Koch Lab:Protocols/Kinesin/Tubulin resuspension and polymerization/BRB80

From OpenWetWare

Jump to: navigation, search
Add to My Links

BRB80 buffer consists of

1 mM MgCl2
1 mM EGTA
80 mM PIPES
ph 6.9

Cytoskeleton calls this buffer PEM. When you add GTP to PEM, you get GPEM to use Cytoskeleton's lingo.

Personal tools