• Devices to generate 7 pL double emulsions (~ 20µm) or Water-Oil-Water droplets for analysis by Flow Cytometry or sorting by FACS
• The first chip has 3 inlets for (i) Cells (ii)Substrate and (iii) the carrier oil+ surfactant phase
• The second chip has 2 inlets for (i) the first emulsion and (ii) a carrier aqueous phase.
Wong CFA, van Vliet L, Bhujbal SV, Guo C, Sletmoen M, Stokke BT, Hollfelder F, Lale R. A Titratable Cell Lysis-on-Demand System for Droplet-Compartmentalised Ultrahigh-Throughput Screening in Functional Metagenomics and Directed Evolution. ACS Synthetic Biology (2021). https://doi.org/10.1021/acssynbio.1c00084
Designed by: Tomasz Kaminski, Remkes Scheele and Yanik Weber.
Triple inlet FF - First emulsion: png, dwg
Double inlet FF - Second emulsion: png, dwg
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The second chip should be rendered hydrophilic following the procedure detailed in the publication.
First chip: flow rates were 1 μL/min total for the aqueous phases (cells and substrate) and 8 μL/min for the carrier oil phase.
Second chip: start with 0.5µL/ min for the emulsion and 1µL/min for the aqueous phase, ensuring that the first emulsion is well packed. LvV, 2021