User:Andy Maloney/Notebook/Lab Notebook of Andy Maloney/2010/07/19
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I've noticed that my beta casein assays are no longer working. I'm not sure what is going on since my whole casein assays work just fine.
In order to eliminate all possible causes of problems, I've gone ahead and reordered more alpha, beta, and kappa casein. I'm also going to prepare a new solution of PEM. Except now what I plan on doing is making a 10x solution of PEM. I will then aliquot it into convenient quantities and store them in the freezer. I have absolutely no clue if there is gunk in my stock solution of PEM or not and this is the cause of the problems.
So far, I know the following: