Smolke:Protocols/Heat Shock Transformation

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  • For each sample
    • 1 tube of thawed chemically competent cells (keep covered in ice at all times)
    • 1-10 uL ligation product
    • 250 uL SOC
    • 1 LB plate with appropriate antibiotics
  • Procedure
    • Add DNA to cells
    • Incubate on ice for 0-20 minutes
    • Heat shock at 42 °C for 30 seconds
    • Incubate on ice for 0-2 minutes
    • Add SOC
    • Incubate on rotator in 37 °C incubator for 0-60 minutes
    • Plate 50-250 uL
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