• Device for the extraction of 70µm agarose beads from an oil phase into an aqueous (medium) phase.
• The microfluidic chip has three inlets: one for the aqueous phase (PBS or cell culture medium), one for the oil phase containing hydrogel microdroplets, and one for a sheath fluid (HFE-7500 and surfactant) that is used for hydrodynamic focusing and spacing of incoming droplets.
Reference Long-Term Perfusion Culture of Monoclonal Embryonic Stem Cells in 3D Hydrogel Beads for Continuous Optical Analysis of Differentiation. Kleine-Brüggeney, H., van Vliet, L. D., Mulas, C., Gielen, F., Agley, C. C., Silva, J. C. R., Smith, A., Chalut, K., Hollfelder, F., Small 2019, 15, 1804576. https://doi.org/10.1002/smll.201804576
Designed by: Hans Kleine-Brüggeney
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The publication used indium for the electrodes, but we recommend using 5M NaCl instead. The electrodes were used for inducing electrocoalescence between droplets and the aqueous phase by applying an electric AC field (Model 601C, TREK). To increase the stability of the oil/water interface, the channel for the aqueous phase was coated with acrylic acid and the channel for the oil phase was coated with PFOTS (see publication for details). LvV, 2020