UA Biophysics:Protocols

ANTIBIOTICS STOCK • Ampicillin • Kanamycin • Chloramphenicol • Erythromycin BUFFERS • PBS • TAE 10X • Calcein • Elution Buffer • KH2PO4 Buffer CHEMICALS • KOH • NOH EXTRACTION • Bacteria for Lipid Extraction • Lipid Extration • Carotenoid Extration IMMUNOSTAINING • Lymphocytes immunostaining with anti-AlphaTubulin • Tubulin Polymerization with AlexaFluor 488 staining MEDIA • M9 Salts (5x) 1L • M9 (5x) 1L • LB 1L • LB Plates 1L • M9 Plates • SD 1L • 1M MgSO4 • 0.1M CaCl2 • TB 1L MICROFLUIDICS • Soft lithography • Master fabrication • Plasma Cleaner Protocol • Mother Machine • Microfluidics Valves • Microfluidic System for Biofilm growth • Biofilm growth MOLECULAR BIOLOGY • Q5 Polymerase • Phusion Polymerase PLASMIDS AND RELATED • GenEluteTM HP Plasmid Miniprep Kit • Competent Cells • Transformation of Competent Cells • Yeast Miniprep BACTERIA • Stocks PHAGES • Marking T4 DNA with DAPI • Direct Plating Plaque Assay • Small Drop Plaque Assay System C.ELEGANTS • Buffer M9 para C. elegans2 • Buffer 1M KPO4 pH 6.02 • Cholesterol in ethanol 5 mg/ml2 • Nematode Growth Medium (NGM)2 • Siembra de nemátodos2 • Freezing C. elegans Strains2 • Buffer Freezing C. elegans Strains2 VESICLES • Lipid stock • Multilamellar Vesicles • LUV • GUV TURBIDOSDOSTATS • Plate turbidostat user manual • Revolver turbidostat with interchanging media Return to UA Biophysics