UA Biophysics:Protocols:TB 1L
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Terrific broth (TB, not to be confused with T Broth)
Ingredients
- 12 g Tryptone.
- 24 g Yeast extract.
- 4 mL Glycerol.
- 100 mL 0.17M KH2PO4 and 0.72M K2HPO4, sterile, to be prepared separately from the tryptone, yeast extract, and glycerol solution.
Protocol
- Mix tryptone, yeast extract and glycerol, and add distilled water up to 900mL.
- Pour into 1 L flask (or greater).
Autoclave
- Allow liquid to cool to less than 60 degrees Centrigrade.
- Add 100 mL K2HPO4 KH2PO4 Buffer