IGEM:Harvard/2007/Protocols/Fluorescent Labeling Protocol
- Resuspend up to 10^7 nucleated cells in 100 ul of buffer.
- Add 10 ul of MACS Fluorochrome-conjugated Antibodies.
- Mix well and refrigerate for 10 minutes in the dark.
- Wash cells by adding 1-2 ml of buffer per 10^7 cells and centrifuge at 300 x g for 10 minutes. Aspirate supernatant completely.
- Resuspend cell pellet in a suitable amount of buffer for analysis by flow cytometry or fluorescence microscopy.