Antibodies

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Drawing of an antibody - heavy chains red, light chains yellow.

Contents

  • 1 General
  • 2 Antibody protocols
    • 2.1 Korey's treasure trove of antibody-related protocols
    • 2.2 General protocols & pages involving antibodies
  • 3 Lab-specific antibody protocols
  • 4 Other related pages

General

  • Griffin:Antibody Basics - Korey's introduction to antibodies
  • Antibody entry in the Wikipedia

Antibody protocols

Korey's treasure trove of antibody-related protocols

  • Antibody Basics
  • Antibody Peptide Neutralization
  • Antibody Protein Coupling (F:P) Ratios
  • Antibody Elution Buffers
  • Antibody Related Solutions & Recipes
  • Antigen Retrieval Technique
  • Chromatin Immunoprecipitation
  • Dual Immunofluorescence Cell Staining
  • Flow Cytometry prep & labeling
  • Immunoprecipitation Guide
  • Immunohistochemistry Paraffin
  • Immunofluorescence Cell Staining
  • Membrane Stripping and Reprobing
  • Western Blotting Guide
  • Western Blot Optimization

General protocols & pages involving antibodies

  • Generating antibodies
  • Protein blot (Western)
  • Dot Blot
  • Affinity purification of antibodies

Lab-specific antibody protocols

  • Hoatlin:Crosslinking antibodies to beads
  • Sauer:Western blot
  • Silver:Immunofluorescence
  • Wittrup: Protein conjugation with fluorophores
  • Silver: FISH/IF
  • Berglund:Antibody Storage

Other related pages

  • Antibodypedia
  • Toolbox lecture including ELISA
Retrieved from "https://openwetware.org/mediawiki/index.php?title=Antibodies&oldid=313909"
Categories:
  • Material
  • Protein
  • Protocol

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  • This page was last edited on 11 June 2009, at 06:21.
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