Swartz:Protocols/Buffers

To make own buffers for frequent cloning, these are the recipes:

Buffer P1 (resuspension):

50 mM Tris·Cl, pH 8.0

10 mM EDTA

100 µg/ml RNase A

Buffer P2 (Lysis)

200 mM NaOH

1% SDS (w/v)

Buffer N3 (Neutralization) – Proprietary but can be determined from Qiagen’s patent

4.2 M GuHCl,

0.9 M KAc, pH 4.8

PB (wash/binding buffer):

5.0 M GuHCl,

30% isopropanol

PE (wash buffer)

50mM Tris, pH 7.5 – Dilute 5X with pure ethanol

Buffer EB

10 mM Tris-Cl, pH 8.5