Muratore:Protocols/Digest/SapI

SapI digest protocol

Tube	sterile H2O	10X NEB4	DNA	SapI (2 kU/mL)
plasmid	30 μL	5 μL	10 μL of NheI digest	5μL
insert	30 μL	5 μL	10 μL of NheI digest	5μL

1. 2h @ 37°C
2. spin-column purify