DropBase:droplet electrosorting 2
• Improved device for electrosorting of droplets into parallel channels. The droplet re-injection channel is 25 μm wide.
Colin, PY., Kintses, B., Gielen, F. et al. Ultrahigh-throughput discovery of promiscuous enzymes by picodroplet functional metagenomics. Nat Commun 6, 10008 (2015). https://doi.org/10.1038/ncomms10008
Submitted by: Pierre-Yves Colin
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15 μm droplets were re-injected at 30 μL/h into the device and spaced-out using extra oil (2x300 μL/h) and sorted at >2 kHz. Illumination was provided by a laser beam focused on the channel (positioned between the 2 electrodes) with fluorescence detected using a photomultiplier that triggered sorting of positive droplets by application of a square pulse of 0.6-0.8 kV to the electrodes. PYC