Kafatos:Making 1kb+ Ladder Stock

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Making 1kb+ DNA Ladder Stock. We dilute the entire contents of the tube and make 5x 500uL aliquots


  1. 250uL 1kb+ DNA Ladder [1ug/uL] (Invitrogen)
  2. 500uL 5x DNA Loading Buffer
  3. 1750uL AccuGene Water (Lonza)


  1. Add the 3 components above together
  2. Mix well and make 5 x 500uL aliquots
  3. Leave one working stock at 4C and keep the other frozen at -20C
  4. Use 10uL of this stock per lane (1ug of ladder per lane)


Please feel free to post comments, questions, or improvements to this protocol. Happy to have your input!

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