The Essential Unknowns/Protocols

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Protocols for expressing genes

Protocol for the expression of the MoClo-compatible JCVI Syn 3.0 genes in PURExpress system using T7 constitutive promoter

(the protocol is under testing, more information coming soon)

PPI protocols

Preparation of the JCVI Syn 3.0 genes for the dynamic Protein-Protein Interaction assay

(more information coming)