IGEM:IMPERIAL/Protocols/2006/western blot

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  • Grow overnight in a 5 mil culture
  • Make a fresh day culture 400ul of overnight culture + 4.6 ml of fresh medium (LB)
  • Grow fresh culture for 1.5hrs
  • Remove 1ml This will be your controll
  • Add IPTG to the remaining 4ml Final conc should be 0.5-1 milimolar So add 4ul of 1 molar IPTG
  • Grow for 2-3 hrs
  • Centrafuge down
  • Go to Keran's Lab (on the same floor as kirstin)
  • Add Lysis Buffers
  • Run on gel

You should get this

Source http://www.cdc.gov/ncidod/eid/vol7no1/hussainG1.htm

Image:Hussain1ab.jpg

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