Griffin:Antibody Elution Buffers
Elution buffers are essential in order to affinity purify antibodies. There are several out there, and below are a representation of 4 very good ones.
- 100mM Glycine, pH2.5 (acidic pH)
- 1M Triethanolamine; TEA (basic pH)
- 4M MgCl2 (high salt)
- 1M NaCl/PBS (high salt)
In general high salt concentration or extreme pH will disrupt the antibody-protein binding. The optimal elution buffer to use will have to be determined experimentally by the user.
Antibody Solution Clean-up
Removing BSA, gelatin, or any other stabilizer protein from antibody stock solutions is a necessary precursor to performing primary amine-based chemical labeling and conjugation procedures (ie Biotin, Fluorescent dye).