IGEM:IMPERIAL/2006/Protocols/20ul ligation

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20μL Ligation

  • 14μL Insert (after glass milk purification)
  • 2μL 10x ligation buffer
  • 1μL Vector
  • 1μL T4 DNA ligase


  • Leave on bench for at least 1hr.
Retrieved from "http://openwetware.org/wiki/IGEM:IMPERIAL/2006/Protocols/20ul_ligation"
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