Making RNA probes with T7 transcription

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mRNA sense & antisense probes

Probes need to be antisense respective to the mRNA to be able to bind their target. Care has to be taken during experimental design to make a suitable antisense probe.

In vitro T7 transcription of an RNA probe

Designing primers for T7 RNA synthesis

  1. designing a suitable primer combining the T7 promoter sequence and a sequence specific part
  2. attaching a T7 promoter via PCR
  3. in vitro transcription using T7 polymerase and the PCR product as a template

See also

External links