DropBase:droplet generation 3 inlets short delay
• Dual aqueous inlet flow-focusing junction of 18 µm width with short delay channel
Kintses B., Hein C, Mohamed MF, Fischlechner M, Courtois F, Lainé C, Hollfelder F, Chemistry & Biology 2012, Picoliter cell lysate assays in microfluidic droplet compartments for directed enzyme evolution, 19(8):1001-9.
Submitted by: Balint Kintses
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With flow rates of aqueous phase 200 µL/h total and oil 1300 µL/h this device gave 35 µm droplets at a throughput of ~6 kHz. SD