UA Biophysics:Protocols:Marking T4 DNA with DAPI

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This protocol CAN'T be done at the installations of the biophysics laboratory as it compromises the safety of the biological samples present at it.


Mark T4 DNA with 4’,6-diamidino-2-phenylindle (DAPI).


For this procedure, a modified version of the methodology given by Furukawa et. al.[1] elaborated for a simple of 10^9 PFU/ μl will be used:

1. E. coli B is cultured to 0.5 x 10^8 cells per ml in 25 ml of TSB 30 gr/l (OXOID).

2. The phage is added ensuring a multiplicity of infection (MOI) of 5 particles per cell.

3. Simultaneously, DAPI (4’,6-diamidino-2-phenylindle from SIGMA) is added to a concentration of 5 μg/ml.

4. 6 hours later cloroform is added to 0.1%.

Other Links

  • Fluorescence Ex/Em spectra of DAPI bound to DNA: [1]


[1] Furukawa, H., Kuroiwa, T. & Mizushima, S. DNA injection during bacteriophage T4 infection of Escherichia coli. J. Bacteriol. 154, 938–945 (1983).


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