Colony PCR
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General Information
Colony PCR is can be used after a transformation to screen colonies for the desired plasmid. Primers are used which generate a PCR product of known size. Thus, any colonies which give rise to an amplification product of the expected size are likely to contain the correct DNA sequence.
Specific Protocols
- Endy:Colony PCR
- Knight:Colony PCR
- McClean:_Colony_PCR_(Yeast)
- Silver:_Colony_PCR
- Blackburn:Yeast_Colony_PCR
Notes
- I have compared Taq and Vent using this reaction mix and found that Taq worked better. For very long amplicons, I would recommend using Phusion, which has its own reaction mix. I have not found a difference between Phusion and Taq on amplicons shorter than ~3kb.
