DropBase:Devices

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This is an overview of the device designs that are currently available from this repository. If you would like to have your design(s) added to the repository, then please register to edit OpenWetWare and add it to the repository yourself, following the templates of the already available designs. Alternatively, you can email your design (in CAD format) and a brief description to us at the addresses found here.

If you have used a design that you have downloaded from DropBase and would like to contribute some usage notes then please add them in the indicated section of the appropriate page, or email us.


Device modules by function

Droplet generation

A variety of different flow-focusing junctions can be found here with varying nozzle sizes and numbers of inlets.

2 inlets devices

3 inlets devices

4 inlets devices


Droplet sorting

  • Image Activated Droplet Sorters (IADS)
    IADS

Other Designs





Publications

2024

  • Selection of a Promiscuous Minimalist cAMP Phosphodiesterase from a Library of De Novo Designed Proteins D.Schnettler et al, bioRxiv, 2023, DOI: 10.1101/2023.02.13.528392
    Sorter and FF devices
  • 2023


    2022

    • High throughput steady-state enzyme kinetics measured in a parallel droplet generation and absorbance detection platform Neun et al, Anal Chem, 2022, 94(48):16701-16710. doi: 10.1021/acs.analchem.2c03164
      Fluidic connector
    • Functional metagenomic screening identifies an unexpected β-glucuronidase Neun et al, Nat. Chem. Biol., 2022, 18(10):1096-1103. doi: 10.1038/s41589-022-01071-x
      Droplet generation (3 inlets)
      Droplet sorting - FADS
    • Ultrahigh-throughput Absorbance Activated Droplet Sorting (UHT-AADS) for enzyme screening at kilohertz frequencies. E. J. Medcalf et al., Anal Chem, 2023, 95(10):4597-4604. doi: 10.1021/acs.analchem.2c04144
      UHT-AADS Sorter
    • High-throughput total RNA sequencing in single cells using VASA-seq F. Salmen, J. De Jonghe et al., Nature Biotechnology, 2022, doi: 10.1038/s41587-022-01361-8.
      VASA_generator
      VASA_1st_picoinjecton
      VASA_2nd_picoinjecton


    2021

    • A Titratable Cell Lysis-on-Demand System for Droplet-Compartmentalised Ultrahigh-Throughput Screening in Functional Metagenomics and Directed Evolution. Wong et al, ACS Synth. Biol, 2021
      Devices for 24 µm double emulsions
    • Cell-free Directed Evolution of a Protease in Microdroplets at Ultrahigh Throughput. Holstein et al, ACS Synth. Biol, 2021
      Droplet generation (2 inlets)
      Picoinjection
      Droplet sorting
    • The proportion of periportal mesenchyme to ductal epithelial cells acts as a rheostat for liver cell proliferation Cordero-Espinoza et al, CellStemCell, 2021
      Droplet generation (3 inlets)
    • Agarose microgel culture delineates lumenogenesis in naive and primed human pluripotent stem cells Schindler et al, Stem Cell Rep., 2021
      Agarose droplet

    2020



    2019



    2016


    • Ultrahigh-throughput–directed enzyme evolution by absorbance-activated droplet sorting (AADS). Gielen et al, PNAS, 2016
      AADS


    2012