Griffin:Antibody Elution Buffers
Elution buffers for noncovalent purification.
- 100mM Glycine, pH2.5 (acidic pH)
- 1M Triethanolamine; TEA (basic pH)
- 4M MgCl2 (high salt)
- 1M NaCl/PBS (high salt)
High salt concentration or extreme pH disrupt hydrostatic (antibody-protein) binding. Optimal elution parameters should be determined experimentally.
Antibody Solution Carrier Protein Removal
Removing BSA, gelatin, or any other stabilizer protein from antibody stock solutions is a necessary precursor to performing primary amine-based chemical labeling and conjugation procedures (ie Biotin, Fluorescent dye).