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Annealing primers can be used as a fast and cheap way to synthesize a short piece of DNA for which you do not have template DNA to PCR from. See part fabrication for other ways to make a part (contains BioBrick specific details).
- Annealing complementary primers<--For pieces of DNA shorter than the limit on primer length.
- Annealing and primer extension<--For pieces of DNA longer than the limit on primer length.