NTA surface preparation
The purpose of this protocol is to provide a detailed method for the preparation of an NTA chip for the Biacore S51.
- NTA Sensor chip with an un-used Flow Cell
- Reagent Rack 1
- Small Plastic vials (4) , type BR-1002-12
- 10mM Glycine pH 2.2 (150ul)
- 50mM NaOH (150ul)
- BiaNormalization Solution (200ul)
- 52ug/ml MgCl2 (30 ul)
- Put at least 150ul of 10mM Glycine pH 2.2 in to a small vial and place in the Biacore rack
- Put at least 150ul of 50mM NaOH into a small vial vial and place in the Biacore rack
- Create new or re-use a small vial of BiaNormalization Solution and place in into the rack at position F6
- Eject the rack currently in the Biacore and replace with the rack containing Glycine and NaOH.
- Start and interactive run with a PBS-P(0.005%) mobile phase
- Flow rate = 30
- Flow Cell = The new Flow Cell
- Spots = 1 & 2
- Enter the Glycine and NaOH into the solutions table
- Mark the appropriate settings
- Prepare the flow system if necessary
- Normalize the detector
- Drag the samples to their appropriate locations
- Preform 3 alternating 1 minute injections (30 ul) of Glycine and NaOH
- Check the NTA surface by starting another interactive run and inject 52ug/ml MgCl2 for 1 minute at a flow rate of 10 ul/min.
Talk to Jason Fuller to discuss this protocol.