Griffin:Antibody Elution Buffers

From OpenWetWare
Jump to navigationJump to search

Elution Buffers

Elution buffers for noncovalent purification.

  • 100mM Glycine, pH2.5 (acidic pH)
  • 1M Triethanolamine; TEA (basic pH)
  • 4M MgCl2 (high salt)
  • 1M NaCl/PBS (high salt)

High salt concentration or extreme pH disrupt hydrostatic (antibody-protein) binding. Optimal elution parameters should be determined experimentally.

Antibody Solution Carrier Protein Removal

Removing BSA, gelatin, or any other stabilizer protein from antibody stock solutions is a necessary precursor to performing primary amine-based chemical labeling and conjugation procedures (ie Biotin, Fluorescent dye).