UA Biophysics:Protocols:Lipid Extraction
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Materials
Protocol
- The samples are dissolve in a mixture of chloroform/methanol/water (3:6:1 v/v):
Add chloroform, vortex 5 minutes
Add methanol vortex 5 minutes
Add water vortex 1 minute
and then vortexed every 15min for 4h(solution have to be homogeneous
- The samples are then allowed to stand for 2 days to separate the three phases, after which the lower phase of chloroform and lipid is drawn off by aspiration and collected in a clean glass tube
- A lipid film is formed by drying the chloroform with a gentle stream of N2 and store at -20°C until further experimentation
