UA Biophysics:Protocols: Multilamellar Vesicles
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Multilamellar Vesicles The dried lipid, prepared as described in "liposome stock", is hydrated using a hydration buffer.
- Preheat the buffer at a temperature approximately 5° above the phase transition temperature of the highest melting lipid component.
- Add the buffer to de lipid to achieve the desired lipid concentration.
- Vortex the sample during one min, let it stand for 5 min. Repeat 5 times to ensure maximal hydratation