UA Biophysics:Protocols: Multilamellar Vesicles

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Multilamellar Vesicles The dried lipid, prepared as described in "liposome stock", is hydrated using a hydration buffer.

  1. Preheat the buffer at a temperature approximately 5° above the phase transition temperature of the highest melting lipid component.
  2. Add the buffer to de lipid to achieve the desired lipid concentration.
  3. Vortex the sample during one min, let it stand for 5 min. Repeat 5 times to ensure maximal hydratation