Protocols/Dynamic

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AU-BDL

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Acetobacter xylinum

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Agar Plates

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Agarose gel electrophoresis

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Alm

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Antibody

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Arabidopsis thaliana

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Bacteria

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Bibliometrics

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Biomass Analysis

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Biophysics

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Buffers

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Cell culture

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Chemical

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Chromatography

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Cloning

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DIYbio

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DNA

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  • "Core Sample" PCR: Amplifying DNA from agarose gels
  • 'Round-the-horn site-directed mutagenesis
  • Affymetrix DNA labelling for gene expression arrays
  • Agarose gel electrophoresis
  • Alm:Agarose gel electrophoresis
  • Amplified insert assembly
  • Anneal Extend PCR Protocol - for linking 2 pieces of DNA
  • Annealing and primer extension
  • Annealing primers
  • Arabidopsis gDNA isolation
  • Assembly pcr
  • BLAST
  • Bioanalyzer
  • Butlin:Unix for Bioinformatics - advanced tutorial
  • Butlin:Unix for Bioinformatics - basic tutorial
  • Cell and tissue lysis hub
  • Centrifugal filtration/Nucleic acids
  • Cloning
  • Cloning Checklist
  • Cloning Protocol
  • Cloning and sequencing
  • Colony PCR
  • Corum:DNA Digestion
  • Corum:DNA Hybridization
  • Corum:DNA Quantification
  • Corum:DNA Sequencing
  • Corum:Digestion
  • Corum:Gel Purification
  • Corum:Miniprep
  • Corum:PCR
  • Corum:PCR Purification
  • Corum:Protocols
  • Corum:T4 Ligation
  • Corum:Whole Plasmid PCR
  • Corum:digest
  • DIYbio:Notebook/Keiki Gels
  • DNA Precipitation
  • DNA Quantification
  • DNA Seq Prep
  • DNA Synthesis from Oligos
  • DNA blot (Southern)
  • DNA extraction - Salting Out
  • DNA extraction from tissue
  • DNA ligation
  • Denaturing acrylamide gel purification of nucleic acids
  • Designing primers
  • Duffy:LIC Cloning
  • Electrophoretic mobility shift assay
  • Endy:Double stranding oligo libraries
  • Endy:Templiphi
  • Engineering BioBrick vectors from BioBrick parts/Colony PCR
  • Engineering BioBrick vectors from BioBrick parts/DNA ligation
  • Engineering BioBrick vectors from BioBrick parts/Dephosphorylation
  • Engineering BioBrick vectors from BioBrick parts/Restriction digest
  • Erman's Lab:DNA Miniprep with Alkaline Lysis
  • Etchevers:ChIP francais
  • Ethanol precipitation of nucleic acids
  • Ethanol precipitation of small DNA fragments
  • Filamentous fungi genomic DNA isolation
  • Freimoser:Protocols:FastYeastTransformation
  • Gill:Rapid bacterial DNA prep
  • Low tech flow cell and wicks.JPG
  • Low tech flow cell changing buffer with wick.JPG
  • Low tech flow cell.JPG
  • Unzipping construct.jpg
  • In-fusion biobrick assembly
  • Jamesh008:consensus DNA ligation protocol
  • Jasieniuk:Converting codominant to dominant data
  • Kafatos:Making 1kb+ Ladder Stock
  • Kafatos:Minipreps
  • Knight:Agarose gel electrophoresis
  • Knight:Annealing and primer extension with Klenow polymerase
  • Knight:Annealing and primer extension with Taq polymerase
  • Knight:Colony PCR
  • Knight:DNA ligation using NEB Quick Ligation Kit
  • Knight:DNA ligation using T4 DNA ligase
  • Knight:Electrophoretic mobility shift assay
  • Knight:Maxiprep
  • Knight:Micropure EZ and Microcon purification
  • Knight:Miniprep low copy plasmids
  • Knight:PhoenIX Maxiprep Kit
  • Knight:Reconstituting primers
  • Knight:Restriction Digest
  • Knight:Sequencing DNA
  • Knight:Site-directed mutagenesis/Multi site
  • Knight:Site-directed mutagenesis/Single site
  • Knight:TOPO TA cloning
  • Knight:TempliPhi
  • Koch Lab:Protocols/Dig-bio PCR
  • Koch Lab:Protocols/Dig-bio PCR/pRL574 4411 PCR
  • Koch Lab:Protocols/Fork unzipping constructs
  • Koch Lab:Protocols/Loading rate clamp
  • Koch Lab:Protocols/Microsphere-DNA tethering
  • Koch Lab:Protocols/Microsphere-DNA tethering/Glass, dig, biotin, microsphere, 4kb DNA/Cleaning glass
  • Koch Lab:Protocols/Oligonucleotide Annealing/Duplexes
  • Koch Lab:Protocols/Oligonucleotide Annealing/Hairpins
  • Koch Lab:Protocols/Unzipping constructs
  • Koch Lab:Protocols/Unzipping constructs/17mer
  • Koch Lab:Protocols/Unzipping constructs/17mer/Adapter duplex
  • Koch Lab:Protocols/Unzipping constructs/17mer/Anchoring segment
  • Koch Lab:Protocols/Unzipping constructs/17mer/Downstream unzipping segment
  • Library Generation
  • Lory:Quick transformant screen
  • MIT:Sequencing BioBrick DNA
  • Maxiprep of plasmid DNA from E. coli
  • Milo:No background cloning protocol
  • Miniprep
  • Miniprep/GET buffer
  • Miniprep/Kit-free high-throughput protocol
  • Miniprep/Qiagen kit
  • Miniprep/TENS miniprep
  • Mouse tissue lysis for genotyping
  • Muratore:Protocols/DNA purification/Miniprep
  • Muratore:Protocols/Digest/NheI
  • Muratore:Protocols/Digest/SapI
  • Muratore:Protocols/PCR/Basic
  • Muratore:Protocols/PCR/Quikchange
  • Muratore:Protocols/Transformation
  • NanoBio: PCR
  • One step 'miniprep' method for the isolation of plasmid DNA
  • PCR
  • PCR Overlap Extension
  • PCR techniques
  • PNK Treatment of DNA Ends
  • Phenol/chloroform extraction
  • Phosphatase treatment of linearized vector
  • Plasmapper
  • Polyacrylamide gel electrophoresis
  • PrbbBB:Oligo Annealing
  • PrbbBB:colony pcr v1
  • Prbbbb:dna measurement on plate v1
  • Prbbbb:fusion assembly v1
  • Prbbbb:fusion biobrick construction v1
  • Prbbbb:vector pcr
  • Purification of DNA
  • Q-PCR
  • QRT-PCR
  • Quantitating nucleic acids
  • Rapid plant gDNA extraction
  • Real-time PCR
  • Restriction Digest: Partial
  • Restriction digest
  • Resuspension of primers
  • Richard Lab:Agarose Gel Electrophoresis
  • Richard Lab:Amplified insert assembly
  • Richard Lab:Restriction Digest
  • SB
  • Sacks:RAD-seq
  • Sauer:Stitching Genes by PCR
  • Sequencing DNA
  • Seth:Quick Transformant Screen
  • Shreffler:DNA Cleanup
  • Silver: PCR
  • Site-directed mutagenesis
  • Size selective DNA precipitation
  • Streptavidin purification of DNA fragments
  • Streptomyces:Protocols/DNA Digest
  • TempliPhi
  • Weiss Lab:DNA labeling
  • Wwwblast
  • Yeast Colony PCR
  • Yeast DNA Prep
  • DNA Tethering

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    DNA Unzipping

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    Dahlquist Lab

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    DataONE

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    Data analysis

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    Databases

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    Digestion

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    Dye

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    Dynamic Light Scattering

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    E. coli

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    Enzyme

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    Escherichia coli

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  • Bacterial cell culture
  • Bacterial transformation
  • Belcher/Knight: Electrocompetent Cells
  • Beta-Galactosidase Assay (A better Miller)
  • Cell and tissue lysis hub
  • Cell counting/plating
  • Cell cycle analysis
  • ChIP-Chip E. coli
  • Colony PCR
  • Duffy:LIC Cloning
  • Electrocompetent cells
  • Electroporation
  • Endy:Colony PCR
  • Endy:E. coli Western Blot
  • Endy:STN Chemostat Protocol
  • Engineering BioBrick vectors from BioBrick parts/Colony PCR
  • Erman's Lab:DNA Miniprep with Alkaline Lysis
  • Gill:Rapid bacterial DNA prep
  • Huang: Agarose Slides for Imaging
  • In-fusion biobrick assembly
  • Knight:Beta-galactosidase assay
  • Knight:Beta-galactosidase assay/96 well format
  • Knight:Beta-galactosidase assay/Fluorescence
  • Knight:Colony PCR
  • Knight:DNA Spots
  • Knight:Electroporation
  • Knight:Miniprep low copy plasmids
  • Knight:Preparing electrocompetent cells
  • Knight:Preparing stabs
  • Lory:Quick transformant screen
  • Making a long term stock of bacteria
  • Maxiprep of plasmid DNA from E. coli
  • McClean:Competent Cells
  • Miniprep
  • Miniprep/GET buffer
  • Miniprep/Kit-free high-throughput protocol
  • Miniprep/Qiagen kit
  • Miniprep/TENS miniprep
  • One step 'miniprep' method for the isolation of plasmid DNA
  • P1vir phage transduction
  • Preparing chemically competent cells
  • Preparing chemically competent cells (Inoue)
  • Recombineering/Lambda red-mediated gene replacement
  • Richard Lab:Electroporation of E. coli
  • Richard Lab:Preparing electrocompetent cells
  • Sauer:Lysing E. coli with Lysozymes
  • Seth:Quick Transformant Screen
  • TOP10 chemically competent cells
  • Transforming chemically competent cells
  • Transforming chemically competent cells (Inoue)
  • Flow cytometry

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    Fluorescence

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    Gel electrophoresis

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    Human

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    Hybridization

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    ISISBio

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    Ice affinity

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    Immunology

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    In silico

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    In vitro

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  • "Core Sample" PCR: Amplifying DNA from agarose gels
  • 'Round-the-horn site-directed mutagenesis
  • Acrylamide gels
  • Affymetrix DNA labelling for gene expression arrays
  • Affymetrix One Cycle Eukaryotic Gene Expression Sample Processing
  • Affymetrix Target Hybridization
  • Affymetrix Two Cycle Eukaryotic Gene Expression Sample Processing
  • Agarose gel electrophoresis
  • Alm:Agarose gel electrophoresis
  • Amplified insert assembly
  • Anneal Extend PCR Protocol - for linking 2 pieces of DNA
  • Annealing and primer extension
  • Annealing primers
  • Antigen retrieval
  • Assembly pcr
  • BCH4160/2011:Notebook/Dr Cannon Samples/Protocol MethylRedAbs
  • Beta-galactosidase Screen
  • Beta-galactosidase assay
  • Beta-glucuronidase protocols
  • Bioanalyzer
  • Centrifugal filtration/Nucleic acids
  • Chromatin Immunoprecipitation
  • Cloning
  • Cloning Checklist
  • Cloning Protocol
  • Cloning and sequencing
  • Corum:DNA Digestion
  • Corum:DNA Hybridization
  • Corum:Digestion
  • Corum:PCR
  • Corum:T4 Ligation
  • Corum:Whole Plasmid PCR
  • Corum:digest
  • DIYbio:Notebook/Keiki Gels
  • DNA Precipitation
  • DNA Quantification
  • DNA Seq Prep
  • DNA Synthesis from Oligos
  • DNA blot (Southern)
  • DNA ligation
  • Dahlquist:DNA Microarray Protocol
  • Deming:coldfinger
  • Deming:coldfinger cells
  • Designing primers
  • Dot Blot
  • Duffy:LIC Cloning
  • Electrophoretic mobility shift assay
  • Endy:Double stranding oligo libraries
  • Endy:E. coli Western Blot
  • Endy:Northern Blot, 32P End-Labeled Probes
  • Endy:Northern blot, AlkPhos end-labeled probes
  • Endy:RNA extraction
  • Endy:Templiphi
  • Engineering BioBrick vectors from BioBrick parts/DNA ligation
  • Engineering BioBrick vectors from BioBrick parts/Dephosphorylation
  • Engineering BioBrick vectors from BioBrick parts/Restriction digest
  • Etchevers:ChIP francais
  • Etchevers:Extraction ARN francais
  • Ethanol precipitation of nucleic acids
  • Ethanol precipitation of small DNA fragments
  • Feruloyl Esterase Protocols
  • Fong:Bradford protein assay
  • Free Sulfhydryl Determination
  • Gautier:TUNEL
  • Griffin: Ultimate Immunoprecipitation Guide
  • Griffin:Antibody Elution Buffers
  • Griffin:Antibody Related Solutions & Recipes
  • Griffin:Dual Immunofluorescence Cell Staining
  • Griffin:Immunohistochemistry Paraffin
  • Griffin:In Vitro Kinase Assay
  • Griffin:Isolate Leukocytes from whole blood
  • Griffin:Membrane Stripping and Reprobing
  • Griffin:Nested RT-PCR
  • Griffin:Nuclear Extract Preparation
  • Griffin:Puromycin Selection
  • Griffin:Western Blot Optimization
  • Griffin:shRNA Transfection
  • Hop DNA Isolation
  • ISISBio:Protocols/Sortase mediated ligation
  • ISISBio:Protocols/Sortase mediated ligation/Protein DNA ligation
  • ISISBio:Protocols/Sortase mediated ligation/Small molecule ligation
  • ISISBio:Protocols/Sortase mediated ligation/Solid support ligation
  • ISISBio:Protocols/p11 resin preparation
  • In vitro transcription with T7 RNA polymerase
  • Isolate leydig cells from testes
  • Jacobs: Protocol Preparation of primary osteocytes and MDCK cells for immunostaining
  • Jacobs:Protocol Actin Staining
  • Jacobs:Protocol Adipocyte Differentiation
  • Jacobs:Protocol Agarose Formaldehyde Gel Preparation for RNA Electrophoresis
  • Jacobs:Protocol Agarose Formaldehyde Gel Preparation for RNA and Electrophoresis
  • Jacobs:Protocol Cell Culture of MDCK cells
  • Jacobs:Protocol Collagen Gel Contraction
  • Jacobs:Protocol Collagen Gel Contraction 2
  • Jacobs:Protocol Freezing and Thawing Cells
  • Jacobs:Protocol Loading Mice
  • Personal tools