• Device for electrosorting of droplets. Channel width is 40 μm.
Kintses B., Hein C, Mohamed MF, Fischlechner M, Courtois F, Lainé C, Hollfelder F, Chemistry & Biology 2012, Picoliter cell lysate assays in microfluidic droplet compartments for directed enzyme evolution, 19(8):1001-9.
Submitted by: Balint Kintses
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35 μm droplets injected at 60 μL/h with oil at 2 x 400 μL/h allowed sorting at ~ 1 kHz. Illumination was provided by a laser beam focused on the channel with fluorescence detected using a photomultiplier that triggered sorting of positive droplets by application of a square pulse of 0.6-0.8 kV to the electrodes. SD