UA Biophysics:Protocols

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ANTIBIOTICS STOCK

• Ampicillin
• Kanamycin
• Chloramphenicol
• Erythromycin

BUFFERS

• PBS
• TAE 10X
• Calcein
• Elution Buffer
• Buffer HEPES
• KH2PO4 Buffer
• KOH
• NOH

VESICLES

• Lipid stock
• Multilamellar Vesicles
• LUV
• GUV

EXTRACTION

• Lipid Extration
• Carotenoid Extration

MEDIA

• M9 Salts (5x) 1L
• M9 (5x) 1L
• LB 1L
• LB Plates 1L
• M9 Plates
• SD 1L
• 1M MgSO₄
• 0.1M CaCl₂
• TB 1L

MICROFLUIDICS

• Soft lithography
• Master fabrication
• Plasma Cleaner Protocol
• Mother Machine
• Microfluidics Valves
• Microfluidic System for Biofilm growth
• Biofilm growth

MOLECULAR BIOLOGY

• Q5 Polymerase
• Phusion Polymerase
• GenElute^TM HP Plasmid Miniprep Kit
• Competent Cells
• Transformation of Competent Cells
• Yeast Miniprep

IMMUNOSTAINING

• Lymphocytes immunostaining with anti-AlphaTubulin
• Tubulin Polymerization with AlexaFluor 488 staining

BACTERIA

• Stocks
• Bacteria for Lipid Extraction

PHAGES

• Marking T4 DNA with DAPI
• Direct Plating Plaque Assay
• Small Drop Plaque Assay System

C.ELEGANTS

• Buffer M9 para C. elegans₂
• Buffer 1M KPO4 pH 6.0₂
• Cholesterol in ethanol 5 mg/ml₂
• Nematode Growth Medium (NGM)₂
• Siembra de nemátodos₂
• Freezing C. elegans Strains₂
• Buffer Freezing C. elegans Strains₂

TURBIDOSDOSTATS

• Plate turbidostat user manual
• Revolver turbidostat with interchanging media

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