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{{#form: Category 1: {{#input: type = select | name = cat1 | value = {{#request:cat1}} | *Protocol

  • MIT:Sequencing BioBrick DNA
  • Agarose gel electrophoresis
  • Preparing chemically competent cells
  • Electrocompetent cells
  • SortoStat/Operation
  • Design a Microfluidic Chip
  • Endy:Pouring plates
  • Agarose Pads for Microscopy
  • DNA ligation
  • Endy:Northern Blot, 32P End-Labeled Probes
  • RNA extraction
  • High Efficiency Transformation
  • Yeast DNA Prep
  • Yeast Colony PCR
  • RNA In Vitro Standards
  • Probe Prep, 32P End-Labeled Probes
  • Endy:RNase Protection Assay
  • Endy:Templiphi
  • Ethanol precipitation of small DNA fragments
  • Purification of DNA
  • Knight:Micropure EZ and Microcon purification
  • Annealing primers
  • PNK Treatment of DNA Ends
  • Miniprep
  • Order a Microfluidic Chip from the Foundry
  • Designing primers
  • Cell Cycle Arrest
  • Phosphatase treatment of linearized vector
  • Electroporation
  • Knight:Annealing and primer extension with Klenow polymerase
  • Studier Lysate Prep
  • Cesium Chloride Purification of T7
  • Colony PCR
  • Pulse-chase protein production
  • Fixing cells
  • In vitro transcription with T7 RNA polymerase
  • Beta-galactosidase assay
  • Knight:DNA ligation using NEB Quick Ligation Kit
  • Modeller
  • Knight:Colony PCR
  • Site-directed mutagenesis
  • Restriction digest
  • Endy:Colony PCR
  • Phenol/chloroform extraction
  • Knight:Restriction Digest
  • Acrylamide gels
  • Bacterial cell culture
  • Endy:E. coli Western Blot
  • Endy:STN Chemostat Protocol
  • Sauer:Expression/purification of 35S-Met proteins
  • Endy:RNA extraction
  • Methods to determine the size of an object in microns
  • Layered plates
  • Knight:Site-directed mutagenesis/Single site
  • Sequencing DNA
  • Beta-Galactosidase Assay (A better Miller)
  • PCR
  • Ethanol precipitation of nucleic acids
  • 'Round-the-horn site-directed mutagenesis
  • Silver: PCR
  • Knight:Agarose gel electrophoresis
  • Silver: Lysate for Western
  • Silver: Colony PCR
  • Silver: Time-Lapse Microscopy
  • Silver: Coomassie Stain
  • Silver: Destain
  • Knight:TOPO TA cloning
  • Library Generation
  • Matsudaira:hsCen2 purification
  • Denaturing acrylamide gel purification of nucleic acids
  • Endy:FACS calibration with beads
  • Resuspension of primers
  • Assaying mating
  • Knight:Preparing electrocompetent cells
  • Dropout plates for yeast
  • Keating:Experimental Protocols:HPLC
  • Digital Tools & Resources
  • Miniprep/Qiagen kit
  • Miniprep/Kit-free high-throughput protocol
  • Transforming chemically competent cells
  • Bacterial transformation
  • Endy:Yeast Colony PCR
  • Making a long term stock of bacteria
  • P1vir phage transduction
  • Silver staining
  • Purification of His-tagged proteins
  • FBA
  • MoMA
  • BLAST
  • Free Sulfhydryl Determination
  • Endy:Double stranding oligo libraries
  • Synthetic Biology:BioBricks/Standard FACS protocol
  • Mouse keratinocyte cultures
  • Homology Modelling
  • Endy:FACS protocols
  • Preparing chemically competent cells (Inoue)
  • Transforming chemically competent cells (Inoue)
  • RNase Protection Assay
  • Northern Blot, 32P End-Labeled Probes
  • RNA Half-life
  • One step 'miniprep' method for the isolation of plasmid DNA
  • TOP10 chemically competent cells
  • Cloning
  • Knight:Sequencing DNA
  • Knight:Annealing and primer extension with Taq polymerase
  • Knight:Reconstituting primers
  • DNA Synthesis from Oligos
  • PCR Overlap Extension
  • Kafatos:Minipreps
  • Miniprep/GET buffer
  • Coot and WinCoot for X-ray Crystallography Model Building
  • Endy:Pouring LB plates from prepped media
  • "Core Sample" PCR: Amplifying DNA from agarose gels
  • Antigen retrieval
  • Knight:Purification of His-tagged proteins/Denaturing
  • Knight:NuPAGE electrophoresis
  • Knight:Electroporation
  • Electrophoretic mobility shift assay
  • Protein blot (Western) hub
  • Preserving yeast cultures
  • Knight:pH meter/Calibration
  • Knight:pH meter/Measurement
  • Chromatin Immunoprecipitation
  • Protein motifs
  • Knight:Electrophoretic mobility shift assay
  • Centrifugal filtration/Nucleic acids
  • Plasmapper
  • Knight:Purification of His-tagged proteins
  • Knight:Purification of His-tagged proteins/Native
  • McKinney:TransformByElectroporation
  • Isolation of murine splenocytes
  • Intracellular cytokine staining for flow cytometry (mouse)
  • Knight:Protein solubility
  • Knight:Purification of His-tagged proteins/Denaturing with refolding
  • Belcher/Knight: Electrocompetent Cells
  • Affymetrix One Cycle Eukaryotic Gene Expression Sample Processing
  • RNA Quality Control
  • Affymetrix Target Hybridization
  • Affymetrix Two Cycle Eukaryotic Gene Expression Sample Processing
  • Knight:Centrifuge desalting
  • Knight:Centrifuge desalting/Zeba columns
  • Affymetrix DNA labelling for gene expression arrays
  • Freimoser:Protocols:FastYeastTransformation
  • Protocols/Consensus template
  • Jamesh008:consensus DNA ligation protocol
  • Annealing and primer extension
  • Filamentous fungi genomic DNA isolation
  • Spheroplast Transformation
  • Knight:In vitro transcription
  • RNA electrophoresis
  • Knight:RNA electrophoresis/Denaturing
  • Knight:RNA electrophoresis
  • Knight:RNA electrophoresis/Native
  • Lory:Quick transformant screen
  • TempliPhi
  • Knight:Maxiprep
  • Knight:PhoenIX Maxiprep Kit
  • Etchevers:Extraction ARN francais
  • Seth:Quick Transformant Screen
  • Knight:TempliPhi
  • Knight:NuPAGE electrophoresis/Fast staining
  • Knight:NuPAGE electrophoresis/Slow staining
  • Knight:NuPAGE electrophoresis/Hybrid staining
  • Sauer:RNA Purification from E. coli
  • Sauer:Lysing E. coli with Lysozymes
  • Quantitating nucleic acids
  • Dot Blot
  • Maxiprep of plasmid DNA from E. coli
  • PCR techniques
  • Real-time PCR
  • QRT-PCR/Single tube
  • QRT-PCR
  • Anneal Extend PCR Protocol - for linking 2 pieces of DNA
  • LacZ staining of cells
  • Knight:Purification of MBP-tagged proteins
  • Hop DNA Isolation
  • Recombineering/Lambda red-mediated gene replacement
  • Sauer:Stitching Genes by PCR
  • Streptavidin purification of DNA fragments
  • Alm:Protoplasting
  • Koch Lab:Protocols/Dig-bio PCR
  • Koch Lab:Protocols/Dig-bio PCR/pRL574 4411 PCR
  • Transformation Protocol for Tobacco
  • Mouse tissue lysis for genotyping
  • Transformation Protocol for Arabidopsis
  • Tamoxifen administration to mice
  • NTA chip creation
  • Marek: Freeze-down/Thaw
  • NTA surface preparation
  • DNase Protocol
  • Knight:NuPAGE electrophoresis/Gel drying
  • Streptomyces:Protocols/DNA Digest
  • Streptomyces:Protocols/Conjugation
  • Streptomyces:Protocols/Mini-Maxi Prep
  • Etchevers:ChIP francais
  • Koch Lab:Protocols/Loading rate clamp
  • Knight:DNA Spots
  • DNA Precipitation
  • Streptomyces:Protocols/PCR
  • Streptomyces:Protocols/Spore Prep
  • Streptomyces:Protocols/Transformation by Electroporation
  • Trizol extraction for RNA and DNA
  • Knight:Site-directed mutagenesis/Multi site
  • NanoBio: Making Unilamellar Vesicles
  • NanoBio: Preparation of Supported Bilayer
  • Mesoplasma florum:Genomic DNA
  • Mesoplasma florum:Electroporation
  • Mesoplasma florum:Inverse PCR Transposon location
  • Knight:Miniprep low copy plasmids
  • Alm:Agarose gel electrophoresis
  • Alm:PCR
  • Mesoplasma florum:Transposome construction
  • QRT-PCR/Two tubes
  • Q-PCR
  • Leprevost/Purification of His-tagged proteins/Denaturing
  • Knight:Beta-galactosidase assay
  • Knight:Beta-galactosidase assay/96 well format
  • Koch Lab:Protocols/Microsphere-DNA tethering
  • Low tech flow cell and wicks.JPG
  • Low tech flow cell.JPG
  • Low tech flow cell changing buffer with wick.JPG
  • Wikiomics:Bioinfo tutorial
  • Wikiomics:BLAST
  • Wikiomics:ChIP-chip
  • Wikiomics:Cloning in silico
  • Wikiomics:Crystallography toolbox
  • Wikiomics:Docking toolbox
  • Wikiomics:Exon microarrays
  • Wikiomics:FASTA
  • Wikiomics:Multiple sequence alignment
  • Wikiomics:PDB
  • Wikiomics:Percentage identity
  • Wikiomics:Phylogenetic databases
  • Wikiomics:Protein function prediction
  • Wikiomics:Protein mass spectrometry
  • Wikiomics:Protein volume
  • Wikiomics:PSI-BLAST
  • Wikiomics:RNA databases
  • Wikiomics:RNA gene-finding
  • Wikiomics:RNA homology search
  • Wikiomics:RNA phylogenetics
  • Wikiomics:RNA secondary structure prediction
  • Wikiomics:Sequence motifs
  • Wikiomics:Site Directed Mutagenesis
  • Wikiomics:Staden
  • Wikiomics:Viewing and sharing genome annotations
  • Tregwiki:Collected regulatory elements
  • Knight:DNA ligation using T4 DNA ligase
  • Butlin:Unix for Bioinformatics - advanced tutorial
  • Cloning and sequencing
  • DNA extraction from tissue
  • Wikiomics:Pathway analysis
  • Butlin:Unix for Bioinformatics - basic tutorial
  • NanoBio: PCR
  • Wikiomics:Genome annotation
  • Wikiomics:BLAST tutorial
  • RNA extraction using trizol/tri
  • Mesoplasma florum:restriction enzyme tests
  • Sacks:RAD-seq
  • Mesoplasma florum: Tn5 Transposase
  • ChIP-Chip E. coli
  • Engineering BioBrick vectors from BioBrick parts/DNA ligation
  • Engineering BioBrick vectors from BioBrick parts/Dephosphorylation
  • Engineering BioBrick vectors from BioBrick parts/Restriction digest
  • Engineering BioBrick vectors from BioBrick parts/Colony PCR
  • Knight:Beta-galactosidase assay/Fluorescence
  • Cell counting
  • Cell counting/plating
  • Myers:EGFR IP
  • Myers:synchronization
  • Generating antibodies
  • DNA extraction - Salting Out
  • Arabidopsis gDNA isolation
  • Jacobs:Protocol BCA Total Protein
  • Jacobs:Protocol Adipocyte Differentiation
  • Jacobs:Protocol RNA Agarose Gel
  • Jacobs:Protocol Collagen Gel Contraction
  • Jacobs:Protocol Oil Red O Staining
  • LeBauer:Protocol/Enzyme
  • ISISBio:Protocols/p11 resin preparation
  • Chromatography
  • Synchronization
  • Mammalian cell culture
  • Agarose gel electrophoresis
  • Cloning
  • Human
  • Buffers
  • Alm
  • Jacobs:Protocol Real-Time PCR
  • Myers:ProteinComplexPurification
  • ISISBio:Protocols/Sortase mediated ligation
  • ISISBio:Protocols/Sortase mediated ligation/Small molecule ligation
  • ISISBio:Protocols/Sortase mediated ligation/Solid support ligation
  • ISISBio:Protocols/Sortase mediated ligation/Protein DNA ligation
  • Gautier:TUNEL
  • Micado Tutorial
  • Assembly pcr
  • Cloning Checklist
  • Koch Lab:Protocols/Oligonucleotide Annealing/Duplexes
  • Koch Lab:Protocols/Oligonucleotide Annealing/Hairpins
  • Wikiomics:DNA sequencing
  • Jacobs:Protocol Subculturing 3T3 Fibroblasts
  • Jacobs:Protocol RNA Isolation Using Tri Reagent
  • Koch Lab:Protocols/Unzipping constructs
  • Unzipping construct.jpg
  • Koch Lab:Protocols/Fork unzipping constructs
  • Koch Lab:Protocols/Unzipping constructs/17mer
  • Jacobs:Protocol NuPAGE Electrophoresis and Western Blotting for Proteins
  • Jacobs:Protocol Agarose Formaldehyde Gel Preparation for RNA and Electrophoresis
  • Koch Lab:Protocols/Unzipping constructs/17mer/Anchoring segment
  • Koch Lab:Protocols/Unzipping constructs/17mer/Adapter duplex
  • Koch Lab:Protocols/Unzipping constructs/17mer/Downstream unzipping segment
  • Myers:Proteomics SOP
  • Jacobs:Protocol Actin Staining
  • Jacobs:Protocol Polymerase Chain Reaction (PCR) Set-up and DNA Agarose Gel
  • Jacobs:Protocol Pipetting Exercise
  • Jacobs:Protocol Protein Gel Staining Using Coomassie Blue Protein Stain
  • Jacobs:Protocol Protein Sodium Dodecyl Sulfate Polyacrylamide Gel Electrophoresis (SDS-PAGE) and Coomassie Staining of Polyacrylamide Gel for Protein
  • Jacobs:Protocol Agarose Formaldehyde Gel Preparation for RNA Electrophoresis
  • Jacobs:Protocol Total Protein Isolation Using RIPA Lysis Buffer
  • Jacobs:Protocol Transfecting NIH 3T3s with GFP-Actin
  • Mesoplasma florum:RNA Purification
  • Jacobs:Protocol 10 bp DNA Ladder
  • Jacobs:Protocol Collagen Gel Contraction 2
  • Jacobs:Protocol Total Protein Isolation using RIPA buffer
  • Jacobs:Protocol Total Protein Isolation using RIPA buffer 2
  • Jacobs:Protocol RNA Sample Loading Buffer
  • Jacobs:Protocol Total RNA Isolation using Tri Reagent
  • Wikiomics:RNA splicing
  • Cell and tissue lysis hub
  • Endy:Northern blot, AlkPhos end-labeled probes
  • Knight:Preparing stabs
  • General Cloning Protocol
  • Jacobs:Protocol Loading Mice
  • Jacobs:Protocol Freezing and Thawing Cells
  • Wikiomics:SAGE
  • Wikiomics:Orthologues
  • Jacobs:Protocol OFF (Large Chambers)
  • ISISBio:Protocols/Purification/Tus
  • Wikiomics:Mutation/SNP detection
  • Duffy:LIC Cloning
  • Bioanalyzer
  • RNA blot (Northern)
  • Refolding Proteins
  • Cell cycle analysis
  • Dahlquist:DNA Microarray Protocol
  • Koch Lab:Protocols/Microsphere-DNA tethering/Glass, dig, biotin, microsphere, 4kb DNA/Cleaning glass
  • Size selective DNA precipitation
  • Griffin: Ultimate Immunoprecipitation Guide
  • Griffin:Nuclear Extract Preparation
  • Griffin:Dual Immunofluorescence Cell Staining
  • Griffin:Western Blot Optimization
  • Cell counting/hemacytometer
  • Griffin:Immunohistochemistry Paraffin
  • Griffin:Membrane Stripping and Reprobing
  • Griffin:Antibody Elution Buffers
  • Isolate leydig cells from testes
  • Griffin:In Vitro Kinase Assay
  • Griffin:Antibody Related Solutions & Recipes
  • Griffin:Puromycin Selection
  • Griffin:Isolate Leukocytes from whole blood
  • Griffin:Nested RT-PCR
  • Griffin:shRNA Transfection
  • Addition of 3' A overhangs to PCR products
  • Weiss Lab:DNA labeling
  • Lactobacillus transformation (Serror 2002)
  • Lactobacillus transformation (Kim 2005)
  • Lactobacillus culture
  • Baby cell column
  • Lactobacillus miniprep
  • Lactobacillus planarum miniprep
  • Jasieniuk:Converting codominant to dominant data
  • Affinity purification of antibodies
  • Chromosomal DNA isolation from E. coli
  • SynBioSS
  • Smolke:Protocols/Western
  • Prbbbb:vector pcr
  • PrbbBB:Protocols
  • DIYbio:Notebook/Keiki Gels
  • Prbbbb:fusion assembly v1
  • Prbbbb:fusion biobrick construction v1
  • Smolke:Protocols/Library transformation
  • Huang: Agarose Slides for Imaging
  • Bionumerics
  • PrbbBB:colony pcr v1
  • Fong:Serum bottle anaerobic culture
  • Fong:Bradford protein assay
  • SB
  • In-fusion biobrick assembly
  • Mathies:Valves
  • Mathies:Drilling a Glass Microfluidic Wafer
  • Fong:RNA gel electrophoresis/Denaturing
  • DNA blot (Southern)
  • Koch Lab:Protocols/Kinesin/BRB80 Acid Pipes
  • Koch Lab:Protocols/Kinesin/AMP-PNP Purification
  • Koch Lab:Protocols/Kinesin/DLS Kinesin Aggregation
  • Koch Lab:Protocols/Kinesin/Tubulin resuspension and polymerization
  • Shreffler:CFSE Labeling
  • Shreffler:Pervanadate
  • Prbbbb:dna measurement on plate v1
  • Shreffler:Oxidative Burst
  • Mathies:Mask Making
  • Mathies:Wafers Cleaning and Deposition
  • Mathies:Photolithography
  • Shreffler:Periodate Treatment
  • Shreffler:RTPCR
  • Shreffler:Intra Cellular Staining
  • Shreffler:Plasmid Purification
  • Richard Lab:ADF
  • Richard Lab:ADL
  • Shreffler:Basophil Activation
  • Richard Lab:Agarose Gel Electrophoresis
  • Antibodies
  • Biomass Analysis
  • Restriction Digest: Partial
  • Mathies:RIE
  • Richard Lab:Restriction Digest
  • Polyacrylamide gel electrophoresis
  • Making RNA probes with T7 transcription
  • Richard Lab: NDF
  • Richard Lab:Electroporation of E. coli
  • Richard Lab:Preparing electrocompetent cells
  • Shreffler:BioCSI Basophil Activation
  • Shreffler:Confocal Protocols
  • Prbbbb:small scale expression v1
  • Mathies:Glass Etching
  • Mathies:Metal Deposition
  • Mathies:Metal Etching
  • Mathies:Glass Bonding
  • Shreffler:Milk Program
  • Mathies:Nitride
  • Mathies:Polyacrylamide Derivitization
  • Mathies:Dynamic Coating
  • Mathies:Sieving Matrices
  • Mathies:Separation Buffer
  • Mathies:Simulations
  • Mathies:Introduction to Cell Culture
  • Mathies:Culture in Broth
  • Mathies:Calibration Curves
  • Mathies:E COLI PCR
  • Mathies:Glass Etching in BNC
  • Mathies:RIE in BNC
  • Mathies:Photolithography in BNC
  • Mathies:Drilling in BNC
  • Mathies:Back Door
  • Shreffler:Jurkat Transfection
  • Prbbbb:Coomassi Ethanol stain
  • Prbbbb:inclusion body solubilization v1
  • Shreffler:Basophil tSyk
  • Saponin Lysis of RBCs
  • Paraffin embedding and sectioning
  • Immunofluorescence Microscopy (Pf, live)
  • Immunofluorescence Microscopy (Pf, fixed)
  • Erman's Lab:DNA Miniprep with Alkaline Lysis
  • Acetobacter Xylinum Culture
  • Electro-transformation of Lactobacillus spp.
  • Genomic miniprep/Sigma kit
  • In vitro modification of DNA for L. plantarum
  • Lactobacillus transformation (Berthier 1996)
  • Shreffler:JAX Basophil Activation
  • TE antigen retrieval and immunostaining
  • Shreffler:JAX Tcell studies
  • Kafatos:Making 1kb+ Ladder Stock
  • DNA Seq Prep
  • Cloning Protocol
  • Shreffler:DC Isolation from skin
  • Jasieniuk:Implementation of a method to calculate microsatellite genotype distance in polyploids
  • DNA Quantification
  • Wikiomics:Repeat finding
  • Shreffler:Cisplatin Allergen
  • Protein Purification - Salting Out
  • Mounting medium
  • Nissl staining
  • Prbbbb:in vitro FRET FRB FKBP v1
  • Shreffler:Basophil Sensitization
  • Gibson Assembly
  • DataONE:Protocols/Find GEO reuses
  • DataONE:Protocols/Scopus citation counts from PMIDs
  • PrbbBB:Oligo Annealing
  • Polar Lipid Analysis
  • Rapid plant gDNA extraction
  • Richard Lab:Amplified insert assembly
  • Feruloyl Esterase Protocols
  • Jacobs: In vivo microCT scanning protocol for live mice
  • Wwwblast
  • Jacobs: Protocol Immunostaining for TRPV4 and primary cilia
  • Jacobs: Protocol In vivo microCT scanning protocol for live mice
  • Jacobs: Protocol Preparation of primary osteocytes and MDCK cells for immunostaining
  • Jacobs:Protocol Cell Culture of MDCK cells
  • Amplified insert assembly
  • Beta-galactosidase Screen
  • Lactobacillus chromosomal integration
  • Lactococcus transformation
  • Sex genotyping of mice
  • Shreffler:Eosinophil Isolation
  • InteLAB
  • Muratore:Protocols/PCR/Basic
  • Muratore:Protocols/Digest/NheI
  • Muratore:Protocols/PCR/Quikchange
  • Muratore:Protocols/Digest/SapI

}} Category 2: {{#input: type = select | name = cat2 | value = {{#request:cat2}} | *Protocol

  • MIT:Sequencing BioBrick DNA
  • Agarose gel electrophoresis
  • Preparing chemically competent cells
  • Electrocompetent cells
  • SortoStat/Operation
  • Design a Microfluidic Chip
  • Endy:Pouring plates
  • Agarose Pads for Microscopy
  • DNA ligation
  • Endy:Northern Blot, 32P End-Labeled Probes
  • RNA extraction
  • High Efficiency Transformation
  • Yeast DNA Prep
  • Yeast Colony PCR
  • RNA In Vitro Standards
  • Probe Prep, 32P End-Labeled Probes
  • Endy:RNase Protection Assay
  • Endy:Templiphi
  • Ethanol precipitation of small DNA fragments
  • Purification of DNA
  • Knight:Micropure EZ and Microcon purification
  • Annealing primers
  • PNK Treatment of DNA Ends
  • Miniprep
  • Order a Microfluidic Chip from the Foundry
  • Designing primers
  • Cell Cycle Arrest
  • Phosphatase treatment of linearized vector
  • Electroporation
  • Knight:Annealing and primer extension with Klenow polymerase
  • Studier Lysate Prep
  • Cesium Chloride Purification of T7
  • Colony PCR
  • Pulse-chase protein production
  • Fixing cells
  • In vitro transcription with T7 RNA polymerase
  • Beta-galactosidase assay
  • Knight:DNA ligation using NEB Quick Ligation Kit
  • Modeller
  • Knight:Colony PCR
  • Site-directed mutagenesis
  • Restriction digest
  • Endy:Colony PCR
  • Phenol/chloroform extraction
  • Knight:Restriction Digest
  • Acrylamide gels
  • Bacterial cell culture
  • Endy:E. coli Western Blot
  • Endy:STN Chemostat Protocol
  • Sauer:Expression/purification of 35S-Met proteins
  • Endy:RNA extraction
  • Methods to determine the size of an object in microns
  • Layered plates
  • Knight:Site-directed mutagenesis/Single site
  • Sequencing DNA
  • Beta-Galactosidase Assay (A better Miller)
  • PCR
  • Ethanol precipitation of nucleic acids
  • 'Round-the-horn site-directed mutagenesis
  • Silver: PCR
  • Knight:Agarose gel electrophoresis
  • Silver: Lysate for Western
  • Silver: Colony PCR
  • Silver: Time-Lapse Microscopy
  • Silver: Coomassie Stain
  • Silver: Destain
  • Knight:TOPO TA cloning
  • Library Generation
  • Matsudaira:hsCen2 purification
  • Denaturing acrylamide gel purification of nucleic acids
  • Endy:FACS calibration with beads
  • Resuspension of primers
  • Assaying mating
  • Knight:Preparing electrocompetent cells
  • Dropout plates for yeast
  • Keating:Experimental Protocols:HPLC
  • Digital Tools & Resources
  • Miniprep/Qiagen kit
  • Miniprep/Kit-free high-throughput protocol
  • Transforming chemically competent cells
  • Bacterial transformation
  • Endy:Yeast Colony PCR
  • Making a long term stock of bacteria
  • P1vir phage transduction
  • Silver staining
  • Purification of His-tagged proteins
  • FBA
  • MoMA
  • BLAST
  • Free Sulfhydryl Determination
  • Endy:Double stranding oligo libraries
  • Synthetic Biology:BioBricks/Standard FACS protocol
  • Mouse keratinocyte cultures
  • Homology Modelling
  • Endy:FACS protocols
  • Preparing chemically competent cells (Inoue)
  • Transforming chemically competent cells (Inoue)
  • RNase Protection Assay
  • Northern Blot, 32P End-Labeled Probes
  • RNA Half-life
  • One step 'miniprep' method for the isolation of plasmid DNA
  • TOP10 chemically competent cells
  • Cloning
  • Knight:Sequencing DNA
  • Knight:Annealing and primer extension with Taq polymerase
  • Knight:Reconstituting primers
  • DNA Synthesis from Oligos
  • PCR Overlap Extension
  • Kafatos:Minipreps
  • Miniprep/GET buffer
  • Coot and WinCoot for X-ray Crystallography Model Building
  • Endy:Pouring LB plates from prepped media
  • "Core Sample" PCR: Amplifying DNA from agarose gels
  • Antigen retrieval
  • Knight:Purification of His-tagged proteins/Denaturing
  • Knight:NuPAGE electrophoresis
  • Knight:Electroporation
  • Electrophoretic mobility shift assay
  • Protein blot (Western) hub
  • Preserving yeast cultures
  • Knight:pH meter/Calibration
  • Knight:pH meter/Measurement
  • Chromatin Immunoprecipitation
  • Protein motifs
  • Knight:Electrophoretic mobility shift assay
  • Centrifugal filtration/Nucleic acids
  • Plasmapper
  • Knight:Purification of His-tagged proteins
  • Knight:Purification of His-tagged proteins/Native
  • McKinney:TransformByElectroporation
  • Isolation of murine splenocytes
  • Intracellular cytokine staining for flow cytometry (mouse)
  • Knight:Protein solubility
  • Knight:Purification of His-tagged proteins/Denaturing with refolding
  • Belcher/Knight: Electrocompetent Cells
  • Affymetrix One Cycle Eukaryotic Gene Expression Sample Processing
  • RNA Quality Control
  • Affymetrix Target Hybridization
  • Affymetrix Two Cycle Eukaryotic Gene Expression Sample Processing
  • Knight:Centrifuge desalting
  • Knight:Centrifuge desalting/Zeba columns
  • Affymetrix DNA labelling for gene expression arrays
  • Freimoser:Protocols:FastYeastTransformation
  • Protocols/Consensus template
  • Jamesh008:consensus DNA ligation protocol
  • Annealing and primer extension
  • Filamentous fungi genomic DNA isolation
  • Spheroplast Transformation
  • Knight:In vitro transcription
  • RNA electrophoresis
  • Knight:RNA electrophoresis/Denaturing
  • Knight:RNA electrophoresis
  • Knight:RNA electrophoresis/Native
  • Lory:Quick transformant screen
  • TempliPhi
  • Knight:Maxiprep
  • Knight:PhoenIX Maxiprep Kit
  • Etchevers:Extraction ARN francais
  • Seth:Quick Transformant Screen
  • Knight:TempliPhi
  • Knight:NuPAGE electrophoresis/Fast staining
  • Knight:NuPAGE electrophoresis/Slow staining
  • Knight:NuPAGE electrophoresis/Hybrid staining
  • Sauer:RNA Purification from E. coli
  • Sauer:Lysing E. coli with Lysozymes
  • Quantitating nucleic acids
  • Dot Blot
  • Maxiprep of plasmid DNA from E. coli
  • PCR techniques
  • Real-time PCR
  • QRT-PCR/Single tube
  • QRT-PCR
  • Anneal Extend PCR Protocol - for linking 2 pieces of DNA
  • LacZ staining of cells
  • Knight:Purification of MBP-tagged proteins
  • Hop DNA Isolation
  • Recombineering/Lambda red-mediated gene replacement
  • Sauer:Stitching Genes by PCR
  • Streptavidin purification of DNA fragments
  • Alm:Protoplasting
  • Koch Lab:Protocols/Dig-bio PCR
  • Koch Lab:Protocols/Dig-bio PCR/pRL574 4411 PCR
  • Transformation Protocol for Tobacco
  • Mouse tissue lysis for genotyping
  • Transformation Protocol for Arabidopsis
  • Tamoxifen administration to mice
  • NTA chip creation
  • Marek: Freeze-down/Thaw
  • NTA surface preparation
  • DNase Protocol
  • Knight:NuPAGE electrophoresis/Gel drying
  • Streptomyces:Protocols/DNA Digest
  • Streptomyces:Protocols/Conjugation
  • Streptomyces:Protocols/Mini-Maxi Prep
  • Etchevers:ChIP francais
  • Koch Lab:Protocols/Loading rate clamp
  • Knight:DNA Spots
  • DNA Precipitation
  • Streptomyces:Protocols/PCR
  • Streptomyces:Protocols/Spore Prep
  • Streptomyces:Protocols/Transformation by Electroporation
  • Trizol extraction for RNA and DNA
  • Knight:Site-directed mutagenesis/Multi site
  • NanoBio: Making Unilamellar Vesicles
  • NanoBio: Preparation of Supported Bilayer
  • Mesoplasma florum:Genomic DNA
  • Mesoplasma florum:Electroporation
  • Mesoplasma florum:Inverse PCR Transposon location
  • Knight:Miniprep low copy plasmids
  • Alm:Agarose gel electrophoresis
  • Alm:PCR
  • Mesoplasma florum:Transposome construction
  • QRT-PCR/Two tubes
  • Q-PCR
  • Leprevost/Purification of His-tagged proteins/Denaturing
  • Knight:Beta-galactosidase assay
  • Knight:Beta-galactosidase assay/96 well format
  • Koch Lab:Protocols/Microsphere-DNA tethering
  • Low tech flow cell and wicks.JPG
  • Low tech flow cell.JPG
  • Low tech flow cell changing buffer with wick.JPG
  • Wikiomics:Bioinfo tutorial
  • Wikiomics:BLAST
  • Wikiomics:ChIP-chip
  • Wikiomics:Cloning in silico
  • Wikiomics:Crystallography toolbox
  • Wikiomics:Docking toolbox
  • Wikiomics:Exon microarrays
  • Wikiomics:FASTA
  • Wikiomics:Multiple sequence alignment
  • Wikiomics:PDB
  • Wikiomics:Percentage identity
  • Wikiomics:Phylogenetic databases
  • Wikiomics:Protein function prediction
  • Wikiomics:Protein mass spectrometry
  • Wikiomics:Protein volume
  • Wikiomics:PSI-BLAST
  • Wikiomics:RNA databases
  • Wikiomics:RNA gene-finding
  • Wikiomics:RNA homology search
  • Wikiomics:RNA phylogenetics
  • Wikiomics:RNA secondary structure prediction
  • Wikiomics:Sequence motifs
  • Wikiomics:Site Directed Mutagenesis
  • Wikiomics:Staden
  • Wikiomics:Viewing and sharing genome annotations
  • Tregwiki:Collected regulatory elements
  • Knight:DNA ligation using T4 DNA ligase
  • Butlin:Unix for Bioinformatics - advanced tutorial
  • Cloning and sequencing
  • DNA extraction from tissue
  • Wikiomics:Pathway analysis
  • Butlin:Unix for Bioinformatics - basic tutorial
  • NanoBio: PCR
  • Wikiomics:Genome annotation
  • Wikiomics:BLAST tutorial
  • RNA extraction using trizol/tri
  • Mesoplasma florum:restriction enzyme tests
  • Sacks:RAD-seq
  • Mesoplasma florum: Tn5 Transposase
  • ChIP-Chip E. coli
  • Engineering BioBrick vectors from BioBrick parts/DNA ligation
  • Engineering BioBrick vectors from BioBrick parts/Dephosphorylation
  • Engineering BioBrick vectors from BioBrick parts/Restriction digest
  • Engineering BioBrick vectors from BioBrick parts/Colony PCR
  • Knight:Beta-galactosidase assay/Fluorescence
  • Cell counting
  • Cell counting/plating
  • Myers:EGFR IP
  • Myers:synchronization
  • Generating antibodies
  • DNA extraction - Salting Out
  • Arabidopsis gDNA isolation
  • Jacobs:Protocol BCA Total Protein
  • Jacobs:Protocol Adipocyte Differentiation
  • Jacobs:Protocol RNA Agarose Gel
  • Jacobs:Protocol Collagen Gel Contraction
  • Jacobs:Protocol Oil Red O Staining
  • LeBauer:Protocol/Enzyme
  • ISISBio:Protocols/p11 resin preparation
  • Chromatography
  • Synchronization
  • Mammalian cell culture
  • Agarose gel electrophoresis
  • Cloning
  • Human
  • Buffers
  • Alm
  • Jacobs:Protocol Real-Time PCR
  • Myers:ProteinComplexPurification
  • ISISBio:Protocols/Sortase mediated ligation
  • ISISBio:Protocols/Sortase mediated ligation/Small molecule ligation
  • ISISBio:Protocols/Sortase mediated ligation/Solid support ligation
  • ISISBio:Protocols/Sortase mediated ligation/Protein DNA ligation
  • Gautier:TUNEL
  • Micado Tutorial
  • Assembly pcr
  • Cloning Checklist
  • Koch Lab:Protocols/Oligonucleotide Annealing/Duplexes
  • Koch Lab:Protocols/Oligonucleotide Annealing/Hairpins
  • Wikiomics:DNA sequencing
  • Jacobs:Protocol Subculturing 3T3 Fibroblasts
  • Jacobs:Protocol RNA Isolation Using Tri Reagent
  • Koch Lab:Protocols/Unzipping constructs
  • Unzipping construct.jpg
  • Koch Lab:Protocols/Fork unzipping constructs
  • Koch Lab:Protocols/Unzipping constructs/17mer
  • Jacobs:Protocol NuPAGE Electrophoresis and Western Blotting for Proteins
  • Jacobs:Protocol Agarose Formaldehyde Gel Preparation for RNA and Electrophoresis
  • Koch Lab:Protocols/Unzipping constructs/17mer/Anchoring segment
  • Koch Lab:Protocols/Unzipping constructs/17mer/Adapter duplex
  • Koch Lab:Protocols/Unzipping constructs/17mer/Downstream unzipping segment
  • Myers:Proteomics SOP
  • Jacobs:Protocol Actin Staining
  • Jacobs:Protocol Polymerase Chain Reaction (PCR) Set-up and DNA Agarose Gel
  • Jacobs:Protocol Pipetting Exercise
  • Jacobs:Protocol Protein Gel Staining Using Coomassie Blue Protein Stain
  • Jacobs:Protocol Protein Sodium Dodecyl Sulfate Polyacrylamide Gel Electrophoresis (SDS-PAGE) and Coomassie Staining of Polyacrylamide Gel for Protein
  • Jacobs:Protocol Agarose Formaldehyde Gel Preparation for RNA Electrophoresis
  • Jacobs:Protocol Total Protein Isolation Using RIPA Lysis Buffer
  • Jacobs:Protocol Transfecting NIH 3T3s with GFP-Actin
  • Mesoplasma florum:RNA Purification
  • Jacobs:Protocol 10 bp DNA Ladder
  • Jacobs:Protocol Collagen Gel Contraction 2
  • Jacobs:Protocol Total Protein Isolation using RIPA buffer
  • Jacobs:Protocol Total Protein Isolation using RIPA buffer 2
  • Jacobs:Protocol RNA Sample Loading Buffer
  • Jacobs:Protocol Total RNA Isolation using Tri Reagent
  • Wikiomics:RNA splicing
  • Cell and tissue lysis hub
  • Endy:Northern blot, AlkPhos end-labeled probes
  • Knight:Preparing stabs
  • General Cloning Protocol
  • Jacobs:Protocol Loading Mice
  • Jacobs:Protocol Freezing and Thawing Cells
  • Wikiomics:SAGE
  • Wikiomics:Orthologues
  • Jacobs:Protocol OFF (Large Chambers)
  • ISISBio:Protocols/Purification/Tus
  • Wikiomics:Mutation/SNP detection
  • Duffy:LIC Cloning
  • Bioanalyzer
  • RNA blot (Northern)
  • Refolding Proteins
  • Cell cycle analysis
  • Dahlquist:DNA Microarray Protocol
  • Koch Lab:Protocols/Microsphere-DNA tethering/Glass, dig, biotin, microsphere, 4kb DNA/Cleaning glass
  • Size selective DNA precipitation
  • Griffin: Ultimate Immunoprecipitation Guide
  • Griffin:Nuclear Extract Preparation
  • Griffin:Dual Immunofluorescence Cell Staining
  • Griffin:Western Blot Optimization
  • Cell counting/hemacytometer
  • Griffin:Immunohistochemistry Paraffin
  • Griffin:Membrane Stripping and Reprobing
  • Griffin:Antibody Elution Buffers
  • Isolate leydig cells from testes
  • Griffin:In Vitro Kinase Assay
  • Griffin:Antibody Related Solutions & Recipes
  • Griffin:Puromycin Selection
  • Griffin:Isolate Leukocytes from whole blood
  • Griffin:Nested RT-PCR
  • Griffin:shRNA Transfection
  • Addition of 3' A overhangs to PCR products
  • Weiss Lab:DNA labeling
  • Lactobacillus transformation (Serror 2002)
  • Lactobacillus transformation (Kim 2005)
  • Lactobacillus culture
  • Baby cell column
  • Lactobacillus miniprep
  • Lactobacillus planarum miniprep
  • Jasieniuk:Converting codominant to dominant data
  • Affinity purification of antibodies
  • Chromosomal DNA isolation from E. coli
  • SynBioSS
  • Smolke:Protocols/Western
  • Prbbbb:vector pcr
  • PrbbBB:Protocols
  • DIYbio:Notebook/Keiki Gels
  • Prbbbb:fusion assembly v1
  • Prbbbb:fusion biobrick construction v1
  • Smolke:Protocols/Library transformation
  • Huang: Agarose Slides for Imaging
  • Bionumerics
  • PrbbBB:colony pcr v1
  • Fong:Serum bottle anaerobic culture
  • Fong:Bradford protein assay
  • SB
  • In-fusion biobrick assembly
  • Mathies:Valves
  • Mathies:Drilling a Glass Microfluidic Wafer
  • Fong:RNA gel electrophoresis/Denaturing
  • DNA blot (Southern)
  • Koch Lab:Protocols/Kinesin/BRB80 Acid Pipes
  • Koch Lab:Protocols/Kinesin/AMP-PNP Purification
  • Koch Lab:Protocols/Kinesin/DLS Kinesin Aggregation
  • Koch Lab:Protocols/Kinesin/Tubulin resuspension and polymerization
  • Shreffler:CFSE Labeling
  • Shreffler:Pervanadate
  • Prbbbb:dna measurement on plate v1
  • Shreffler:Oxidative Burst
  • Mathies:Mask Making
  • Mathies:Wafers Cleaning and Deposition
  • Mathies:Photolithography
  • Shreffler:Periodate Treatment
  • Shreffler:RTPCR
  • Shreffler:Intra Cellular Staining
  • Shreffler:Plasmid Purification
  • Richard Lab:ADF
  • Richard Lab:ADL
  • Shreffler:Basophil Activation
  • Richard Lab:Agarose Gel Electrophoresis
  • Antibodies
  • Biomass Analysis
  • Restriction Digest: Partial
  • Mathies:RIE
  • Richard Lab:Restriction Digest
  • Polyacrylamide gel electrophoresis
  • Making RNA probes with T7 transcription
  • Richard Lab: NDF
  • Richard Lab:Electroporation of E. coli
  • Richard Lab:Preparing electrocompetent cells
  • Shreffler:BioCSI Basophil Activation
  • Shreffler:Confocal Protocols
  • Prbbbb:small scale expression v1
  • Mathies:Glass Etching
  • Mathies:Metal Deposition
  • Mathies:Metal Etching
  • Mathies:Glass Bonding
  • Shreffler:Milk Program
  • Mathies:Nitride
  • Mathies:Polyacrylamide Derivitization
  • Mathies:Dynamic Coating
  • Mathies:Sieving Matrices
  • Mathies:Separation Buffer
  • Mathies:Simulations
  • Mathies:Introduction to Cell Culture
  • Mathies:Culture in Broth
  • Mathies:Calibration Curves
  • Mathies:E COLI PCR
  • Mathies:Glass Etching in BNC
  • Mathies:RIE in BNC
  • Mathies:Photolithography in BNC
  • Mathies:Drilling in BNC
  • Mathies:Back Door
  • Shreffler:Jurkat Transfection
  • Prbbbb:Coomassi Ethanol stain
  • Prbbbb:inclusion body solubilization v1
  • Shreffler:Basophil tSyk
  • Saponin Lysis of RBCs
  • Paraffin embedding and sectioning
  • Immunofluorescence Microscopy (Pf, live)
  • Immunofluorescence Microscopy (Pf, fixed)
  • Erman's Lab:DNA Miniprep with Alkaline Lysis
  • Acetobacter Xylinum Culture
  • Electro-transformation of Lactobacillus spp.
  • Genomic miniprep/Sigma kit
  • In vitro modification of DNA for L. plantarum
  • Lactobacillus transformation (Berthier 1996)
  • Shreffler:JAX Basophil Activation
  • TE antigen retrieval and immunostaining
  • Shreffler:JAX Tcell studies
  • Kafatos:Making 1kb+ Ladder Stock
  • DNA Seq Prep
  • Cloning Protocol
  • Shreffler:DC Isolation from skin
  • Jasieniuk:Implementation of a method to calculate microsatellite genotype distance in polyploids
  • DNA Quantification
  • Wikiomics:Repeat finding
  • Shreffler:Cisplatin Allergen
  • Protein Purification - Salting Out
  • Mounting medium
  • Nissl staining
  • Prbbbb:in vitro FRET FRB FKBP v1
  • Shreffler:Basophil Sensitization
  • Gibson Assembly
  • DataONE:Protocols/Find GEO reuses
  • DataONE:Protocols/Scopus citation counts from PMIDs
  • PrbbBB:Oligo Annealing
  • Polar Lipid Analysis
  • Rapid plant gDNA extraction
  • Richard Lab:Amplified insert assembly
  • Feruloyl Esterase Protocols
  • Jacobs: In vivo microCT scanning protocol for live mice
  • Wwwblast
  • Jacobs: Protocol Immunostaining for TRPV4 and primary cilia
  • Jacobs: Protocol In vivo microCT scanning protocol for live mice
  • Jacobs: Protocol Preparation of primary osteocytes and MDCK cells for immunostaining
  • Jacobs:Protocol Cell Culture of MDCK cells
  • Amplified insert assembly
  • Beta-galactosidase Screen
  • Lactobacillus chromosomal integration
  • Lactococcus transformation
  • Sex genotyping of mice
  • Shreffler:Eosinophil Isolation
  • InteLAB
  • Muratore:Protocols/PCR/Basic
  • Muratore:Protocols/Digest/NheI
  • Muratore:Protocols/PCR/Quikchange
  • Muratore:Protocols/Digest/SapI

}} Category 3: {{#input: type = select | name = cat3 | value = {{#request:cat3}} | *Protocol

  • MIT:Sequencing BioBrick DNA
  • Agarose gel electrophoresis
  • Preparing chemically competent cells
  • Electrocompetent cells
  • SortoStat/Operation
  • Design a Microfluidic Chip
  • Endy:Pouring plates
  • Agarose Pads for Microscopy
  • DNA ligation
  • Endy:Northern Blot, 32P End-Labeled Probes
  • RNA extraction
  • High Efficiency Transformation
  • Yeast DNA Prep
  • Yeast Colony PCR
  • RNA In Vitro Standards
  • Probe Prep, 32P End-Labeled Probes
  • Endy:RNase Protection Assay
  • Endy:Templiphi
  • Ethanol precipitation of small DNA fragments
  • Purification of DNA
  • Knight:Micropure EZ and Microcon purification
  • Annealing primers
  • PNK Treatment of DNA Ends
  • Miniprep
  • Order a Microfluidic Chip from the Foundry
  • Designing primers
  • Cell Cycle Arrest
  • Phosphatase treatment of linearized vector
  • Electroporation
  • Knight:Annealing and primer extension with Klenow polymerase
  • Studier Lysate Prep
  • Cesium Chloride Purification of T7
  • Colony PCR
  • Pulse-chase protein production
  • Fixing cells
  • In vitro transcription with T7 RNA polymerase
  • Beta-galactosidase assay
  • Knight:DNA ligation using NEB Quick Ligation Kit
  • Modeller
  • Knight:Colony PCR
  • Site-directed mutagenesis
  • Restriction digest
  • Endy:Colony PCR
  • Phenol/chloroform extraction
  • Knight:Restriction Digest
  • Acrylamide gels
  • Bacterial cell culture
  • Endy:E. coli Western Blot
  • Endy:STN Chemostat Protocol
  • Sauer:Expression/purification of 35S-Met proteins
  • Endy:RNA extraction
  • Methods to determine the size of an object in microns
  • Layered plates
  • Knight:Site-directed mutagenesis/Single site
  • Sequencing DNA
  • Beta-Galactosidase Assay (A better Miller)
  • PCR
  • Ethanol precipitation of nucleic acids
  • 'Round-the-horn site-directed mutagenesis
  • Silver: PCR
  • Knight:Agarose gel electrophoresis
  • Silver: Lysate for Western
  • Silver: Colony PCR
  • Silver: Time-Lapse Microscopy
  • Silver: Coomassie Stain
  • Silver: Destain
  • Knight:TOPO TA cloning
  • Library Generation
  • Matsudaira:hsCen2 purification
  • Denaturing acrylamide gel purification of nucleic acids
  • Endy:FACS calibration with beads
  • Resuspension of primers
  • Assaying mating
  • Knight:Preparing electrocompetent cells
  • Dropout plates for yeast
  • Keating:Experimental Protocols:HPLC
  • Digital Tools & Resources
  • Miniprep/Qiagen kit
  • Miniprep/Kit-free high-throughput protocol
  • Transforming chemically competent cells
  • Bacterial transformation
  • Endy:Yeast Colony PCR
  • Making a long term stock of bacteria
  • P1vir phage transduction
  • Silver staining
  • Purification of His-tagged proteins
  • FBA
  • MoMA
  • BLAST
  • Free Sulfhydryl Determination
  • Endy:Double stranding oligo libraries
  • Synthetic Biology:BioBricks/Standard FACS protocol
  • Mouse keratinocyte cultures
  • Homology Modelling
  • Endy:FACS protocols
  • Preparing chemically competent cells (Inoue)
  • Transforming chemically competent cells (Inoue)
  • RNase Protection Assay
  • Northern Blot, 32P End-Labeled Probes
  • RNA Half-life
  • One step 'miniprep' method for the isolation of plasmid DNA
  • TOP10 chemically competent cells
  • Cloning
  • Knight:Sequencing DNA
  • Knight:Annealing and primer extension with Taq polymerase
  • Knight:Reconstituting primers
  • DNA Synthesis from Oligos
  • PCR Overlap Extension
  • Kafatos:Minipreps
  • Miniprep/GET buffer
  • Coot and WinCoot for X-ray Crystallography Model Building
  • Endy:Pouring LB plates from prepped media
  • "Core Sample" PCR: Amplifying DNA from agarose gels
  • Antigen retrieval
  • Knight:Purification of His-tagged proteins/Denaturing
  • Knight:NuPAGE electrophoresis
  • Knight:Electroporation
  • Electrophoretic mobility shift assay
  • Protein blot (Western) hub
  • Preserving yeast cultures
  • Knight:pH meter/Calibration
  • Knight:pH meter/Measurement
  • Chromatin Immunoprecipitation
  • Protein motifs
  • Knight:Electrophoretic mobility shift assay
  • Centrifugal filtration/Nucleic acids
  • Plasmapper
  • Knight:Purification of His-tagged proteins
  • Knight:Purification of His-tagged proteins/Native
  • McKinney:TransformByElectroporation
  • Isolation of murine splenocytes
  • Intracellular cytokine staining for flow cytometry (mouse)
  • Knight:Protein solubility
  • Knight:Purification of His-tagged proteins/Denaturing with refolding
  • Belcher/Knight: Electrocompetent Cells
  • Affymetrix One Cycle Eukaryotic Gene Expression Sample Processing
  • RNA Quality Control
  • Affymetrix Target Hybridization
  • Affymetrix Two Cycle Eukaryotic Gene Expression Sample Processing
  • Knight:Centrifuge desalting
  • Knight:Centrifuge desalting/Zeba columns
  • Affymetrix DNA labelling for gene expression arrays
  • Freimoser:Protocols:FastYeastTransformation
  • Protocols/Consensus template
  • Jamesh008:consensus DNA ligation protocol
  • Annealing and primer extension
  • Filamentous fungi genomic DNA isolation
  • Spheroplast Transformation
  • Knight:In vitro transcription
  • RNA electrophoresis
  • Knight:RNA electrophoresis/Denaturing
  • Knight:RNA electrophoresis
  • Knight:RNA electrophoresis/Native
  • Lory:Quick transformant screen
  • TempliPhi
  • Knight:Maxiprep
  • Knight:PhoenIX Maxiprep Kit
  • Etchevers:Extraction ARN francais
  • Seth:Quick Transformant Screen
  • Knight:TempliPhi
  • Knight:NuPAGE electrophoresis/Fast staining
  • Knight:NuPAGE electrophoresis/Slow staining
  • Knight:NuPAGE electrophoresis/Hybrid staining
  • Sauer:RNA Purification from E. coli
  • Sauer:Lysing E. coli with Lysozymes
  • Quantitating nucleic acids
  • Dot Blot
  • Maxiprep of plasmid DNA from E. coli
  • PCR techniques
  • Real-time PCR
  • QRT-PCR/Single tube
  • QRT-PCR
  • Anneal Extend PCR Protocol - for linking 2 pieces of DNA
  • LacZ staining of cells
  • Knight:Purification of MBP-tagged proteins
  • Hop DNA Isolation
  • Recombineering/Lambda red-mediated gene replacement
  • Sauer:Stitching Genes by PCR
  • Streptavidin purification of DNA fragments
  • Alm:Protoplasting
  • Koch Lab:Protocols/Dig-bio PCR
  • Koch Lab:Protocols/Dig-bio PCR/pRL574 4411 PCR
  • Transformation Protocol for Tobacco
  • Mouse tissue lysis for genotyping
  • Transformation Protocol for Arabidopsis
  • Tamoxifen administration to mice
  • NTA chip creation
  • Marek: Freeze-down/Thaw
  • NTA surface preparation
  • DNase Protocol
  • Knight:NuPAGE electrophoresis/Gel drying
  • Streptomyces:Protocols/DNA Digest
  • Streptomyces:Protocols/Conjugation
  • Streptomyces:Protocols/Mini-Maxi Prep
  • Etchevers:ChIP francais
  • Koch Lab:Protocols/Loading rate clamp
  • Knight:DNA Spots
  • DNA Precipitation
  • Streptomyces:Protocols/PCR
  • Streptomyces:Protocols/Spore Prep
  • Streptomyces:Protocols/Transformation by Electroporation
  • Trizol extraction for RNA and DNA
  • Knight:Site-directed mutagenesis/Multi site
  • NanoBio: Making Unilamellar Vesicles
  • NanoBio: Preparation of Supported Bilayer
  • Mesoplasma florum:Genomic DNA
  • Mesoplasma florum:Electroporation
  • Mesoplasma florum:Inverse PCR Transposon location
  • Knight:Miniprep low copy plasmids
  • Alm:Agarose gel electrophoresis
  • Alm:PCR
  • Mesoplasma florum:Transposome construction
  • QRT-PCR/Two tubes
  • Q-PCR
  • Leprevost/Purification of His-tagged proteins/Denaturing
  • Knight:Beta-galactosidase assay
  • Knight:Beta-galactosidase assay/96 well format
  • Koch Lab:Protocols/Microsphere-DNA tethering
  • Low tech flow cell and wicks.JPG
  • Low tech flow cell.JPG
  • Low tech flow cell changing buffer with wick.JPG
  • Wikiomics:Bioinfo tutorial
  • Wikiomics:BLAST
  • Wikiomics:ChIP-chip
  • Wikiomics:Cloning in silico
  • Wikiomics:Crystallography toolbox
  • Wikiomics:Docking toolbox
  • Wikiomics:Exon microarrays
  • Wikiomics:FASTA
  • Wikiomics:Multiple sequence alignment
  • Wikiomics:PDB
  • Wikiomics:Percentage identity
  • Wikiomics:Phylogenetic databases
  • Wikiomics:Protein function prediction
  • Wikiomics:Protein mass spectrometry
  • Wikiomics:Protein volume
  • Wikiomics:PSI-BLAST
  • Wikiomics:RNA databases
  • Wikiomics:RNA gene-finding
  • Wikiomics:RNA homology search
  • Wikiomics:RNA phylogenetics
  • Wikiomics:RNA secondary structure prediction
  • Wikiomics:Sequence motifs
  • Wikiomics:Site Directed Mutagenesis
  • Wikiomics:Staden
  • Wikiomics:Viewing and sharing genome annotations
  • Tregwiki:Collected regulatory elements
  • Knight:DNA ligation using T4 DNA ligase
  • Butlin:Unix for Bioinformatics - advanced tutorial
  • Cloning and sequencing
  • DNA extraction from tissue
  • Wikiomics:Pathway analysis
  • Butlin:Unix for Bioinformatics - basic tutorial
  • NanoBio: PCR
  • Wikiomics:Genome annotation
  • Wikiomics:BLAST tutorial
  • RNA extraction using trizol/tri
  • Mesoplasma florum:restriction enzyme tests
  • Sacks:RAD-seq
  • Mesoplasma florum: Tn5 Transposase
  • ChIP-Chip E. coli
  • Engineering BioBrick vectors from BioBrick parts/DNA ligation
  • Engineering BioBrick vectors from BioBrick parts/Dephosphorylation
  • Engineering BioBrick vectors from BioBrick parts/Restriction digest
  • Engineering BioBrick vectors from BioBrick parts/Colony PCR
  • Knight:Beta-galactosidase assay/Fluorescence
  • Cell counting
  • Cell counting/plating
  • Myers:EGFR IP
  • Myers:synchronization
  • Generating antibodies
  • DNA extraction - Salting Out
  • Arabidopsis gDNA isolation
  • Jacobs:Protocol BCA Total Protein
  • Jacobs:Protocol Adipocyte Differentiation
  • Jacobs:Protocol RNA Agarose Gel
  • Jacobs:Protocol Collagen Gel Contraction
  • Jacobs:Protocol Oil Red O Staining
  • LeBauer:Protocol/Enzyme
  • ISISBio:Protocols/p11 resin preparation
  • Chromatography
  • Synchronization
  • Mammalian cell culture
  • Agarose gel electrophoresis
  • Cloning
  • Human
  • Buffers
  • Alm
  • Jacobs:Protocol Real-Time PCR
  • Myers:ProteinComplexPurification
  • ISISBio:Protocols/Sortase mediated ligation
  • ISISBio:Protocols/Sortase mediated ligation/Small molecule ligation
  • ISISBio:Protocols/Sortase mediated ligation/Solid support ligation
  • ISISBio:Protocols/Sortase mediated ligation/Protein DNA ligation
  • Gautier:TUNEL
  • Micado Tutorial
  • Assembly pcr
  • Cloning Checklist
  • Koch Lab:Protocols/Oligonucleotide Annealing/Duplexes
  • Koch Lab:Protocols/Oligonucleotide Annealing/Hairpins
  • Wikiomics:DNA sequencing
  • Jacobs:Protocol Subculturing 3T3 Fibroblasts
  • Jacobs:Protocol RNA Isolation Using Tri Reagent
  • Koch Lab:Protocols/Unzipping constructs
  • Unzipping construct.jpg
  • Koch Lab:Protocols/Fork unzipping constructs
  • Koch Lab:Protocols/Unzipping constructs/17mer
  • Jacobs:Protocol NuPAGE Electrophoresis and Western Blotting for Proteins
  • Jacobs:Protocol Agarose Formaldehyde Gel Preparation for RNA and Electrophoresis
  • Koch Lab:Protocols/Unzipping constructs/17mer/Anchoring segment
  • Koch Lab:Protocols/Unzipping constructs/17mer/Adapter duplex
  • Koch Lab:Protocols/Unzipping constructs/17mer/Downstream unzipping segment
  • Myers:Proteomics SOP
  • Jacobs:Protocol Actin Staining
  • Jacobs:Protocol Polymerase Chain Reaction (PCR) Set-up and DNA Agarose Gel
  • Jacobs:Protocol Pipetting Exercise
  • Jacobs:Protocol Protein Gel Staining Using Coomassie Blue Protein Stain
  • Jacobs:Protocol Protein Sodium Dodecyl Sulfate Polyacrylamide Gel Electrophoresis (SDS-PAGE) and Coomassie Staining of Polyacrylamide Gel for Protein
  • Jacobs:Protocol Agarose Formaldehyde Gel Preparation for RNA Electrophoresis
  • Jacobs:Protocol Total Protein Isolation Using RIPA Lysis Buffer
  • Jacobs:Protocol Transfecting NIH 3T3s with GFP-Actin
  • Mesoplasma florum:RNA Purification
  • Jacobs:Protocol 10 bp DNA Ladder
  • Jacobs:Protocol Collagen Gel Contraction 2
  • Jacobs:Protocol Total Protein Isolation using RIPA buffer
  • Jacobs:Protocol Total Protein Isolation using RIPA buffer 2
  • Jacobs:Protocol RNA Sample Loading Buffer
  • Jacobs:Protocol Total RNA Isolation using Tri Reagent
  • Wikiomics:RNA splicing
  • Cell and tissue lysis hub
  • Endy:Northern blot, AlkPhos end-labeled probes
  • Knight:Preparing stabs
  • General Cloning Protocol
  • Jacobs:Protocol Loading Mice
  • Jacobs:Protocol Freezing and Thawing Cells
  • Wikiomics:SAGE
  • Wikiomics:Orthologues
  • Jacobs:Protocol OFF (Large Chambers)
  • ISISBio:Protocols/Purification/Tus
  • Wikiomics:Mutation/SNP detection
  • Duffy:LIC Cloning
  • Bioanalyzer
  • RNA blot (Northern)
  • Refolding Proteins
  • Cell cycle analysis
  • Dahlquist:DNA Microarray Protocol
  • Koch Lab:Protocols/Microsphere-DNA tethering/Glass, dig, biotin, microsphere, 4kb DNA/Cleaning glass
  • Size selective DNA precipitation
  • Griffin: Ultimate Immunoprecipitation Guide
  • Griffin:Nuclear Extract Preparation
  • Griffin:Dual Immunofluorescence Cell Staining
  • Griffin:Western Blot Optimization
  • Cell counting/hemacytometer
  • Griffin:Immunohistochemistry Paraffin
  • Griffin:Membrane Stripping and Reprobing
  • Griffin:Antibody Elution Buffers
  • Isolate leydig cells from testes
  • Griffin:In Vitro Kinase Assay
  • Griffin:Antibody Related Solutions & Recipes
  • Griffin:Puromycin Selection
  • Griffin:Isolate Leukocytes from whole blood
  • Griffin:Nested RT-PCR
  • Griffin:shRNA Transfection
  • Addition of 3' A overhangs to PCR products
  • Weiss Lab:DNA labeling
  • Lactobacillus transformation (Serror 2002)
  • Lactobacillus transformation (Kim 2005)
  • Lactobacillus culture
  • Baby cell column
  • Lactobacillus miniprep
  • Lactobacillus planarum miniprep
  • Jasieniuk:Converting codominant to dominant data
  • Affinity purification of antibodies
  • Chromosomal DNA isolation from E. coli
  • SynBioSS
  • Smolke:Protocols/Western
  • Prbbbb:vector pcr
  • PrbbBB:Protocols
  • DIYbio:Notebook/Keiki Gels
  • Prbbbb:fusion assembly v1
  • Prbbbb:fusion biobrick construction v1
  • Smolke:Protocols/Library transformation
  • Huang: Agarose Slides for Imaging
  • Bionumerics
  • PrbbBB:colony pcr v1
  • Fong:Serum bottle anaerobic culture
  • Fong:Bradford protein assay
  • SB
  • In-fusion biobrick assembly
  • Mathies:Valves
  • Mathies:Drilling a Glass Microfluidic Wafer
  • Fong:RNA gel electrophoresis/Denaturing
  • DNA blot (Southern)
  • Koch Lab:Protocols/Kinesin/BRB80 Acid Pipes
  • Koch Lab:Protocols/Kinesin/AMP-PNP Purification
  • Koch Lab:Protocols/Kinesin/DLS Kinesin Aggregation
  • Koch Lab:Protocols/Kinesin/Tubulin resuspension and polymerization
  • Shreffler:CFSE Labeling
  • Shreffler:Pervanadate
  • Prbbbb:dna measurement on plate v1
  • Shreffler:Oxidative Burst
  • Mathies:Mask Making
  • Mathies:Wafers Cleaning and Deposition
  • Mathies:Photolithography
  • Shreffler:Periodate Treatment
  • Shreffler:RTPCR
  • Shreffler:Intra Cellular Staining
  • Shreffler:Plasmid Purification
  • Richard Lab:ADF
  • Richard Lab:ADL
  • Shreffler:Basophil Activation
  • Richard Lab:Agarose Gel Electrophoresis
  • Antibodies
  • Biomass Analysis
  • Restriction Digest: Partial
  • Mathies:RIE
  • Richard Lab:Restriction Digest
  • Polyacrylamide gel electrophoresis
  • Making RNA probes with T7 transcription
  • Richard Lab: NDF
  • Richard Lab:Electroporation of E. coli
  • Richard Lab:Preparing electrocompetent cells
  • Shreffler:BioCSI Basophil Activation
  • Shreffler:Confocal Protocols
  • Prbbbb:small scale expression v1
  • Mathies:Glass Etching
  • Mathies:Metal Deposition
  • Mathies:Metal Etching
  • Mathies:Glass Bonding
  • Shreffler:Milk Program
  • Mathies:Nitride
  • Mathies:Polyacrylamide Derivitization
  • Mathies:Dynamic Coating
  • Mathies:Sieving Matrices
  • Mathies:Separation Buffer
  • Mathies:Simulations
  • Mathies:Introduction to Cell Culture
  • Mathies:Culture in Broth
  • Mathies:Calibration Curves
  • Mathies:E COLI PCR
  • Mathies:Glass Etching in BNC
  • Mathies:RIE in BNC
  • Mathies:Photolithography in BNC
  • Mathies:Drilling in BNC
  • Mathies:Back Door
  • Shreffler:Jurkat Transfection
  • Prbbbb:Coomassi Ethanol stain
  • Prbbbb:inclusion body solubilization v1
  • Shreffler:Basophil tSyk
  • Saponin Lysis of RBCs
  • Paraffin embedding and sectioning
  • Immunofluorescence Microscopy (Pf, live)
  • Immunofluorescence Microscopy (Pf, fixed)
  • Erman's Lab:DNA Miniprep with Alkaline Lysis
  • Acetobacter Xylinum Culture
  • Electro-transformation of Lactobacillus spp.
  • Genomic miniprep/Sigma kit
  • In vitro modification of DNA for L. plantarum
  • Lactobacillus transformation (Berthier 1996)
  • Shreffler:JAX Basophil Activation
  • TE antigen retrieval and immunostaining
  • Shreffler:JAX Tcell studies
  • Kafatos:Making 1kb+ Ladder Stock
  • DNA Seq Prep
  • Cloning Protocol
  • Shreffler:DC Isolation from skin
  • Jasieniuk:Implementation of a method to calculate microsatellite genotype distance in polyploids
  • DNA Quantification
  • Wikiomics:Repeat finding
  • Shreffler:Cisplatin Allergen
  • Protein Purification - Salting Out
  • Mounting medium
  • Nissl staining
  • Prbbbb:in vitro FRET FRB FKBP v1
  • Shreffler:Basophil Sensitization
  • Gibson Assembly
  • DataONE:Protocols/Find GEO reuses
  • DataONE:Protocols/Scopus citation counts from PMIDs
  • PrbbBB:Oligo Annealing
  • Polar Lipid Analysis
  • Rapid plant gDNA extraction
  • Richard Lab:Amplified insert assembly
  • Feruloyl Esterase Protocols
  • Jacobs: In vivo microCT scanning protocol for live mice
  • Wwwblast
  • Jacobs: Protocol Immunostaining for TRPV4 and primary cilia
  • Jacobs: Protocol In vivo microCT scanning protocol for live mice
  • Jacobs: Protocol Preparation of primary osteocytes and MDCK cells for immunostaining
  • Jacobs:Protocol Cell Culture of MDCK cells
  • Amplified insert assembly
  • Beta-galactosidase Screen
  • Lactobacillus chromosomal integration
  • Lactococcus transformation
  • Sex genotyping of mice
  • Shreffler:Eosinophil Isolation
  • InteLAB
  • Muratore:Protocols/PCR/Basic
  • Muratore:Protocols/Digest/NheI
  • Muratore:Protocols/PCR/Quikchange
  • Muratore:Protocols/Digest/SapI

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