Dropbase: Droplet generator
From OpenWetWare
Jump to navigationJump to search
![](https://s3-us-west-2.amazonaws.com/oww-files-thumb/c/cd/DropBase_logo.png/150px-DropBase_logo.png)
Overview
![](https://s3-us-west-2.amazonaws.com/oww-files-thumb/f/fa/FF-50x50.png/160px-FF-50x50.png)
Description
• Two aqueous inlets device for generation of 100-200 pL droplets with a flow focusing section of 50 µm
• The height of the channels was 50 µm
• Zig-zag post generation for mixing of droplets
• Allows for mixing 2 aqueous solutions prior to generation
• "Teeths" located at the FF junction reduce jetting and satellites
Reference
Ladeveze et al, Versatile Product Detection via Coupled Assays for Ultrahigh-Throughput Screening of Carbohydrate-Active Enzymes in Microfluidic Droplets, ACS Catal, 2023, doi:0.1021/acscatal.3c01609
Downloads
Usage Notes
- Using an oil flow of 18 µL/min together with the aqueous phases at 5 µl/min each, we generated 100 pL droplets @ 1.6kHz. Flow rates of 24µl/min for oil and 2µl/min for the aqueous phases resulted in 50pL droplets @ 1.3 kHz. SL