DropBase:Devices

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This is an overview of the device designs that are currently available from this repository. If you would like to have your design(s) added to the repository, then please register to edit OpenWetWare and add it to the repository yourself, following the templates of the already available designs. Alternatively, you can email your design (in CAD format) and a brief description to us at the addresses found here.

If you have used a design that you have downloaded from DropBase and would like to contribute some usage notes then please add them in the indicated section of the appropriate page, or email us.

Device modules by function

Droplet generation A variety of different flow-focusing junctions can be found here with varying nozzle sizes and numbers of inlets. 2 inlets devices 3 inlets devices 4 inlets devices

Droplet sorting Fluorescence Activated Droplet Sorters (FADS) FADS device 1 FADS device 2 FADS device 3 FADS device 4 FADS device 5 Absorbance Activated Droplet Sorters (AADS) AADS AADS device 2 Image Activated Droplet Sorters (IADS) IADS

Other Designs Picoinjection Picoinjection device 1 Picoinjection device 2 Extraction Extraction chips High throughput kinetics in droplets Fluidic connector Anchored droplet arrays Anchored droplet

Publications

2024

Microdroplet screening rapidly profiles a biocatalyst to enable its AI-assisted engineering M.Gantz et al, bioRxiv, 2023, DOI:
Pioinjection chip

Selection of a Promiscuous Minimalist cAMP Phosphodiesterase from a Library of De Novo Designed Proteins D.Schnettler et al, bioRxiv, 2023, DOI: 10.1101/2023.02.13.528392 Sorter and FF devices

2023

• Investigating bacteria-phage interaction dynamics using droplet-based technology N. Nikolic et al, bioRxiv, 2023, DOI: 10.1101/2023.07.14.549014
  Anchored droplet array
• Microfluidics-enabled fluorescence-activated cell sorting of single pathogen-specific antibody secreting cells for the rapid discovery of monoclonal antibodies K. Fischer et al, bioRxiv, 2023, DOI: 10.1101/2023.01.10.523494
  Agarose bead generator
• spinDrop: a droplet microfluidic platform to maximise single-cell sequencing information content J. De Jonghe, T. S. Kaminski et al, bioRxiv, 2023, DOI: 10.1101/2023.01.12.523500
  spinDrop sorter
  spinDrop picoinjector
  
• Ultrahigh-throughput directed evolution of a metal-free α/β-hydrolase with a Cys-His-Asp triad into an efficient phosphotriesterase D. Schnettler et al, J Am Chem Soc, 2023, DOI: 10.1021/jacs.2c10673
  FADS device off chip
  FADS device delay line 5 min
  FADS device delay line 30 min
• Versatile Product Detection via Coupled Assays for Ultrahigh-Throughput Screening of Carbohydrate-Active Enzymes in Microfluidic Droplets Ladeveze et al, ACS Catal, 2023 , doi: 10.1021/acscatal.3c01609
  Double FF device (2 inlets)
  Triple FF device (3 inlets)
  Droplet generator (2 inlets)
  Picoinjector
  AADS sorter

2022

• High throughput steady-state enzyme kinetics measured in a parallel droplet generation and absorbance detection platform Neun et al, Anal Chem, 2022, 94(48):16701-16710. doi: 10.1021/acs.analchem.2c03164
  Fluidic connector
• Functional metagenomic screening identifies an unexpected β-glucuronidase Neun et al, Nat. Chem. Biol., 2022, 18(10):1096-1103. doi: 10.1038/s41589-022-01071-x
  Droplet generation (3 inlets)
  Droplet sorting - FADS
• Ultrahigh-throughput Absorbance Activated Droplet Sorting (UHT-AADS) for enzyme screening at kilohertz frequencies. E. J. Medcalf et al., Anal Chem, 2023, 95(10):4597-4604. doi: 10.1021/acs.analchem.2c04144
  UHT-AADS Sorter
  
• High-throughput total RNA sequencing in single cells using VASA-seq F. Salmen, J. De Jonghe et al., Nature Biotechnology, 2022, doi: 10.1038/s41587-022-01361-8.
  VASA_generator
  VASA_1st_picoinjecton
  VASA_2nd_picoinjecton
  

2021

• A Titratable Cell Lysis-on-Demand System for Droplet-Compartmentalised Ultrahigh-Throughput Screening in Functional Metagenomics and Directed Evolution. Wong et al, ACS Synth. Biol, 2021
  Devices for 24 µm double emulsions
• Cell-free Directed Evolution of a Protease in Microdroplets at Ultrahigh Throughput. Holstein et al, ACS Synth. Biol, 2021
  Droplet generation (2 inlets)
  Picoinjection
  Droplet sorting
• The proportion of periportal mesenchyme to ductal epithelial cells acts as a rheostat for liver cell proliferation Cordero-Espinoza et al, CellStemCell, 2021
  Droplet generation (3 inlets)
• Agarose microgel culture delineates lumenogenesis in naive and primed human pluripotent stem cells Schindler et al, Stem Cell Rep., 2021
  Agarose droplet

2020

• Investigating host-microbiome interactions by droplet based microfluidics Tauzin et al, 2020, Microbiome
  Devices for 20 µm double emulsions
  

2019

• Long-Term Perfusion Culture of Monoclonal Embryonic Stem Cells in 3D Hydrogel Beads for Continuous Optical Analysis of Differentiation. Kleine-Brüggeney et al, Small, 2019
  Droplet Generation 70µm
  Extraction Chips (EDC2 & ECD3)
  Trapping Chips (TDC1 & TCD2)
  

2016

• Ultrahigh-throughput–directed enzyme evolution by absorbance-activated droplet sorting (AADS). Gielen et al, PNAS, 2016
  AADS

2012

• Picoliter cell lysate assays in microfluidic droplet compartments for directed enzyme evolution. Kintses et al, Chemistry & Biology, 2012
  3 inlets with short delay channel
  3 inlets with long delay channel