- When aliquoting media stock make sure to leave some empty volume in the tube (i.e., fill a 50-ml Falcon to only the 40-ml mark)
- This is especially important for trypsin and FBS aliquots, which will expand when frozen.
- Each researcher should have their own aliquoted stock of media.
- Take responsibility for aliquoting new stocks, when older stocks are almost used up. Make sure to use sterile technique in preparing aliquots.