NTA surface preparation

Overview

The purpose of this protocol is to provide a detailed method for the preparation of an NTA chip for the Biacore S51.

Materials

• NTA Sensor chip with an un-used Flow Cell
• Reagent Rack 1
• Small Plastic vials (4) , type BR-1002-12
• 10mM Glycine pH 2.2 (150ul)
• 50mM NaOH (150ul)
• BiaNormalization Solution (200ul)
• 52ug/ml MgCl2 (30 ul)

Procedure

1. Put at least 150ul of 10mM Glycine pH 2.2 in to a small vial and place in the Biacore rack
2. Put at least 150ul of 50mM NaOH into a small vial vial and place in the Biacore rack
3. Create new or re-use a small vial of BiaNormalization Solution and place in into the rack at position F6
4. Eject the rack currently in the Biacore and replace with the rack containing Glycine and NaOH.
5. Start and interactive run with a PBS-P(0.005%) mobile phase
   1. Flow rate = 30
   2. Flow Cell = The new Flow Cell
   3. Spots = 1 & 2
6. Enter the Glycine and NaOH into the solutions table
7. Mark the appropriate settings
   1. Prepare the flow system if necessary
   2. Normalize the detector
   3. Drag the samples to their appropriate locations
8. Preform 3 alternating 1 minute injections (30 ul) of Glycine and NaOH
9. Check the NTA surface by starting another interactive run and inject 52ug/ml MgCl2 for 1 minute at a flow rate of 10 ul/min.

Contact

Talk to Jason Fuller to discuss this protocol.