Kanamycin disulfate salt from Streptomyces kanamyceticus. Aminoglycoside antibiotic
Kanamycin acts by binding to the 70S ribosomal subunit, inhibiting translocation and eliciting miscoding. Is effective against gram-negative and gram-positive bacteria, and mycoplasma.
Kanamycin is usually used at a concentration of 25-50 µg/mL. Stock solution is typically at 25mg/mL
- According to desired amount of antibiotics, calculate the amount in grams that has to be weighed.
- For example, for 10mL of 25mg/mL of Kanamycin:
- Kanamycin is soluble in ddH2O.
- Filter sterilize solution using a 0.2 µm syringe filter.
- Divide in aliquots of 500μL
- Store at -20 °C. up to 4-6 months
It is best to avoid repeated freeze/thaw cycles of the kanamycin aliquots. Kanamycin is light sensitive. Keep tightly closed in light-resistant containers. "Solutions are stable at 37°C for approximately 5 days. Aqueous stock solutions can be stored at 2-8°C for long term storage", Sigma. Plates with LB can be stored one month.
For culture plates, allow media to cool to 55 °C before adding ampicillin to a final concentration of 35-50 μg/ml.
For Example, for 300 mL of media at a final concentration of 50µg/mL:
Ci Vi = Cf Vf
25mg/mL* Vi = 0,05mg/mL*300mL
Vi = 15mg/(25mg/mL)
Vi = 0,6 mL