UA Biophysics:Protocols

From OpenWetWare
Jump to navigationJump to search

BioPhysicsUA.jpg


ANTIBIOTICS STOCK

Ampicillin
Kanamycin
Chloramphenicol
Ofloxacin


BUFFERS

PBS
TAE 10X
Calcein
Elution Buffer
K2HPO4 KH2PO4 Buffer

CHEMICALS

KOH
NOH

EXTRACTION

Lipid Extration
Carotenoid Extration

IMMUNOSTAINING

Lymphocytes immunostaining with anti-AlphaTubulin
Tubulin Polymerization with AlexaFluor 488 staining

MEDIA

M9 Salts (5x) 1L
M9 (5x) 1L
LB 1L
LB Plates 1L
M9 Plates
SD 1L
1M MgSO4
0.1M CaCl2
TB 1L

MICROFLUIDICS

Soft lithography
Master fabrication
Plasma Cleaner Protocol
Mother Machine
Microfluidics Valves
Microfluidic System for Biofilm growth
Biofilm growth

MOLECULAR BIOLOGY

Q5 Polymerase
Phusion Polymerase

PLASMIDS AND RELATED

GenEluteTM HP Plasmid Miniprep Kit
Preparing Chemically Competent Cells
Transforming Chemically Competent
Competent Cells
Transformation of Competent Cells
Yeast Miniprep

BACTERIA

Stocks

PHAGES

Marking T4 DNA with DAPI
Direct Plating Plaque Assay
Small Drop Plaque Assay System

C.ELEGANTS

Buffer M9 para C. elegans2
Buffer 1M KPO4 pH 6.02
Cholesterol in ethanol 5 mg/ml2
Nematode Growth Medium (NGM)2
Siembra de nemátodos2
Freezing C. elegans Strains2
Buffer Freezing C. elegans Strains2

VESICLES

Lipid stock
Multilamellar Vesicles
LUV
GUV

TURBIDOSDOSTATS

Plate turbidostat user manual
Revolver turbidostat with interchanging media


Return to UA Biophysics