UA Biophysics:Protocols:M9 plates
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M9 plates (20 plates with 20 ml each one)
First step: Mix
- M9 salts 5x 80ml
- Agar 6g
- MilliQ 240 ml
Autoclave and cool to 50C
Second step: Add to the first step
- 20% glucose 4ml (20 g/100 ml MilliQ, filter)
- 1M MgSO4 400 ul (12 g/100 ml MilliQ, filter)
- 0.1M CaCl2 400 ul (1.1 g/100 ml MilliQ, filter)
- Aspartic Acid (1.5968 g/80 ml MilliQ, filter)
Third step: Mix solutions from first and second step and place on petri dishes