Beta-galactosidase assay

Methods to assay for beta-galactosidase

Specific protocols

• Media:B-gal protocol.doc
• Sauer:beta-Galactosidase Assay (A better Miller) <-- highly recommend
• BE.109:Protein engineering/Assessing beta-galactosidase
• Knight:Beta-galactosidase_assay
• Knight:Beta-galactosidase assay/96 well format
• Knight:Beta-galactosidase assay/Fluorescence
• See also Beta-galactosidase assays in yeast

See also

• Beta-galactosidase Screen
• LacZ staining of cells

References

1. ISBN:0879691069

   original β-galactosidase assay by Miller

2. Griffith KL and Wolf RE Jr. Measuring beta-galactosidase activity in bacteria: cell growth, permeabilization, and enzyme assays in 96-well arrays. Biochem Biophys Res Commun. 2002 Jan 11;290(1):397-402. DOI:10.1006/bbrc.2001.6152 |

   96 well format