Jonesma Lab:Protocols:phototropism

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Phototropism assays

Modified from Christie Lab protocols

1) Plate seed in lines ~5mm apart on a square plate. Leave ~30mm between lines

2) Induce germination with a 4hr light pulse under 120μE white light

3) Wrap in foil and grow plates vertically for 3 days

4) Unwrap plants and grow with 0.5μE constant blue light for 24hr

5) Scan and document curvature

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