User:Andy Maloney/Notebook/Lab Notebook of Andy Maloney/2010/09/21/D2O experiments

Stock solutions

Yesterday and today I prepared stock solutions for heavy hydrogen PEM and deuterium depleted PEM. The procedure/recipe for both of these are below.

Heavy Hydrogen PEM

I am going to call this H-PEM to indicate that it is made up of heavy hydrogen water or, D₂O. I didn't call it D-PEM for reasons below.

• Preparation of a 10x solution of H-PEM
  • 800 mM PIPES
  • 10 mM EGTA
  • 10 mM MgCl₂
  • 1.3544 M NaOH
  • pH 7.30 [[Steve Koch 00:12, 27 August 2011 (EDT): NOTE!!! I am thinking that this is a mistake and the buffer should have been made to 6.48 !!! See: http://friendfeed.com/anthonysalvagno/dbc577ae/preliminary-tobacco-seed-growth-results-those]
  • Filtered through a 0.2 um filter and aliquoted in 2 mL screw top cryogenic vials in the 4C fridge.

Deuterium depleted PEM

This is called DD-PEM.

• • 800 mM PIPES
  • 10 mM EGTA
  • 10 mM MgCl₂
  • 1.2265 M NaOH
  • pH 6.89
  • Filtered through a 0.2 um filter and aliquoted in 2 mL screw top cryogenic vials in the 4C fridge.