Gill:10 X M9 salts

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1000 mL:

  • 700 ml dH2O
  • 128 g Na2HPO4 • 7H2O
  • 30 g KH2PO4
  • 5 g NaCl
  • 10 g NH4Cl


Add salts to water, dissolve, adjust to 1000 ml. Autoclave, store at RT. If using anhydrous Na2HPO4, multiply the weight for this component by 0.53.


Please feel free to post comments, questions, or improvements to this protocol. Happy to have your input!

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