Lactobacillus miniprep

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A miniprep for Lactobacillus delbruckii (development in progress)


  1. Incubate cells for 30-45 minutes in TES(50mM Tris[pH 7], 20mM EDTA, 0.2 mM sucrose) with 10mg/ml lysozyme and 70U/ml mutanolysin.
  2. Perform normal QIAGEN miniprep
  3. verify extraction of plasmid by sequencing or digesting and running on gel


Please feel free to post comments, questions, or improvements to this protocol. Happy to have your input!

  1. List troubleshooting tips here.
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  3. Anecdotal observations that might be of use to others can also be posted here.

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  • Kelly Drinkwater 15:09, 17 August 2009 (EDT) Here's a protocol for B. subtilis from the Qiagen website. It's the same as a normal miniprep except you add lysozyme with the buffer P1 to 1mg/mL and incubate at 37C for 10 min. There's nothing posted about how it performs.


Relevant papers and books Serror et al, Applied and Environmental Microbiology, 2002


  • Derek Ju (derekju [at]

or instead, discuss this protocol.