• Devices to generate 20µm double emulsions or Water-Oil-Water droplets for analysis by Flow Cytometry or sorting by FACS
• The first chip has 3 inlets for (i) Cells (ii)Substrate and (iii) the carrier oil+ surfactant phase
• The second chip has 2 inlets for (i) the first emulsion and (ii) a carrier aqueous phase.
Tauzin, A.S., Pereira, M.R., Van Vliet, L.D. et al. Investigating host-microbiome interactions by droplet based microfluidics. Microbiome 8, 141 (2020). https://doi.org/10.1186/s40168-020-00911-z
Designed by: Martin Fischlechner
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The second chip should be rendered hydrophilic following the procedure detailed in the publication. First chip: flow rates were 1 μL/min total for the aqueous phases (cells and substrate) and 8 μL/min for the carrier oil phase. Seconde chip: start with 1µL/ min for the emulsion and 3µL/min for the aqueous phase. LvV, 2020