DropBase:droplet electrosorting 2

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Overview

Description

• Improved device for electrosorting of droplets into parallel channels. The droplet re-injection channel is 25 μm wide.

Reference

Colin, PY., Kintses, B., Gielen, F. et al. Ultrahigh-throughput discovery of promiscuous enzymes by picodroplet functional metagenomics. Nat Commun 6, 10008 (2015). https://doi.org/10.1038/ncomms10008

Submitted by: Pierre-Yves Colin




Downloads

  1. 25 μm: png, dxf, dwg




Usage Notes

Please enter any comments that other users may find useful below this note (such as flow rates that worked well for particular oil/aqueous phases). When providing usage notes please provide as much detail as useful. We would request that you 'sign' any comments with your initials.

15 μm droplets were re-injected at 30 μL/h into the device and spaced-out using extra oil (2x300 μL/h) and sorted at >2 kHz. Illumination was provided by a laser beam focused on the channel (positioned between the 2 electrodes) with fluorescence detected using a photomultiplier that triggered sorting of positive droplets by application of a square pulse of 0.6-0.8 kV to the electrodes. PYC