Beta-galactosidase assay

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Methods to assay for beta-galactosidase

Specific protocols

  • Media:B-gal protocol.doc
  • Sauer:beta-Galactosidase Assay (A better Miller) <-- highly recommend
  • BE.109:Protein engineering/Assessing beta-galactosidase
  • Knight:Beta-galactosidase_assay
  • Knight:Beta-galactosidase assay/96 well format
  • Knight:Beta-galactosidase assay/Fluorescence
  • See also Beta-galactosidase assays in yeast

See also

  • Beta-galactosidase Screen
  • LacZ staining of cells

References

  1. ISBN:0879691069 [Miller-1972]

    original β-galactosidase assay by Miller

  2. Griffith KL and Wolf RE Jr. Measuring beta-galactosidase activity in bacteria: cell growth, permeabilization, and enzyme assays in 96-well arrays. Biochem Biophys Res Commun. 2002 Jan 11;290(1):397-402. DOI:10.1006/bbrc.2001.6152 | PubMed ID:11779182 | HubMed [Griffith-2002]

    96 well format

Retrieved from "http:///mediawiki/index.php?title=Beta-galactosidase_assay&oldid=500165"
Categories:
  • Protocol
  • In vitro
  • Protein

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  • This page was last edited on 22 March 2011, at 13:10.
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