Dropbase: Droplet generator

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Overview

Description

• Two aqueous inlets device for generation of 100-200 pL droplets with a flow focusing section of 50 µm
• The height of the channels was 50 µm
• Zig-zag post generation for mixing of droplets
• Allows for mixing 2 aqueous solutions prior to generation
• "Teeths" located at the FF junction reduce jetting and satellites

Reference

Ladeveze et al, Versatile Product Detection via Coupled Assays for Ultrahigh-Throughput Screening of Carbohydrate-Active Enzymes in Microfluidic Droplets, ACS Catal, 2023, doi:0.1021/acscatal.3c01609

Downloads

Two aqueous inlets flow-focussing device for 100 pL droplets: png, dwg

Usage Notes

• Using an oil flow of 18 µL/min together with the aqueous phases at 5 µl/min each, we generated 100 pL droplets @ 1.6kHz. Flow rates of 24µl/min for oil and 2µl/min for the aqueous phases resulted in 50pL droplets @ 1.3 kHz. SL