# User:Andy Maloney/Notebook/Lab Notebook of Andy Maloney/2010/05/24/Objective temperature control

## Kinesin speed up discussions

So my data shows that kinesin speeds up over time when I do my experiments as can be seen in the below image.

Error creating thumbnail: File with dimensions greater than 12.5 MP

After speaking with both Stefan Diez at BPS 2010 and Erik Schäffer at the TKMM 2010 meeting I'm still not totally convinced that I am witnessing a temperature affect. But, I did look into temperature control.

## Temperature tests

The first test I am going to run is rather simple and was suggested by Koch already.

• Prepare a sample and take data immediately.
• After 10 minutes of data, remove the slide from the microscope.
• Let the slide sit on the bench for 30 minutes and return to the microscope for another 10 minutes of data taking.

I will try this with alpha casein and see what I get.

Andy Maloney 00:54, 25 May 2010 (EDT): Update

Error creating thumbnail: File with dimensions greater than 12.5 MP

So above are the results. This is quite interesting. Indeed there is a temperature affect from the heating of the slide due to the Hg lamp being attached to the microscope. This would suggest that we do indeed need to purchase some sort of temperature stabilization for the objective.

However, I keep seeing this really slow beginning speed when I use the alpha casein. It has happened in all of the experiments I have run using this passivation. There is something there, I just don't know what.

I would also like to point out that Erik and Stefan were correct, there is a temperature affect going on so, thanks again to those guys for the conversations!