Lactobacillus culture

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Overview

General overview and guidelines on how to grow up a culture of Lactobacillus

Materials

• MRS broth (difco)
• MRS agar (difco)
• anaerobic conditions

Procedure

1. Follow instructions to make MRS plates and media, autoclave to sterilize
2. Streak Lactobacillus on MRS plate or inoculate into test tube of MRS media
3. Incubate at 37C under anaerobic conditions
4. If from new source of Lactobacillus (ex. dehydrated bacteria from ATCC) allow up to 3 days to grow. Otherwise, allow 12-24 hours to grow

Notes

Please feel free to post comments, questions, or improvements to this protocol. Happy to have your input!

1. For anaerobic conditions, buy gaspak, or for the cheaper solution light a candle inside an airtight box

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Contact

• Derek Ju (derekju [at] mit.edu)

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