maloney/Notebook/Lab Notebook of Andy Maloney/2009/09/02/Kinesin walking
- Categories: Kinesin and microtubules, Kinetic Monte Carlo for Kinesin Processivity
- Description: More on the simulation of kinesin walking.
- User:Andy Maloney/Notebook/Lab Notebook of Andy Maloney/2009/09/03
- Categories: Surface passivation
- Description: Notes on what the differences are between a casein passivated surface and a lipid passivated one and why the lipid surface doesn't work.
- User:Andy Maloney/Notebook/Lab Notebook of Andy Maloney/2009/09/08/Surface passivation studies
- Categories: Kinesin and microtubules, Surface passivation
- Description: Thoughts on surface passivation and the differences between hydrophobic/hydrophilic surfaces. I also used potato starch as a surface passivator for some reason.
- User:Andy Maloney/Notebook/Lab Notebook of Andy Maloney/2009/09/09/Surface passivation studies
- Categories: Kinesin and microtubules, Surface passivation
- Description: More thoughts on if you need to passivate both surfaces of a flow cell. Also, I observed what I called "clumping" of microtubules. In fact, these were Taxol crystals masquerading as microtubule clumps. I won't figure that out till later.
- User:Andy Maloney/Notebook/Lab Notebook of Andy Maloney/2009/09/10/Koch mini meeting
- Categories: Kinesin and microtubules, Kochlab meetings
- Description: Meeting with Koch. Discussed slide cleaning, aliquotting, and making our own buffers.
- User:Andy Maloney/Notebook/Lab Notebook of Andy Maloney/2009/09/10/Surface passivation studies
- Categories: Kinesin and microtubules, Surface passivation
- Description: Using different cleaning methods to produce flow cells resulted in no motility. I believed the reason there was no motility was due to bad kinesin.
- User:Andy Maloney/Notebook/Lab Notebook of Andy Maloney/2009/09/11/Surface passivation studies
- Categories: Kinesin and microtubules, Surface passivation
- Description: Slide cleaning and its affects on motility. All experiments stopped working. In hindsight, it was due to a contamination issue.
- User:Andy Maloney/Notebook/Lab Notebook of Andy Maloney/2009/09/14/Getting motility to work again
- Categories: Kinesin and microtubules, Surface passivation
- Description: Still trying to get motility to work again. I bought all new stuff and made all new solutions. This is a reoccurring theme with these assays.
- User:Andy Maloney/Notebook/Lab Notebook of Andy Maloney/2009/09/14/Ethics course 3
- Categories: AM Engineering, Ethics course
- Description: More vocabulary from the ethics professor.
- User:Andy Maloney/Notebook/Lab Notebook of Andy Maloney/2009/09/21/The chemistry of kinesin and microtubule buffers
- Categories: AM Engineering, Kinesin and microtubule basics, Kinesin and microtubules
- Description: Precursor page to my kinesin and microtubule procedure page. Talks about cleaning, the chemicals used in the assay and the buffers used.
- User:Andy Maloney/Notebook/Lab Notebook of Andy Maloney/2009/09/21/Ethics course Lecture 3 or 4
- Categories: AM Engineering, Ethics course
- Description: More vocabulary I mean, more ethics course.
- User:Andy Maloney/Notebook/Lab Notebook of Andy Maloney/2009/09/23/Thoughts on the simulation
- Categories: Kinesin and microtubules, Kinetic Monte Carlo for Kinesin Processivity
- Description: More kinesin simulation ideas.
- User:Andy Maloney/Notebook/Lab Notebook of Andy Maloney/2009/09/23/Websites to emulate
- Categories: AM Engineering
- Description: Ideas about changing the horrid physics website at UNM.
- User:Andy Maloney/Notebook/Lab Notebook of Andy Maloney/2009/09/28
- Categories: AM Engineering
- Description: Lessons on how to be pretentiousness, I mean my ethics course.
- User:Andy Maloney/Notebook/Lab Notebook of Andy Maloney/2009/09/29/New stuff
- Categories: AM Engineering, Andy's stock solutions, Kinesin and microtubules
- Description: Received new chemicals to start making new solutions.
- User:Andy Maloney/Notebook/Lab Notebook of Andy Maloney/2009/09/30/Preparing for experiments
- Categories: AM Engineering, Andy's stock solutions, Kinesin and microtubules
- Description: Making new ATP aliquots. I didn't label my tubes in the pic but I eventually did since you should label everything in the lab.
- User:Andy Maloney/Notebook/Lab Notebook of Andy Maloney/2009/10/01/Motility
- Categories: Kinesin and microtubules
- Description: First success at the GMA after figuring out that our pipettors were contaminated.
- User:Andy Maloney/Notebook/Lab Notebook of Andy Maloney/2009/10/03/The osmotic pressure of concentrated solutions and the laws of the perfect solution
- Categories: Kinesin and microtubules
- Description: Paper review. The osmotic pressure of concentrated solutions, and the laws of the perfect solution
- User:Andy Maloney/Notebook/Lab Notebook of Andy Maloney/2009/10/03/Surface passivation
- Categories: Kinesin and microtubules, Surface passivation
- Description: More surface passivation stuff. This time with silica nanoparticles.
- User:Andy Maloney/Notebook/Lab Notebook of Andy Maloney/2009/10/04/2010 Biophysical Society Abstract
- Categories: Kinesin and microtubules
- Description: Koch's 2010 Biophysics abstract corrections for my poster. Also, this page uses Google Docs.
- User:Andy Maloney/Notebook/Lab Notebook of Andy Maloney/2009/10/13/Lipid preps
- Categories: Kinesin and microtubules, Surface passivation
- Description: Surface passivation with lipids still not working.
- User:Andy Maloney/Notebook/Lab Notebook of Andy Maloney/2009/10/14/Lipid surface passivation
- Categories: Kinesin and microtubules, Surface passivation
- Description: Studies with lipids. I will point out that the lipid surfaces do make microtubules stick similar to poly-L-lysine. But, the antifade is just rotten when using a lipid surface.
- User:Andy Maloney/Notebook/Lab Notebook of Andy Maloney/2009/10/15/Lipid passivation
- Categories: Kinesin and microtubules, Surface passivation
- Description: More lipid stuff.
- User:Andy Maloney/Notebook/Lab Notebook of Andy Maloney/2009/10/16/Lipid passivation
- Categories: Kinesin and microtubules, Surface passivation
- Description: My first realization that Taxol solutions should not sit around for very long.
- User:Andy Maloney/Notebook/Lab Notebook of Andy Maloney/2009/10/19/Lipids discussion
- Categories: Kinesin and microtubules, Surface passivation
- Description: Some jibba jabba on lipids.
- User:Andy Maloney/Notebook/Lab Notebook of Andy Maloney/2009/10/19/Operation graduation
- Categories: AM Engineering
- Description: Plans for gradu...hahahahahaha...tion.
- User:Andy Maloney/Notebook/Lab Notebook of Andy Maloney/2009/10/20/Heavy water
- Categories: AM D2O, AM Engineering, Kinesin and microtubules
- Description: Preparation of Heavy-PEM.
- User:Andy Maloney/Notebook/Lab Notebook of Andy Maloney/2009/10/21/Heavy PEM
- Categories: AM D2O, Kinesin and microtubules
- Description: First attempts with Heavy-PEM. But, I'm having problems with the kappa casein passivation.
- User:Andy Maloney/Notebook/Lab Notebook of Andy Maloney/2009/10/23/Protocols
- Categories: AM Engineering
- Description: Talking about a new protocol page for my flow cells.
- User:Andy Maloney/Notebook/Lab Notebook of Andy Maloney/2009/10/23/Autoclave
- Categories: AM Engineering
- Description: Talking about how dirty our autoclave is and how it may affect my stuff.
- User:Andy Maloney/Notebook/Lab Notebook of Andy Maloney/2009/10/23/Motility
- Categories: Kinesin and microtubules
- Description: Getting motility working. Of course I now know that it is because of the kappa casein passivation.
- User:Andy Maloney/Notebook/Lab Notebook of Andy Maloney/2009/10/24
- Categories: AM Engineering
- Description: Irritating pest problem.
- User:Andy Maloney/Notebook/Lab Notebook of Andy Maloney/2009/10/28
- Categories: AM Engineering
- Description: Listing things to do.
- User:Andy Maloney/Notebook/Lab Notebook of Andy Maloney/2009/10/28/Lipid comparisons
- Categories: Kinesin and microtubules, Surface passivation
- Description: Success with motility experiments. Not so much success with lipid trials.
- User:Andy Maloney/Notebook/Lab Notebook of Andy Maloney/2009/10/29
- Categories: AM Engineering
- Description: To do list.
- User:Andy Maloney/Notebook/Lab Notebook of Andy Maloney/2009/10/29/Lipid stuff
- Categories: AM Engineering, Kinesin and microtubules, Surface passivation
- Description: Lamenting on lipid solubility and my procedures for getting them into solution. Also, more computer issues with the microscope setup.
- User:Andy Maloney/Notebook/Lab Notebook of Andy Maloney/2009/10/30/Heavy motility
- Categories: AM D2O, Kinesin and microtubules
- Description: First real experiments using D2O in my PEM solution.
- User:Andy Maloney/Notebook/Lab Notebook of Andy Maloney/2009/10/31/This is insane
- Categories: AM D2O, Kinesin and microtubules
- Description: I got motility in a solution of D2O one day later. Tests with and without AF in Heavy-PEM.
- User:Andy Maloney/Notebook/Lab Notebook of Andy Maloney/2009/11/02/More osmotic pressure studies
- Categories: AM D2O, Kinesin and microtubules
- Description: Showing microtubules last in D2O for a long period of time. Showed that MTs will polymerize in D2O.
- User:Andy Maloney/Notebook/Lab Notebook of Andy Maloney/2009/11/03/Microtubule studies
- Categories: AM D2O, Kinesin and microtubules
- Description: More thoughts on measuring things in Heavy-PEM.
- User:Andy Maloney/Notebook/Lab Notebook of Andy Maloney/2009/11/14
- Categories: AM Engineering, Kinesin and microtubules
- Description: Posters for the DTRA meeting.
- User:Andy Maloney/Notebook/Lab Notebook of Andy Maloney/2009/11/16/Dallas movies
- Categories: Kinesin and microtubules
- Description: Movies to show people at the DTRA meeting.
- User:Andy Maloney/Notebook/Lab Notebook of Andy Maloney/2009/11/25/Sucrose
- Categories: AM OsmoticStress, Kinesin and microtubules
- Description: Using sucrose as an osmolyte in the gliding motility assays.
- User:Andy Maloney/Notebook/Lab Notebook of Andy Maloney/2009/12/01/Taxol Crystals Can Masquerade as Stabilized Microtubules
- Categories: Kinesin and microtubule papers, Kinesin and microtubules
- Description: Paper review. Taxol Crystals Can Masquerade as Stabilized Microtubules
- User:Andy Maloney/Notebook/Lab Notebook of Andy Maloney/2009/12/01/Tubulin Dimers Oligomerize before Their Incorporation into Microtubules
- Categories: Kinesin and microtubule papers, Kinesin and microtubules
- Description: Paper review. Tubulin Dimers Oligomerize before Their Incorporation into Microtubules
- User:Andy Maloney/Notebook/Lab Notebook of Andy Maloney/2009/12/07/Effect of Sodium Chloride on the Solubility of Caseins
- Categories: Kinesin and microtubule papers, Kinesin and microtubules
- Description: Paper review. Effect of Sodium Chloride on the Solubility of Caseins
- User:Andy Maloney/Notebook/Lab Notebook of Andy Maloney/2009/12/08/50 Point
- Categories: Heavy water K&MTs, Kinesin and microtubules, Surface passivation
- Description: Comparison of whole casein and kappa casein passivation.
- User:Andy Maloney/Notebook/Lab Notebook of Andy Maloney/2009/12/09/50 D2O
- Categories: AM D2O, Heavy water K&MTs, Kinesin and microtubules
- Description: Investigating the unusual drop in the 50% D2O data.
- User:Andy Maloney/Notebook/Lab Notebook of Andy Maloney/2009/12/11
- Categories: AM Engineering
- Description: Looking at how permalinks work.
- User:Andy Maloney/Notebook/Lab Notebook of Andy Maloney/2009/12/29
- Categories: AM Engineering, Kinesin and microtubules
- Description: Looking at how median filtering works. We ultimately don't use this technique anymore for our data analysis.
- User:Andy Maloney/Notebook/Lab Notebook of Andy Maloney/2010/01/07/Surface passivation idea
- Categories: Kinesin and microtubules, Surface passivation
- Description: Really starting to look at caseins. Abandoning lipids and looking at sialic acids.
- User:Andy Maloney/Notebook/Lab Notebook of Andy Maloney/2010/01/12/OWW suggestions
- Categories: AM Engineering
- Description: Silly idea that I could possibly be able to suggest improvements to OWW.
- User:Andy Maloney/Kinesin & Microtubule Page/Osmotic stress/Analysis of the gliding fishtailing and circling motions of native microtubules
- Categories: Kinesin and microtubule papers, Kinesin and microtubules
- Description: Paper review. Analysis of the gliding, fishtailing and circling motions of native microtubules.
- User:Andy Maloney/Notebook/Lab Notebook of Andy Maloney/2010/01/19/Phase grating
- Categories: AM Engineering, Optical tweezers
- Description: A description of what a phase grating is.
- User:Andy Maloney/Notebook/Lab Notebook of Andy Maloney/2010/01/20/Casein constituents
- Categories: AM Engineering, Kinesin and microtubules, Surface passivation
- Description: Received casein constituents in today and made 0.5mg/mL PEM solutions with them in it.
- User:Andy Maloney/Notebook/Lab Notebook of Andy Maloney/2010/01/26/Caseins
- Categories: Kinesin and microtubules, Surface passivation
- Description: First experiment to compare the different constituents of bovine casein as a surface passivator.
- User:Andy Maloney/Notebook/Lab Notebook of Andy Maloney/2010/01/28
- Categories: Kinesin and microtubules
- Description: Jotting down ideas and references.
- User:Andy Maloney/Notebook/Lab Notebook of Andy Maloney/2010/01/29
- Categories: AM Engineering
- Description: Testing embedding Friendfeed threads in my notebook.
- User:Andy Maloney/Kinesin & Microtubule Page/Microtubule papers/Kinesin follows the microtubules protofilament axis
- Categories: Kinesin and microtubule basics, Kinesin and microtubule papers, Kinesin and microtubules
- Description: Paper review. Kinesin follows the microtubule's protofilament axis
- User:Andy Maloney/Notebook/Lab Notebook of Andy Maloney/2010/02/04
- Categories: Kinesin and microtubules
- Description: First data with using whole casein. The data shows a huge spread in speed.
- User:Andy Maloney/Notebook/Lab Notebook of Andy Maloney/2010/02/05
- Categories: Kinesin and microtubules
- Description: Looking at a motility assay for an extended period of time.
- User:Andy Maloney/Notebook/Lab Notebook of Andy Maloney/2010/02/10
- Categories: AM Engineering
- Description: Playing around with Google Buzz embedding.
- User:Andy Maloney/Notebook/Lab Notebook of Andy Maloney/2010/02/10/PBL1
- Categories: AM Engineering, Nanoparticles course
- Description: Irritating classwork.
- User:Andy Maloney/Notebook/Lab Notebook of Andy Maloney/2010/02/12
- Categories: Kinesin and microtubules
- Description: Seeing more odd speed increases over the time course of an assay. Still don't know what it is. Using whole casein as the surface passivator.
- User:Andy Maloney/Notebook/Lab Notebook of Andy Maloney/2010/03/01/Problem based learning 2
- Categories: AM Engineering
- Description: More irritating classwork.
- User:Andy Maloney/Notebook/Lab Notebook of Andy Maloney/2010/03/04/MT speed and passivation
- Categories: Kinesin and microtubules, Surface passivation
- Description: Looking at all the different casein solutions. I believe that at this time I was still putting casein in my motility solution. I do not do this anymore.
- User:Andy Maloney/Notebook/Lab Notebook of Andy Maloney/2010/03/08/Fixed microtubules
- Categories: AM Engineering, Kinesin and microtubules
- Description: Trying to use poly-L-lysine to fix microtubules to the glass slides. Also looking at speckling on microtubules due to where the rhodamine labels are when I polymerize them.
- User:Andy Maloney/Notebook/Lab Notebook of Andy Maloney/2010/03/17/ReAutomation
- Categories: Kinesin and microtubules, Surface passivation
- Description: First look at gliding motility speeds using the different bovine caseins as passivators. Still no temperature stabilization for this data.
- User:Andy Maloney/Notebook/Lab Notebook of Andy Maloney/2010/03/17/Poly-L-lysine
- Categories: Kinesin and microtubules
- Description: Using poly-L-lysine on the glass surface to fix microtubules.
- User:Andy Maloney/Notebook/Lab Notebook of Andy Maloney/2010/03/26/Dr Dill meeting
- Categories: AM OsmoticStress, Kinesin and microtubules
- Description: Notes from our meeting with Ken Dill.
- User:Andy Maloney/Notebook/Lab Notebook of Andy Maloney/2010/04/01/Casein assay 2
- Categories: Kinesin and microtubules, Surface passivation
- Description: Redoing the casein assay from 2010-03-04.
- User:Andy Maloney/Notebook/Lab Notebook of Andy Maloney/2010/04/01/Osmotic stress planning
- Categories: AM OsmoticStress, Kinesin and microtubules
- Description: This notebook entry plans for using betaine as an osmotic stessor and I note preliminary results.
- User:Andy Maloney/Notebook/Lab Notebook of Andy Maloney/2010/04/02/Osmotic stress mapping
- Categories: AM OsmoticStress, Kinesin and microtubules
- Description: This notebook entry is basically repeating April 1st entry in regards to writing again what I want to do with the osmotic stress experiment.
- User:Andy Maloney/Notebook/Lab Notebook of Andy Maloney/2010/04/02/Heavy water mapping
- Categories: AM D2O, AM HeavyWater, Kinesin and microtubules
- Description: Jotting down ideas for the heavy water experiment.
- User:Andy Maloney/Notebook/Lab Notebook of Andy Maloney/2010/04/02/Updated casein data
- Categories: Kinesin and microtubules, Surface passivation
- Description: This page shows some updated casein data.
- User:Andy Maloney/Notebook/Lab Notebook of Andy Maloney/2010/04/13
- Categories: Kinesin and microtubules
- Description: Looking at data using histograms.
- User:Andy Maloney/Notebook/Lab Notebook of Andy Maloney/2010/05/24/Objective temperature control
- Categories: AM Surface passivation paper, AM Temp effects on kinesin velocity, Kinesin and microtubules
- Description: Mapping out what I should do about stabilizing the temperature of the objective. Also, this page describes my initial study on temperature affects.
- User:Andy Maloney/Notebook/Lab Notebook of Andy Maloney/2010/05/25/More on kinesin speed effects due to temperature
- Categories: AM Surface passivation paper, AM Temp effects on kinesin velocity, Kinesin and microtubules
- Description: his is a follow up to the initial study of temperature issues.
- User:Andy Maloney/Notebook/Lab Notebook of Andy Maloney/2010/05/25/Speeding up kinesin-driven microtubule gliding in vitro by variation of cofactor composition and physicochemical parameters
- User:Andy Maloney/Notebook/Lab Notebook of Andy Maloney/2010/05/25/LED illumination for video-enhanced DIC imaging of single microtubules
- Categories: AM Temp effects on kinesin velocity, Kinesin and microtubule papers, Kinesin and microtubules
- Description: Paper review. LED illumination for video-enhanced DIC imaging of single microtubules
- User:Andy Maloney/Notebook/Lab Notebook of Andy Maloney/2010/05/27/Casein data revisited
- Categories: AM Surface passivation paper, AM Temp effects on kinesin velocity, Kinesin and microtubules, Surface passivation
- Description: Reanalyzing the casein data I took to incorporate the times I took the data at. This shows a temperature affect going on.
- User:Andy Maloney/Notebook/Lab Notebook of Andy Maloney/2010/06/07/LED for microscope temperature control
- Categories: AM Surface passivation paper, AM Temp effects on kinesin velocity, Kinesin and microtubules
- Description: This is my first look at how the Thorlabs LED controller works. The goal is to replace the existing LED with another LED so that I can get a broad spectrum of colors in the microscope.
- User:Andy Maloney/Notebook/Lab Notebook of Andy Maloney/2010/06/17/Temperature stabilization
- Categories: AM Surface passivation paper, AM Temp effects on kinesin velocity, Kinesin and microtubules
- Description: Objective temperature stabilization build, slide warmer, and LED discussions.
- User:Andy Maloney/Notebook/Lab Notebook of Andy Maloney/2010/06/23/Temperature data
- Categories: AM Surface passivation paper, AM Temp effects on kinesin velocity, Kinesin and microtubules
- Description: Posting data with temperature stabilization as well as looking more at the LED from Bridgelux. Also, an experiment where I look at only one spot in the sample.
- User:Andy Maloney/Notebook/Lab Notebook of Andy Maloney/2010/06/25/LED and Spot test
- Categories: AM Surface passivation paper, Kinesin and microtubules
- Description: Data from the other day where I looked at GMA with an LED and continued illumination with the Hg lamp.
- User:Andy Maloney/Notebook/Lab Notebook of Andy Maloney/2010/06/29/Whole casein with temperature stabilization
- Categories: AM Surface passivation paper, AM Temp effects on kinesin velocity, Kinesin and microtubules
- Description: I now have repeated experiments where I have stabilized the temperature of the objective and use whole casein as the passivation.
- User:Andy Maloney/Notebook/Lab Notebook of Andy Maloney/2010/07/19
- Categories: AM Surface passivation paper, AM Temp effects on kinesin velocity, Kinesin and microtubules
- Description: Beginning to notice that my casein solutions are no longer working. All I changed was the temperature stabilization.
- User:Andy Maloney/Notebook/Lab Notebook of Andy Maloney/2010/07/20
- Categories: AM Engineering, AM Surface passivation paper, Kinesin and microtubules
- Description: Making new solutions.
- User:Andy Maloney/Notebook/Lab Notebook of Andy Maloney/2010/07/26/Notebook Entries
- Categories: AM Engineering, Kinesin and microtubules
- Description: Friendfeed thread talking about notebook entries
- User:Andy Maloney/Notebook/Lab Notebook of Andy Maloney/2010/07/26/Whole casein failure
- Categories: AM Engineering, AM SurfacePassivationMTs&Kinesin, AM Surface passivation paper, Kinesin and microtubule basics, Kinesin and microtubules
- Description: Making W-PEM and the failures that I encountered making it.
- User:Andy Maloney/Notebook/Lab Notebook of Andy Maloney/2010/07/26/Microscope repair
- Categories: AM Engineering, AM Surface passivation paper, Kinesin and microtubules, Optical tweezers
- Description: Repairing an IX71 microscope.
- User:Andy Maloney/Notebook/Lab Notebook of Andy Maloney/2010/07/27/Reboot
- Categories: AM Surface passivation paper, Kinesin and microtubules, Surface passivation
- Description: Initial test to see if all my new solutions work. No data.
- User:Andy Maloney/Notebook/Lab Notebook of Andy Maloney/2010/07/28/Updated reboot
- Categories: Kinesin and microtubules, Surface passivation
- Description: Data from yesterday's reboot. Looking at all new solutions. Whole casein speed data.
- User:Andy Maloney/Notebook/Lab Notebook of Andy Maloney/2010/07/28/Caseins
- Categories: AM Surface passivation paper, Kinesin and microtubules, Surface passivation
- Description: Looking at casein passivation with all the new solutions made. Beta, alpha, whole so far. Not looking so good.
- User:Andy Maloney/Notebook/Lab Notebook of Andy Maloney/2010/07/29/Beta casein test
- Categories: AM Surface passivation paper, Kinesin and microtubules, Surface passivation
- Description: Trying the beta casein assay without putting it in the freezer and without filtering it and not putting it in the freezer. Guess what, it was the filtering.
- User:Andy Maloney/Notebook/Lab Notebook of Andy Maloney/2010/07/30
- Categories: AM Engineering
- Description: Noting Google's ability for me to upload my data in movie form to my account.
- User:Andy Maloney/Notebook/Lab Notebook of Andy Maloney/2010/08/03/Temperature probe
- Categories: AM Engineering, AM Surface passivation paper, Kinesin and microtubules
- Description: Getting the temperature probe working for the temperature stabilized objective.
- User:Andy Maloney/Notebook/Lab Notebook of Andy Maloney/2010/08/04/Solutions
- Categories: AM Engineering, AM Surface passivation paper, Kinesin and microtubules, Surface passivation
- Description: Again with the remaking of buffers and solutions.
- User:Andy Maloney/Notebook/Lab Notebook of Andy Maloney/2010/08/05/Solutions
- Categories: AM Surface passivation paper, Kinesin and microtubules, Surface passivation
- Description: Finished with remaking solutions.
- User:Andy Maloney/Notebook/Lab Notebook of Andy Maloney/2010/08/05/Casein experiments
- Categories: AM Surface passivation paper, Kinesin and microtubules, Surface passivation
- Description: Noting that the temperature I have the objective at is not hot enough to have a stable speed measurement.
- User:Andy Maloney/Notebook/Lab Notebook of Andy Maloney/2009/05/11/Kinesin and microtubule questions
- Categories: Kinesin and microtubule basics, Kinesin and microtubule papers, Kinesin and microtubules
- Description: Questions about kinesin and microtubules.
- User:Andy Maloney/Notebook/Lab Notebook of Andy Maloney/2010/08/06/Temperature stabilization
- Categories: AM Engineering, AM Surface passivation paper, Kinesin and microtubules
- Description: Epoxied the second thermistor to the objective since the oil used kept seeping under the tape.
- User:Andy Maloney/Notebook/Lab Notebook of Andy Maloney/2010/08/06/Casein studies
- Categories: AM Surface passivation paper, Kinesin and microtubules, Surface passivation
- Description: More casein studies with temperature stabilization. The beta casein assay is still acting "funny", or so I think.
- User:Andy Maloney/Notebook/Lab Notebook of Andy Maloney/2010/08/07/Passivation study
- Categories: AM Surface passivation paper, Kinesin and microtubules, Surface passivation
- Description: More surface passivation studies. I've figured out that the harder it is for a casein constituent to go into solution, the better it acts as a surface passivator. Plus, whole and alpha casein work great. Beta and kappa, not so much. Whole and alpha have motility at the center of the flow cell, beta and kappa has it only near the tape.
- User:Andy Maloney/Notebook/Lab Notebook of Andy Maloney/2010/08/08
- Categories: AM Engineering
- Description: Finally finished tagging my notebook entries.
- User:Andy Maloney/Notebook/Lab Notebook of Andy Maloney/2010/08/10/Casein study
- Categories: AM Surface passivation paper, Kinesin and microtubules, Surface passivation
- Description: Continued experiments using the different caseins as surface passivators.
- User:Andy Maloney/Notebook/Lab Notebook of Andy Maloney/2010/08/11/More caseins
- Categories: AM Engineering, AM Surface passivation paper, Kinesin and microtubules, Surface passivation
- Description: Taking the last bits of data for the bovine casein comparison experiment.
- User:Andy Maloney/Notebook/Lab Notebook of Andy Maloney/2010/08/12/PLoS ONE Requirements
- Categories: AM Engineering
- Description: Shorter description for the requirements for submitting a paper to PLoS ONE.
- User:Andy Maloney/Notebook/Lab Notebook of Andy Maloney/2010/08/12/Casein study
- Categories: AM Surface passivation paper, Kinesin and microtubules, Surface passivation
- Description: Continuing the data taking for my passivation study. I've noticed that kappa and beta casein don't work very well when incubated at room temp. So, I'm going to try the cold thing again.
- User:Andy Maloney/Notebook/Lab Notebook of Andy Maloney/2010/08/13/More casein data taking
- Categories: AM Engineering, AM Surface passivation paper, Kinesin and microtubules, Surface passivation
- Description: Getting the last data points for the passivation study. Also, dealing with the virtualbox update. And, making a stage micrometer.
- User:Andy Maloney/Notebook/Lab Notebook of Andy Maloney/2010/08/26/Group meeting
- Categories: AM Engineering, Kinesin and microtubules
- Description: Group meeting.
- User:Andy Maloney/Notebook/Lab Notebook of Andy Maloney/2010/09/07/Casein data
- Categories: AM Surface passivation paper, Kinesin and microtubules, Surface passivation
- Description: Looking at the movies I've made for the passivation study. I'm going to try an catalog everything I see in them.
- User:Andy Maloney/Notebook/Lab Notebook of Andy Maloney/2010/09/17/Gliding motility assay movie
- Categories: AM Engineering, Kinesin and microtubules, Surface passivation
- Description: Short cartoon of the gliding motiltiy assay.
- User:Andy Maloney/Notebook/Lab Notebook of Andy Maloney/2010/09/21/D2O experiments
- Categories: AM Deuterium effects on kinesin and microtubules, Kinesin and microtubules
- Description: Preparation of stock solutions for heavy hydrogen PEM (H-PEM) and deuterium depleted PEM (DD-PEM). Also, some experiments.
- User:Andy Maloney/Notebook/Lab Notebook of Andy Maloney/2010/09/22/Deuterium effects
- Categories: AM Deuterium effects on kinesin and microtubules, Kinesin and microtubules
- Description: More experiments with deuterium.
- User:Andy Maloney/Notebook/Lab Notebook of Andy Maloney/2010/10/04
- Categories: AM Engineering, Kinesin and microtubules
- Description: Working with Nadia about slide cleaning and the kinesin page. Also, looking at how to automate the tobacco seed experiment.
- User:Andy Maloney/Notebook/Lab Notebook of Andy Maloney/2010/10/05/Preliminary water isotope data
- Categories: AM Water isotope study, Kinesin and microtubules
- Description: Looking at the data I did for initial experiments using water isotopes.
- User:Andy Maloney/Notebook/Lab Notebook of Andy Maloney/2010/10/11/Water isotope experiment
- Categories: AM Water isotope study, Kinesin and microtubules
- Description: Second day doing the water isotope experiments.
- User:Andy Maloney/Notebook/Lab Notebook of Andy Maloney/2010/10/12/Water isotope study
- Categories: AM Engineering, AM Water isotope study, Kinesin and microtubules
- Description: Continuing the water isotope studies.
- User:Andy Maloney/Notebook/Lab Notebook of Andy Maloney/2010/10/19/Water isotope study
- Categories: AM Water isotope study, Kinesin and microtubules
- Description: Continuation of the water isotope study. With a pretty amazing screw up that has prevented polymerization of the microtubules.
- User:Andy Maloney/Notebook/Lab Notebook of Andy Maloney/2010/10/21/New tubulin
- Categories: AM Water isotope study, Kinesin and microtubules
- Description: The new tubulin is in and I suspend it
- User:Andy Maloney/Notebook/Lab Notebook of Andy Maloney/2010/10/23
- Categories: AM Surface passivation paper, Kinesin and microtubules, Surface passivation
- Description: Today I tagged all my notebook entries that pertain to the surface passivation paper.
- User:Andy Maloney/Notebook/Lab Notebook of Andy Maloney/2010/10/25/Tobacco seed chamber
- Categories: AM tobacco seeds
- Description: Talking about the production of the tobacco seed chambers.
- User:Andy Maloney/Notebook/Lab Notebook of Andy Maloney/2010/10/26/Tobacco seed chambers
- Categories: AM tobacco seeds
- Description: More about the tobacco seed chambers.
- User:Andy Maloney/Notebook/Lab Notebook of Andy Maloney/2010/11/02/Water isotope study
- Categories: AM Water isotope study, Kinesin and microtubules
- Description: More water isotope experiments. Plus, experiments where I had to make more O-PEM.
- User:Andy Maloney/Notebook/Lab Notebook of Andy Maloney/2010/11/03/Water isotope study
- Categories: AM Water isotope study, Kinesin and microtubules
- Description: More heavy oxygen water isotope studies.
- User:Andy Maloney/Notebook/Lab Notebook of Andy Maloney/2010/11/05/Water isotope study
- Categories: AM Water isotope study, Kinesin and microtubules
- Description: More water isotope assays. This is the ones with H-PEM.
- User:Andy Maloney/Notebook/Lab Notebook of Andy Maloney/2010/11/09/Water isotope study
- Categories: AM Water isotope study, Kinesin and microtubules
- Description: More data taking.
- User:Andy Maloney/Notebook/Lab Notebook of Andy Maloney/2010/11/10/Water isotope study
- Categories: AM Water isotope study, Kinesin and microtubules
- Description: More data taking.
- User:Andy Maloney/Notebook/Lab Notebook of Andy Maloney/2010/11/11/Water isotope study
- Categories: AM Water isotope study, Kinesin and microtubules
- Description: More data taking for the water isotope study.
- User:Andy Maloney/Notebook/Lab Notebook of Andy Maloney/2011/01/20/Reusable flow cell
- Categories: AM Water isotope study, Kinesin and microtubules
- Description: Looking at how to make a reusable flow cell.
- User:Andy Maloney/Notebook/Lab Notebook of Andy Maloney/2011/01/21/New flow cell
- Categories: AM Engineering, AM Water isotope study, Kinesin and microtubules
- Description: Continued looking into the flow cell that can exchange fluid for more than one experiment.
- User:Andy Maloney/Notebook/Lab Notebook of Andy Maloney/2011/01/24/Reusable flow cell
- Categories: AM Engineering, AM Water isotope study, Kinesin and microtubules
- Description: Mapping what to do with the new reusable flow cell.
- User:Andy Maloney/Notebook/Lab Notebook of Andy Maloney/2011/01/25/Reusable flow cell
- Categories: AM Engineering, AM Water isotope study, Kinesin and microtubules
- Description: Testing the reusable flow cell.
- User:Andy Maloney/Notebook/Lab Notebook of Andy Maloney/2011/01/26/Resealable flow cell
- Categories: AM Engineering, AM Water isotope study, Kinesin and microtubules
- Description: Showing data about the resealable flow cell.
- User:Andy Maloney/Notebook/Lab Notebook of Andy Maloney/2011/01/27/Heavy water storage
- Categories: AM Heavy water storage, Kinesin and microtubules
- Description: Starting the observation of proteins in heavy water storage.
- User:Andy Maloney/Notebook/Lab Notebook of Andy Maloney/2011/01/28/Resealable flow cell
- Categories: AM Engineering, AM Water isotope study, Kinesin and microtubules
- Description: Another round of data taking with the resealable flow cell.
- User:Andy Maloney/Notebook/Lab Notebook of Andy Maloney/2011/02/08/Water isotope experiment
- Categories: AM Water isotope study, Kinesin and microtubules
- Description: Planning what needs to be done for the water isotope experiment.
- User:Andy Maloney/Notebook/Lab Notebook of Andy Maloney/2011/02/10/New stock solutions
- Categories: AM Engineering, AM Water isotope study, Kinesin and microtubules
- Description: Making new stock solutions
- User:Andy Maloney/Notebook/Lab Notebook of Andy Maloney/2011/04/18/Installing Java
- Categories: AM Java
- Description: This page describes my install of Eclipse on Linux and the typical "hello world" first program.
- User:Andy Maloney/Notebook/Lab Notebook of Andy Maloney/2011/04/17
- Categories: AM Java
- Description: test
- User:Andy Maloney/Notebook/Lab Notebook of Andy Maloney/2011/04/20/Tobacco seed movies
- Categories: AM Java, AM Tobacco seeds
- Description: Using Java to make movies of the tobacco seed growth in heavy water.
- User:Andy Maloney/Notebook/Lab Notebook of Andy Maloney/2011/04/21/Learning how to sort in Java
- Categories: AM Java, AM Tobacco seeds
- Description: Continuation from yesterday on learning how to sort in Java.
You can see that if a page does not have any categories listed in it, dpl returns nothing. You can also refine the listing by adding the category= command as given below. Now the only thing that shows up is the page where I have both my template and the category=Kinesin and microtubules.
- User:Andy Maloney/Notebook/Lab Notebook of Andy Maloney/2009/03/12/Kinesin & Microtubules
- Categories: Kinesin and microtubule basics, Kinesin and microtubule papers, Kinesin and microtubules
- Description: One of the first papers I reviewed. Nanomechanics of Microtubules
- User:Andy Maloney/Notebook/Lab Notebook of Andy Maloney/2009/03/17/Kinesin & Microtubules
- Categories: Kinesin and microtubule basics, Kinesin and microtubule papers, Kinesin and microtubules
- Description: Paper review. Kinesin Movement on Glutaraldehyde-Fixed Microtubules
- User:Andy Maloney/Notebook/Lab Notebook of Andy Maloney/2009/04/10/Kinesin & Microtubules
- Categories: Kinesin and microtubule basics, Kinesin and microtubule papers, Kinesin and microtubules
- Description: Paper review. Equilibrium and Transition between Single- and Double-Headed Binding of Kinesin as Revealed by Single-Molecule Mechanics
- User:Andy Maloney/Notebook/Lab Notebook of Andy Maloney/2009/04/13/Kinesin & Microtubules
- Categories: Kinesin and microtubule basics, Kinesin and microtubule papers, Kinesin and microtubules
- Description: Paper review. This is in my notebook twice. Since I'm labeling things in hindsight, I'm not sure why this is the case. Equilibrium and Transition between Single- and Double-Headed Binding of Kinesin as Revealed by Single-Molecule Mechanics
- User:Andy Maloney/Notebook/Lab Notebook of Andy Maloney/2009/04/15/Kinesin and µ-tubules
- Categories: Kinesin and microtubule papers, Kinesin and microtubules
- Description: Paper review. Equilibrium and Transition between Single- and Double-Headed Binding of Kinesin as Revealed by Single-Molecule Mechanics
- User:Andy Maloney/Notebook/Lab Notebook of Andy Maloney/2009/04/16/Kinesin & Microtubules
- Categories: Kinesin and microtubule papers, Kinesin and microtubules
- Description: Paper review. Direct observation of kinesin stepping by optical trapping interferometry
- User:Andy Maloney/Notebook/Lab Notebook of Andy Maloney/2009/04/20/Microtubule paper review
- Categories: AM SurfacePassivationMTs&Kinesin, Kinesin and microtubule basics, Kinesin and microtubule papers, Kinesin and microtubules, Kochlab questions
- Description: Paper review and thoughts about casein.
- User:Andy Maloney/Notebook/Lab Notebook of Andy Maloney/2009/04/21/Kinesin & Microtubules
- Categories: Kinesin and microtubule papers, Kinesin and microtubules, Kochlab questions
- Description: Paper review. How calcium causes microtubule depolymerization
- User:Andy Maloney/Notebook/Lab Notebook of Andy Maloney/2009/04/22/Kinesin & Microtubules
- Categories: Kinesin and microtubule basics, Kinesin and microtubules
- Description: Talking about the buffers used for the gliding motility assay.
- User:Andy Maloney/Notebook/Lab Notebook of Andy Maloney/2009/04/23/The role of casein in supporting the operation of surface bound kinesin
- Categories: Kinesin and microtubule papers, Kinesin and microtubules
- Description: Paper review. The role of casein in supporting the operation of surface bound kinesin
- User:Andy Maloney/Notebook/Lab Notebook of Andy Maloney/2009/04/24/More on kinesin & microtubule buffers
- Categories: Kinesin and microtubule basics, Kinesin and microtubules, Surface passivation
- Description: Found a website talking about caseins.
- User:Andy Maloney/Notebook/Lab Notebook of Andy Maloney/2009/04/28/Koch Lab Kinesin Logo
- Categories: AM Engineering, Kinesin and microtubules
- Description: Birth of Kiney.
- User:Andy Maloney/Notebook/Lab Notebook of Andy Maloney/2009/05/08/Kinetic analysis of GTP hydrolysis associated with tubulin polymerization review
- Categories: Kinesin and microtubule papers, Kinesin and microtubules
- Description: Paper review. Kinetic analysis of GTP hydrolysis associated with tubulin polymerization
- User:Andy Maloney/Notebook/Lab Notebook of Andy Maloney/2009/05/08/Ionic and nucleotide requirements for microtubule polymerization in vitro review
- Categories: Kinesin and microtubule papers, Kinesin and microtubules
- Description: Paper review. Ionic and nucleotide requirements for microtubule polymerization in vitro
- User:Andy Maloney/Notebook/Lab Notebook of Andy Maloney/2009/05/11/Mechanism of Microtubule Stabilization by Doublecortin review
- Categories: Kinesin and microtubule papers, Kinesin and microtubules
- Description: Paper review. Mechanism of Microtubule Stabilization by Doublecortin
- User:Andy Maloney/Notebook/Lab Notebook of Andy Maloney/2009/06/01/A Polarized Microtubule Array for Kinesin Powered Nanoscale Assembly and Force Generation
- Categories: Kinesin and microtubule papers, Kinesin and microtubules
- Description: Paper review. A Polarized Microtubule Array for Kinesin-Powered Nanoscale Assembly and Force Generation
- User:Andy Maloney/Notebook/Lab Notebook of Andy Maloney/2009/06/02/Measurement of the Force-Velocity Relation for Growing Microtubules
- Categories: Kinesin and microtubule papers, Kinesin and microtubules
- Description: Paper review. Measurement of the Force Velocity Relation for Growing Microtubules
- User:Andy Maloney/Notebook/Lab Notebook of Andy Maloney/2009/06/02/Molecular Shuttles Operating Undercover: A New Photolithographic Approach for the Fabrication of Structured Surfaces Supporting Directed Motility
- User:Andy Maloney/Notebook/Lab Notebook of Andy Maloney/2009/06/04/Movement of microtubules by single kinesin molecules
- Categories: Kinesin and microtubule papers, Kinesin and microtubules
- Description: Paper review. Movement of microtubules by single kinesin molecules
- User:Andy Maloney/Notebook/Lab Notebook of Andy Maloney/2009/06/04/Taxol stabilization of mitotic spindle microtubules - analysis using calcium induced depolymerization
- Categories: Kinesin and microtubule papers, Kinesin and microtubules
- Description: Paper review. Taxol stabilization of mitotic spindle microtubules: analysis using calcium-induced depolymerization
- User:Andy Maloney/Notebook/Lab Notebook of Andy Maloney/2009/06/25/Liposomes and kinesin
- Categories: AM Engineering, Kinesin and microtubules, Surface passivation
- Description: Doing calculations on the amount of lipids needed to coat a cover slip. The idea was to use lipids as the passivation for the kinesin and microtubule experiments.
- User:Andy Maloney/Notebook/Lab Notebook of Andy Maloney/2009/07/31/Metal chelate chromatography
- Categories: Kinesin and microtubule basics, Kinesin and microtubules
- Description: Learning a little bit about protein purification.
- User:Andy Maloney/Notebook/Lab Notebook of Andy Maloney/2009/08/04/Beginning attempts at surface passivation
- Categories: Kinesin and microtubules, Surface passivation
- Description: Beginning attempts at passivating surfaces with lipids. Also, my ideas with spin coatings.
- User:Andy Maloney/Notebook/Lab Notebook of Andy Maloney/2009/08/05/Microscope setup
- Categories: AM Engineering, Kinesin and microtubules
- Description: Issues with visualizing microtubules due to improper installment of the Hg lamp.
- User:Andy Maloney/Notebook/Lab Notebook of Andy Maloney/2009/08/05/Motility experiment
- Categories: Kinesin and microtubules
- Description: My first success at a gliding motility assay.
- User:Andy Maloney/Notebook/Lab Notebook of Andy Maloney/2009/08/06/Kiney computer setup
- Categories: AM Engineering, Kinesin and microtubules
- Description: Talking about the computer we got from surplus for the kinesin experiments.
- User:Andy Maloney/Notebook/Lab Notebook of Andy Maloney/2009/08/07/Spin Coater 3000
- Categories: AM Engineering, Kinesin and microtubules
- Description: Making of the Spin Coater 3000 from my Spin Master Micro.
- User:Andy Maloney/Notebook/Lab Notebook of Andy Maloney/2009/08/10/Gliding motility assay
- Categories: Kinesin and microtubules
- Description: The first movie of a gliding motility assay from KochLab.
- User:Andy Maloney/Notebook/Lab Notebook of Andy Maloney/2009/08/11/Spin coater 3000 Trial 1
- Categories: Kinesin and microtubules, Surface passivation
- Description: Using the Spin Coater 3000.
- User:Andy Maloney/Notebook/Lab Notebook of Andy Maloney/2009/08/12/Surface passivation trial 2
- Categories: Kinesin and microtubules, Surface passivation
- Description: Using the Spin Coater 3000 again. While I was able to coat the slips with lipids, I was never able to get a motility assay going.
- User:Andy Maloney/Notebook/Lab Notebook of Andy Maloney/2009/08/17/Surface passivation
- Categories: Kinesin and microtubules, Surface passivation
- Description: My Spin Coater 3000 didn't cut the mustard. So, we tried using the Fisher centrifuge to coat slips. We were able to do it but, not in a very effective manner.
- User:Andy Maloney/Notebook/Lab Notebook of Andy Maloney/2009/08/19/Surface passivation experiment
- Categories: AM Engineering, Kinesin and microtubules, Surface passivation
- Description: Talking about the whole casein issue and how it doesn't go into solution very easily. I did heat up the solution for the first time and started to question why no one has done this before.
- User:Andy Maloney/Notebook/Lab Notebook of Andy Maloney/2009/08/20/Continued setup for surface passivation
- Categories: Kinesin and microtubules, Surface passivation
- Description: Attempting different concentrations of rhodamine labeled tubulin for fluorescent GMA. Also, I found a webpage and paper that talks about casein in poop. This stemmed from Koch's comment about my heating of PEM to get whole casein to go into solution.
- User:Andy Maloney/Notebook/Lab Notebook of Andy Maloney/2009/08/27/Kinesin walking
- Categories: Kinesin and microtubules
- Description: Attempt to work with Larry on simulating kinesin walking.
- User:Andy Maloney/Notebook/Lab Notebook of Andy Maloney/2009/08/27/Tubulin resuspension
- Categories: Kinesin and microtubules, Surface passivation
- Description: Using kappa casein as the surface passivator. This is before I realized that it doesn't work that well.
- User:Andy Maloney/Notebook/Lab Notebook of Andy Maloney/2009/08/28/Motility assay
- Categories: Kinesin and microtubules, Surface passivation
- Description: My definition for a new naming convention for the buffers used in our experiments. Also, direct confirmation of Hancock's paper about the length of microtubules supported with different bovine caseins.
- User:Andy Maloney/Notebook/Lab Notebook of Andy Maloney/2009/08/31/Passivation studies
- Categories: Kinesin and microtubules, Surface passivation
- Description: Noting my update to the protocol section of KochLab. Also, I lament about my paleness and how overexposure to the sun affects my mental faculties.
- User:Andy Maloney/Notebook/Lab Notebook of Andy Maloney/2009/09/01/Kinesin Motor Mechanics: Binding, Stepping, Tracking, Gating, and Limping Review
- Categories: Kinesin and microtubule papers, Kinesin and microtubules, Kinetic Monte Carlo for Kinesin Processivity
- Description: Paper review. Kinesin Motor Mechanics: Binding, Stepping, Tracking, Gating, and Limping
- User:Andy Maloney/Notebook/Lab Notebook of Andy Maloney/2009/09/02/Surface passivation studies
- Categories: Kinesin and microtubules, Surface passivation
- Description: Question the arose from passivation with kappa casein and with lipids. The lipids don't work and the kappa casein does (sort of as I'll find out later).
- User:Andy Maloney/Notebook/Lab Notebook of Andy Maloney/2009/09/02/Kinesin walking
- Categories: Kinesin and microtubules, Kinetic Monte Carlo for Kinesin Processivity
- Description: More on the simulation of kinesin walking.
- User:Andy Maloney/Notebook/Lab Notebook of Andy Maloney/2009/09/08/Surface passivation studies
- Categories: Kinesin and microtubules, Surface passivation
- Description: Thoughts on surface passivation and the differences between hydrophobic/hydrophilic surfaces. I also used potato starch as a surface passivator for some reason.
- User:Andy Maloney/Notebook/Lab Notebook of Andy Maloney/2009/09/09/Surface passivation studies
- Categories: Kinesin and microtubules, Surface passivation
- Description: More thoughts on if you need to passivate both surfaces of a flow cell. Also, I observed what I called "clumping" of microtubules. In fact, these were Taxol crystals masquerading as microtubule clumps. I won't figure that out till later.
- User:Andy Maloney/Notebook/Lab Notebook of Andy Maloney/2009/09/10/Koch mini meeting
- Categories: Kinesin and microtubules, Kochlab meetings
- Description: Meeting with Koch. Discussed slide cleaning, aliquotting, and making our own buffers.
- User:Andy Maloney/Notebook/Lab Notebook of Andy Maloney/2009/09/10/Surface passivation studies
- Categories: Kinesin and microtubules, Surface passivation
- Description: Using different cleaning methods to produce flow cells resulted in no motility. I believed the reason there was no motility was due to bad kinesin.
- User:Andy Maloney/Notebook/Lab Notebook of Andy Maloney/2009/09/11/Surface passivation studies
- Categories: Kinesin and microtubules, Surface passivation
- Description: Slide cleaning and its affects on motility. All experiments stopped working. In hindsight, it was due to a contamination issue.
- User:Andy Maloney/Notebook/Lab Notebook of Andy Maloney/2009/09/14/Getting motility to work again
- Categories: Kinesin and microtubules, Surface passivation
- Description: Still trying to get motility to work again. I bought all new stuff and made all new solutions. This is a reoccurring theme with these assays.
- User:Andy Maloney/Notebook/Lab Notebook of Andy Maloney/2009/09/21/The chemistry of kinesin and microtubule buffers
- Categories: AM Engineering, Kinesin and microtubule basics, Kinesin and microtubules
- Description: Precursor page to my kinesin and microtubule procedure page. Talks about cleaning, the chemicals used in the assay and the buffers used.
- User:Andy Maloney/Notebook/Lab Notebook of Andy Maloney/2009/09/23/Thoughts on the simulation
- Categories: Kinesin and microtubules, Kinetic Monte Carlo for Kinesin Processivity
- Description: More kinesin simulation ideas.
- User:Andy Maloney/Notebook/Lab Notebook of Andy Maloney/2009/09/29/New stuff
- Categories: AM Engineering, Andy's stock solutions, Kinesin and microtubules
- Description: Received new chemicals to start making new solutions.
- User:Andy Maloney/Notebook/Lab Notebook of Andy Maloney/2009/09/30/Preparing for experiments
- Categories: AM Engineering, Andy's stock solutions, Kinesin and microtubules
- Description: Making new ATP aliquots. I didn't label my tubes in the pic but I eventually did since you should label everything in the lab.
- User:Andy Maloney/Notebook/Lab Notebook of Andy Maloney/2009/10/01/Motility
- Categories: Kinesin and microtubules
- Description: First success at the GMA after figuring out that our pipettors were contaminated.
- User:Andy Maloney/Notebook/Lab Notebook of Andy Maloney/2009/10/03/The osmotic pressure of concentrated solutions and the laws of the perfect solution
- Categories: Kinesin and microtubules
- Description: Paper review. The osmotic pressure of concentrated solutions, and the laws of the perfect solution
- User:Andy Maloney/Notebook/Lab Notebook of Andy Maloney/2009/10/03/Surface passivation
- Categories: Kinesin and microtubules, Surface passivation
- Description: More surface passivation stuff. This time with silica nanoparticles.
- User:Andy Maloney/Notebook/Lab Notebook of Andy Maloney/2009/10/04/2010 Biophysical Society Abstract
- Categories: Kinesin and microtubules
- Description: Koch's 2010 Biophysics abstract corrections for my poster. Also, this page uses Google Docs.
- User:Andy Maloney/Notebook/Lab Notebook of Andy Maloney/2009/10/13/Lipid preps
- Categories: Kinesin and microtubules, Surface passivation
- Description: Surface passivation with lipids still not working.
- User:Andy Maloney/Notebook/Lab Notebook of Andy Maloney/2009/10/14/Lipid surface passivation
- Categories: Kinesin and microtubules, Surface passivation
- Description: Studies with lipids. I will point out that the lipid surfaces do make microtubules stick similar to poly-L-lysine. But, the antifade is just rotten when using a lipid surface.
- User:Andy Maloney/Notebook/Lab Notebook of Andy Maloney/2009/10/15/Lipid passivation
- Categories: Kinesin and microtubules, Surface passivation
- Description: More lipid stuff.
- User:Andy Maloney/Notebook/Lab Notebook of Andy Maloney/2009/10/16/Lipid passivation
- Categories: Kinesin and microtubules, Surface passivation
- Description: My first realization that Taxol solutions should not sit around for very long.
- User:Andy Maloney/Notebook/Lab Notebook of Andy Maloney/2009/10/19/Lipids discussion
- Categories: Kinesin and microtubules, Surface passivation
- Description: Some jibba jabba on lipids.
- User:Andy Maloney/Notebook/Lab Notebook of Andy Maloney/2009/10/20/Heavy water
- Categories: AM D2O, AM Engineering, Kinesin and microtubules
- Description: Preparation of Heavy-PEM.
- User:Andy Maloney/Notebook/Lab Notebook of Andy Maloney/2009/10/21/Heavy PEM
- Categories: AM D2O, Kinesin and microtubules
- Description: First attempts with Heavy-PEM. But, I'm having problems with the kappa casein passivation.
- User:Andy Maloney/Notebook/Lab Notebook of Andy Maloney/2009/10/23/Motility
- Categories: Kinesin and microtubules
- Description: Getting motility working. Of course I now know that it is because of the kappa casein passivation.
- User:Andy Maloney/Notebook/Lab Notebook of Andy Maloney/2009/10/28/Lipid comparisons
- Categories: Kinesin and microtubules, Surface passivation
- Description: Success with motility experiments. Not so much success with lipid trials.
- User:Andy Maloney/Notebook/Lab Notebook of Andy Maloney/2009/10/29/Lipid stuff
- Categories: AM Engineering, Kinesin and microtubules, Surface passivation
- Description: Lamenting on lipid solubility and my procedures for getting them into solution. Also, more computer issues with the microscope setup.
- User:Andy Maloney/Notebook/Lab Notebook of Andy Maloney/2009/10/30/Heavy motility
- Categories: AM D2O, Kinesin and microtubules
- Description: First real experiments using D2O in my PEM solution.
- User:Andy Maloney/Notebook/Lab Notebook of Andy Maloney/2009/10/31/This is insane
- Categories: AM D2O, Kinesin and microtubules
- Description: I got motility in a solution of D2O one day later. Tests with and without AF in Heavy-PEM.
- User:Andy Maloney/Notebook/Lab Notebook of Andy Maloney/2009/11/02/More osmotic pressure studies
- Categories: AM D2O, Kinesin and microtubules
- Description: Showing microtubules last in D2O for a long period of time. Showed that MTs will polymerize in D2O.
- User:Andy Maloney/Notebook/Lab Notebook of Andy Maloney/2009/11/03/Microtubule studies
- Categories: AM D2O, Kinesin and microtubules
- Description: More thoughts on measuring things in Heavy-PEM.
- User:Andy Maloney/Notebook/Lab Notebook of Andy Maloney/2009/11/14
- Categories: AM Engineering, Kinesin and microtubules
- Description: Posters for the DTRA meeting.
- User:Andy Maloney/Notebook/Lab Notebook of Andy Maloney/2009/11/16/Dallas movies
- Categories: Kinesin and microtubules
- Description: Movies to show people at the DTRA meeting.
- User:Andy Maloney/Notebook/Lab Notebook of Andy Maloney/2009/11/25/Sucrose
- Categories: AM OsmoticStress, Kinesin and microtubules
- Description: Using sucrose as an osmolyte in the gliding motility assays.
- User:Andy Maloney/Notebook/Lab Notebook of Andy Maloney/2009/12/01/Taxol Crystals Can Masquerade as Stabilized Microtubules
- Categories: Kinesin and microtubule papers, Kinesin and microtubules
- Description: Paper review. Taxol Crystals Can Masquerade as Stabilized Microtubules
- User:Andy Maloney/Notebook/Lab Notebook of Andy Maloney/2009/12/01/Tubulin Dimers Oligomerize before Their Incorporation into Microtubules
- Categories: Kinesin and microtubule papers, Kinesin and microtubules
- Description: Paper review. Tubulin Dimers Oligomerize before Their Incorporation into Microtubules
- User:Andy Maloney/Notebook/Lab Notebook of Andy Maloney/2009/12/07/Effect of Sodium Chloride on the Solubility of Caseins
- Categories: Kinesin and microtubule papers, Kinesin and microtubules
- Description: Paper review. Effect of Sodium Chloride on the Solubility of Caseins
- User:Andy Maloney/Notebook/Lab Notebook of Andy Maloney/2009/12/08/50 Point
- Categories: Heavy water K&MTs, Kinesin and microtubules, Surface passivation
- Description: Comparison of whole casein and kappa casein passivation.
- User:Andy Maloney/Notebook/Lab Notebook of Andy Maloney/2009/12/09/50 D2O
- Categories: AM D2O, Heavy water K&MTs, Kinesin and microtubules
- Description: Investigating the unusual drop in the 50% D2O data.
- User:Andy Maloney/Notebook/Lab Notebook of Andy Maloney/2009/12/29
- Categories: AM Engineering, Kinesin and microtubules
- Description: Looking at how median filtering works. We ultimately don't use this technique anymore for our data analysis.
- User:Andy Maloney/Notebook/Lab Notebook of Andy Maloney/2010/01/07/Surface passivation idea
- Categories: Kinesin and microtubules, Surface passivation
- Description: Really starting to look at caseins. Abandoning lipids and looking at sialic acids.
- User:Andy Maloney/Kinesin & Microtubule Page/Osmotic stress/Analysis of the gliding fishtailing and circling motions of native microtubules
- Categories: Kinesin and microtubule papers, Kinesin and microtubules
- Description: Paper review. Analysis of the gliding, fishtailing and circling motions of native microtubules.
- User:Andy Maloney/Notebook/Lab Notebook of Andy Maloney/2010/01/20/Casein constituents
- Categories: AM Engineering, Kinesin and microtubules, Surface passivation
- Description: Received casein constituents in today and made 0.5mg/mL PEM solutions with them in it.
- User:Andy Maloney/Notebook/Lab Notebook of Andy Maloney/2010/01/26/Caseins
- Categories: Kinesin and microtubules, Surface passivation
- Description: First experiment to compare the different constituents of bovine casein as a surface passivator.
- User:Andy Maloney/Notebook/Lab Notebook of Andy Maloney/2010/01/28
- Categories: Kinesin and microtubules
- Description: Jotting down ideas and references.
- User:Andy Maloney/Kinesin & Microtubule Page/Microtubule papers/Kinesin follows the microtubules protofilament axis
- Categories: Kinesin and microtubule basics, Kinesin and microtubule papers, Kinesin and microtubules
- Description: Paper review. Kinesin follows the microtubule's protofilament axis
- User:Andy Maloney/Notebook/Lab Notebook of Andy Maloney/2010/02/04
- Categories: Kinesin and microtubules
- Description: First data with using whole casein. The data shows a huge spread in speed.
- User:Andy Maloney/Notebook/Lab Notebook of Andy Maloney/2010/02/05
- Categories: Kinesin and microtubules
- Description: Looking at a motility assay for an extended period of time.
- User:Andy Maloney/Notebook/Lab Notebook of Andy Maloney/2010/02/12
- Categories: Kinesin and microtubules
- Description: Seeing more odd speed increases over the time course of an assay. Still don't know what it is. Using whole casein as the surface passivator.
- User:Andy Maloney/Notebook/Lab Notebook of Andy Maloney/2010/03/04/MT speed and passivation
- Categories: Kinesin and microtubules, Surface passivation
- Description: Looking at all the different casein solutions. I believe that at this time I was still putting casein in my motility solution. I do not do this anymore.
- User:Andy Maloney/Notebook/Lab Notebook of Andy Maloney/2010/03/08/Fixed microtubules
- Categories: AM Engineering, Kinesin and microtubules
- Description: Trying to use poly-L-lysine to fix microtubules to the glass slides. Also looking at speckling on microtubules due to where the rhodamine labels are when I polymerize them.
- User:Andy Maloney/Notebook/Lab Notebook of Andy Maloney/2010/03/17/ReAutomation
- Categories: Kinesin and microtubules, Surface passivation
- Description: First look at gliding motility speeds using the different bovine caseins as passivators. Still no temperature stabilization for this data.
- User:Andy Maloney/Notebook/Lab Notebook of Andy Maloney/2010/03/17/Poly-L-lysine
- Categories: Kinesin and microtubules
- Description: Using poly-L-lysine on the glass surface to fix microtubules.
- User:Andy Maloney/Notebook/Lab Notebook of Andy Maloney/2010/03/26/Dr Dill meeting
- Categories: AM OsmoticStress, Kinesin and microtubules
- Description: Notes from our meeting with Ken Dill.
- User:Andy Maloney/Notebook/Lab Notebook of Andy Maloney/2010/04/01/Casein assay 2
- Categories: Kinesin and microtubules, Surface passivation
- Description: Redoing the casein assay from 2010-03-04.
- User:Andy Maloney/Notebook/Lab Notebook of Andy Maloney/2010/04/01/Osmotic stress planning
- Categories: AM OsmoticStress, Kinesin and microtubules
- Description: This notebook entry plans for using betaine as an osmotic stessor and I note preliminary results.
- User:Andy Maloney/Notebook/Lab Notebook of Andy Maloney/2010/04/02/Osmotic stress mapping
- Categories: AM OsmoticStress, Kinesin and microtubules
- Description: This notebook entry is basically repeating April 1st entry in regards to writing again what I want to do with the osmotic stress experiment.
- User:Andy Maloney/Notebook/Lab Notebook of Andy Maloney/2010/04/02/Heavy water mapping
- Categories: AM D2O, AM HeavyWater, Kinesin and microtubules
- Description: Jotting down ideas for the heavy water experiment.
- User:Andy Maloney/Notebook/Lab Notebook of Andy Maloney/2010/04/02/Updated casein data
- Categories: Kinesin and microtubules, Surface passivation
- Description: This page shows some updated casein data.
- User:Andy Maloney/Notebook/Lab Notebook of Andy Maloney/2010/04/13
- Categories: Kinesin and microtubules
- Description: Looking at data using histograms.
- User:Andy Maloney/Notebook/Lab Notebook of Andy Maloney/2010/05/24/Objective temperature control
- Categories: AM Surface passivation paper, AM Temp effects on kinesin velocity, Kinesin and microtubules
- Description: Mapping out what I should do about stabilizing the temperature of the objective. Also, this page describes my initial study on temperature affects.
- User:Andy Maloney/Notebook/Lab Notebook of Andy Maloney/2010/05/25/More on kinesin speed effects due to temperature
- Categories: AM Surface passivation paper, AM Temp effects on kinesin velocity, Kinesin and microtubules
- Description: his is a follow up to the initial study of temperature issues.
- User:Andy Maloney/Notebook/Lab Notebook of Andy Maloney/2010/05/25/Speeding up kinesin-driven microtubule gliding in vitro by variation of cofactor composition and physicochemical parameters
- User:Andy Maloney/Notebook/Lab Notebook of Andy Maloney/2010/05/25/LED illumination for video-enhanced DIC imaging of single microtubules
- Categories: AM Temp effects on kinesin velocity, Kinesin and microtubule papers, Kinesin and microtubules
- Description: Paper review. LED illumination for video-enhanced DIC imaging of single microtubules
- User:Andy Maloney/Notebook/Lab Notebook of Andy Maloney/2010/05/27/Casein data revisited
- Categories: AM Surface passivation paper, AM Temp effects on kinesin velocity, Kinesin and microtubules, Surface passivation
- Description: Reanalyzing the casein data I took to incorporate the times I took the data at. This shows a temperature affect going on.
- User:Andy Maloney/Notebook/Lab Notebook of Andy Maloney/2010/06/07/LED for microscope temperature control
- Categories: AM Surface passivation paper, AM Temp effects on kinesin velocity, Kinesin and microtubules
- Description: This is my first look at how the Thorlabs LED controller works. The goal is to replace the existing LED with another LED so that I can get a broad spectrum of colors in the microscope.
- User:Andy Maloney/Notebook/Lab Notebook of Andy Maloney/2010/06/17/Temperature stabilization
- Categories: AM Surface passivation paper, AM Temp effects on kinesin velocity, Kinesin and microtubules
- Description: Objective temperature stabilization build, slide warmer, and LED discussions.
- User:Andy Maloney/Notebook/Lab Notebook of Andy Maloney/2010/06/23/Temperature data
- Categories: AM Surface passivation paper, AM Temp effects on kinesin velocity, Kinesin and microtubules
- Description: Posting data with temperature stabilization as well as looking more at the LED from Bridgelux. Also, an experiment where I look at only one spot in the sample.
- User:Andy Maloney/Notebook/Lab Notebook of Andy Maloney/2010/06/25/LED and Spot test
- Categories: AM Surface passivation paper, Kinesin and microtubules
- Description: Data from the other day where I looked at GMA with an LED and continued illumination with the Hg lamp.
- User:Andy Maloney/Notebook/Lab Notebook of Andy Maloney/2010/06/29/Whole casein with temperature stabilization
- Categories: AM Surface passivation paper, AM Temp effects on kinesin velocity, Kinesin and microtubules
- Description: I now have repeated experiments where I have stabilized the temperature of the objective and use whole casein as the passivation.
- User:Andy Maloney/Notebook/Lab Notebook of Andy Maloney/2010/07/19
- Categories: AM Surface passivation paper, AM Temp effects on kinesin velocity, Kinesin and microtubules
- Description: Beginning to notice that my casein solutions are no longer working. All I changed was the temperature stabilization.
- User:Andy Maloney/Notebook/Lab Notebook of Andy Maloney/2010/07/20
- Categories: AM Engineering, AM Surface passivation paper, Kinesin and microtubules
- Description: Making new solutions.
- User:Andy Maloney/Notebook/Lab Notebook of Andy Maloney/2010/07/26/Notebook Entries
- Categories: AM Engineering, Kinesin and microtubules
- Description: Friendfeed thread talking about notebook entries
- User:Andy Maloney/Notebook/Lab Notebook of Andy Maloney/2010/07/26/Whole casein failure
- Categories: AM Engineering, AM SurfacePassivationMTs&Kinesin, AM Surface passivation paper, Kinesin and microtubule basics, Kinesin and microtubules
- Description: Making W-PEM and the failures that I encountered making it.
- User:Andy Maloney/Notebook/Lab Notebook of Andy Maloney/2010/07/26/Microscope repair
- Categories: AM Engineering, AM Surface passivation paper, Kinesin and microtubules, Optical tweezers
- Description: Repairing an IX71 microscope.
- User:Andy Maloney/Notebook/Lab Notebook of Andy Maloney/2010/07/27/Reboot
- Categories: AM Surface passivation paper, Kinesin and microtubules, Surface passivation
- Description: Initial test to see if all my new solutions work. No data.
- User:Andy Maloney/Notebook/Lab Notebook of Andy Maloney/2010/07/28/Updated reboot
- Categories: Kinesin and microtubules, Surface passivation
- Description: Data from yesterday's reboot. Looking at all new solutions. Whole casein speed data.
- User:Andy Maloney/Notebook/Lab Notebook of Andy Maloney/2010/07/28/Caseins
- Categories: AM Surface passivation paper, Kinesin and microtubules, Surface passivation
- Description: Looking at casein passivation with all the new solutions made. Beta, alpha, whole so far. Not looking so good.
- User:Andy Maloney/Notebook/Lab Notebook of Andy Maloney/2010/07/29/Beta casein test
- Categories: AM Surface passivation paper, Kinesin and microtubules, Surface passivation
- Description: Trying the beta casein assay without putting it in the freezer and without filtering it and not putting it in the freezer. Guess what, it was the filtering.
- User:Andy Maloney/Notebook/Lab Notebook of Andy Maloney/2010/08/03/Temperature probe
- Categories: AM Engineering, AM Surface passivation paper, Kinesin and microtubules
- Description: Getting the temperature probe working for the temperature stabilized objective.
- User:Andy Maloney/Notebook/Lab Notebook of Andy Maloney/2010/08/04/Solutions
- Categories: AM Engineering, AM Surface passivation paper, Kinesin and microtubules, Surface passivation
- Description: Again with the remaking of buffers and solutions.
- User:Andy Maloney/Notebook/Lab Notebook of Andy Maloney/2010/08/05/Solutions
- Categories: AM Surface passivation paper, Kinesin and microtubules, Surface passivation
- Description: Finished with remaking solutions.
- User:Andy Maloney/Notebook/Lab Notebook of Andy Maloney/2010/08/05/Casein experiments
- Categories: AM Surface passivation paper, Kinesin and microtubules, Surface passivation
- Description: Noting that the temperature I have the objective at is not hot enough to have a stable speed measurement.
- User:Andy Maloney/Notebook/Lab Notebook of Andy Maloney/2009/05/11/Kinesin and microtubule questions
- Categories: Kinesin and microtubule basics, Kinesin and microtubule papers, Kinesin and microtubules
- Description: Questions about kinesin and microtubules.
- User:Andy Maloney/Notebook/Lab Notebook of Andy Maloney/2010/08/06/Temperature stabilization
- Categories: AM Engineering, AM Surface passivation paper, Kinesin and microtubules
- Description: Epoxied the second thermistor to the objective since the oil used kept seeping under the tape.
- User:Andy Maloney/Notebook/Lab Notebook of Andy Maloney/2010/08/06/Casein studies
- Categories: AM Surface passivation paper, Kinesin and microtubules, Surface passivation
- Description: More casein studies with temperature stabilization. The beta casein assay is still acting "funny", or so I think.
- User:Andy Maloney/Notebook/Lab Notebook of Andy Maloney/2010/08/07/Passivation study
- Categories: AM Surface passivation paper, Kinesin and microtubules, Surface passivation
- Description: More surface passivation studies. I've figured out that the harder it is for a casein constituent to go into solution, the better it acts as a surface passivator. Plus, whole and alpha casein work great. Beta and kappa, not so much. Whole and alpha have motility at the center of the flow cell, beta and kappa has it only near the tape.
- User:Andy Maloney/Notebook/Lab Notebook of Andy Maloney/2010/08/10/Casein study
- Categories: AM Surface passivation paper, Kinesin and microtubules, Surface passivation
- Description: Continued experiments using the different caseins as surface passivators.
- User:Andy Maloney/Notebook/Lab Notebook of Andy Maloney/2010/08/11/More caseins
- Categories: AM Engineering, AM Surface passivation paper, Kinesin and microtubules, Surface passivation
- Description: Taking the last bits of data for the bovine casein comparison experiment.
- User:Andy Maloney/Notebook/Lab Notebook of Andy Maloney/2010/08/12/Casein study
- Categories: AM Surface passivation paper, Kinesin and microtubules, Surface passivation
- Description: Continuing the data taking for my passivation study. I've noticed that kappa and beta casein don't work very well when incubated at room temp. So, I'm going to try the cold thing again.
- User:Andy Maloney/Notebook/Lab Notebook of Andy Maloney/2010/08/13/More casein data taking
- Categories: AM Engineering, AM Surface passivation paper, Kinesin and microtubules, Surface passivation
- Description: Getting the last data points for the passivation study. Also, dealing with the virtualbox update. And, making a stage micrometer.
- User:Andy Maloney/Notebook/Lab Notebook of Andy Maloney/2010/08/26/Group meeting
- Categories: AM Engineering, Kinesin and microtubules
- Description: Group meeting.
- User:Andy Maloney/Notebook/Lab Notebook of Andy Maloney/2010/09/07/Casein data
- Categories: AM Surface passivation paper, Kinesin and microtubules, Surface passivation
- Description: Looking at the movies I've made for the passivation study. I'm going to try an catalog everything I see in them.
- User:Andy Maloney/Notebook/Lab Notebook of Andy Maloney/2010/09/17/Gliding motility assay movie
- Categories: AM Engineering, Kinesin and microtubules, Surface passivation
- Description: Short cartoon of the gliding motiltiy assay.
- User:Andy Maloney/Notebook/Lab Notebook of Andy Maloney/2010/09/21/D2O experiments
- Categories: AM Deuterium effects on kinesin and microtubules, Kinesin and microtubules
- Description: Preparation of stock solutions for heavy hydrogen PEM (H-PEM) and deuterium depleted PEM (DD-PEM). Also, some experiments.
- User:Andy Maloney/Notebook/Lab Notebook of Andy Maloney/2010/09/22/Deuterium effects
- Categories: AM Deuterium effects on kinesin and microtubules, Kinesin and microtubules
- Description: More experiments with deuterium.
- User:Andy Maloney/Notebook/Lab Notebook of Andy Maloney/2010/10/04
- Categories: AM Engineering, Kinesin and microtubules
- Description: Working with Nadia about slide cleaning and the kinesin page. Also, looking at how to automate the tobacco seed experiment.
- User:Andy Maloney/Notebook/Lab Notebook of Andy Maloney/2010/10/05/Preliminary water isotope data
- Categories: AM Water isotope study, Kinesin and microtubules
- Description: Looking at the data I did for initial experiments using water isotopes.
- User:Andy Maloney/Notebook/Lab Notebook of Andy Maloney/2010/10/11/Water isotope experiment
- Categories: AM Water isotope study, Kinesin and microtubules
- Description: Second day doing the water isotope experiments.
- User:Andy Maloney/Notebook/Lab Notebook of Andy Maloney/2010/10/12/Water isotope study
- Categories: AM Engineering, AM Water isotope study, Kinesin and microtubules
- Description: Continuing the water isotope studies.
- User:Andy Maloney/Notebook/Lab Notebook of Andy Maloney/2010/10/19/Water isotope study
- Categories: AM Water isotope study, Kinesin and microtubules
- Description: Continuation of the water isotope study. With a pretty amazing screw up that has prevented polymerization of the microtubules.
- User:Andy Maloney/Notebook/Lab Notebook of Andy Maloney/2010/10/21/New tubulin
- Categories: AM Water isotope study, Kinesin and microtubules
- Description: The new tubulin is in and I suspend it
- User:Andy Maloney/Notebook/Lab Notebook of Andy Maloney/2010/10/23
- Categories: AM Surface passivation paper, Kinesin and microtubules, Surface passivation
- Description: Today I tagged all my notebook entries that pertain to the surface passivation paper.
- User:Andy Maloney/Notebook/Lab Notebook of Andy Maloney/2010/11/02/Water isotope study
- Categories: AM Water isotope study, Kinesin and microtubules
- Description: More water isotope experiments. Plus, experiments where I had to make more O-PEM.
- User:Andy Maloney/Notebook/Lab Notebook of Andy Maloney/2010/11/03/Water isotope study
- Categories: AM Water isotope study, Kinesin and microtubules
- Description: More heavy oxygen water isotope studies.
- User:Andy Maloney/Notebook/Lab Notebook of Andy Maloney/2010/11/05/Water isotope study
- Categories: AM Water isotope study, Kinesin and microtubules
- Description: More water isotope assays. This is the ones with H-PEM.
- User:Andy Maloney/Notebook/Lab Notebook of Andy Maloney/2010/11/09/Water isotope study
- Categories: AM Water isotope study, Kinesin and microtubules
- Description: More data taking.
- User:Andy Maloney/Notebook/Lab Notebook of Andy Maloney/2010/11/10/Water isotope study
- Categories: AM Water isotope study, Kinesin and microtubules
- Description: More data taking.
- User:Andy Maloney/Notebook/Lab Notebook of Andy Maloney/2010/11/11/Water isotope study
- Categories: AM Water isotope study, Kinesin and microtubules
- Description: More data taking for the water isotope study.
- User:Andy Maloney/Notebook/Lab Notebook of Andy Maloney/2011/01/20/Reusable flow cell
- Categories: AM Water isotope study, Kinesin and microtubules
- Description: Looking at how to make a reusable flow cell.
- User:Andy Maloney/Notebook/Lab Notebook of Andy Maloney/2011/01/21/New flow cell
- Categories: AM Engineering, AM Water isotope study, Kinesin and microtubules
- Description: Continued looking into the flow cell that can exchange fluid for more than one experiment.
- User:Andy Maloney/Notebook/Lab Notebook of Andy Maloney/2011/01/24/Reusable flow cell
- Categories: AM Engineering, AM Water isotope study, Kinesin and microtubules
- Description: Mapping what to do with the new reusable flow cell.
- User:Andy Maloney/Notebook/Lab Notebook of Andy Maloney/2011/01/25/Reusable flow cell
- Categories: AM Engineering, AM Water isotope study, Kinesin and microtubules
- Description: Testing the reusable flow cell.
- User:Andy Maloney/Notebook/Lab Notebook of Andy Maloney/2011/01/26/Resealable flow cell
- Categories: AM Engineering, AM Water isotope study, Kinesin and microtubules
- Description: Showing data about the resealable flow cell.
- User:Andy Maloney/Notebook/Lab Notebook of Andy Maloney/2011/01/27/Heavy water storage
- Categories: AM Heavy water storage, Kinesin and microtubules
- Description: Starting the observation of proteins in heavy water storage.
- User:Andy Maloney/Notebook/Lab Notebook of Andy Maloney/2011/01/28/Resealable flow cell
- Categories: AM Engineering, AM Water isotope study, Kinesin and microtubules
- Description: Another round of data taking with the resealable flow cell.
- User:Andy Maloney/Notebook/Lab Notebook of Andy Maloney/2011/02/08/Water isotope experiment
- Categories: AM Water isotope study, Kinesin and microtubules
- Description: Planning what needs to be done for the water isotope experiment.
- User:Andy Maloney/Notebook/Lab Notebook of Andy Maloney/2011/02/10/New stock solutions
- Categories: AM Engineering, AM Water isotope study, Kinesin and microtubules
- Description: Making new stock solutions