Protein blot (Western) hub

The protein blot, also know as Western blot or immunoblot, is one of the most tried and true experiments for antibody-dependent determination of protein expression/presence in a sample. This pages directs you both to the general and lab-specific protocols regarding protein blotting.

General protein blot protocol

• Protein blot (Western)

Western blot protocols by individual labs

• Endy:Tris-Tricine Acrylamide Gels
• Endy:E. coli Western Blot
• Endy: General Western Blot
• Odom:Western Blot
• Sauer:Western blot
• Ketner: Tissue culture viral lysate Western Blot
• Tissue preparation for western blot

See also

• Griffin:Membrane Stripping and Reprobing
• Griffin:Western Blot Optimization

External links

• Western blot on Wikipedia
• Discussion on Western blots on Protocol Online
• Quantifying protein blot (Western) band without expensive software - Photoshop, ImageJ