Lactobacillus planarum miniprep

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Overview

Method to miniprep plasmid DNA from Lactobacillus plantarum

Procedure

1. Supplement Qiagen P1 buffer with 5mg/ml lysozyme, 15U/ml mutanolysin, 100ug/ml RNase.
2. incubate at 37°C for 15-30 minutes.
3. Add Qiagen P2 buffer and continue miniprep as usual.
4. Verify plasmid existance by sequencing or running digests on gel

Notes

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References

Relevant papers and books

Mathiesen, G., H. M. Namlos, et al. (2004). "Use of bacteriocin promoters for gene expression in Lactobacillus plantarum C11." Journal of Applied Microbiology 96(4): 819-827.

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