Altman:Protocols/TC Protocols/ARPE-19 conditions

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Department of Physics, Willamette University

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Maintaining ARPE-19 cells

  • ARPE-19 cells are grown on the surface of a T-25 flask
  • Cells are maintained at 37 C and 5% CO2 in DMEM/F12 + 10% FBS + 100x pen-strep-glutamine + 10 mM HEPES (pH 7.4)
  • When cells have reached 80% confluence, they are diluted and split into new flasks
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