DropBase:TCD

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Overview

Description

• Device for trapping up to 500 hydrogel beads (70µm) for perfusion with an aqueous phase.
• Each device contains 4 lanes of 500 traps.

Reference Long-Term Perfusion Culture of Monoclonal Embryonic Stem Cells in 3D Hydrogel Beads for Continuous Optical Analysis of Differentiation. Kleine-Brüggeney, H., van Vliet, L. D., Mulas, C., Gielen, F., Agley, C. C., Silva, J. C. R., Smith, A., Chalut, K., Hollfelder, F., Small 2019, 15, 1804576. https://doi.org/10.1002/smll.201804576
Designed by: Hans Kleine-Brüggeney

Downloads

Trapping chip TCD1: png, dwg

Trappin chip TCD2: png, dwg

Usage Notes

Please enter any comments that other users may find useful below this note (such as flow rates that worked well for particular oil/aqueous phases). When providing usage notes please provide as much detail as useful. We would request that you 'sign' any comments with your initials.

The 70µm hydrogel beads can be loaded manually or using a syringe pump. The maximum flow of 150 μL/min was used to perfuse the trapped hydrogel beads with media.