UA Biophysics:Protocols:K2HPO4 KH2PO4 Buffer
For the preparation of 1 liter of potassium phosphate solution the final desired concentrations are:
- 0.17 Molar monobasic potassium phospate (23.135 grams/liter KH2PO4, molecular weight=136.09 grams/mole)
- 0.72 Molar dibasic potassium phosphate (125.410 grams/liter K2HPO4, molecular weight=174.18 grams/mole).
Add the dry ingredients to a 1 liter graduated cylinder and added double distilled water until a final volume of 1 liter is reached. Sterile filter this solution through a 0.22 micron filter OR autoclave.