Barry Canton/Protocol wizard/Shared protocol/similar/in vitro
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Here are all the protocols that we have categorized as in vitro. Is there a similar protocol listed below? If so, you should feel free to edit that protocol with your experience and specific details.
- MIT:Sequencing BioBrick DNA
- Agarose gel electrophoresis
- DNA ligation
- Endy:Northern Blot, 32P End-Labeled Probes
- RNA extraction
- Yeast DNA Prep
- Probe Prep, 32P End-Labeled Probes
- Endy:Templiphi
- Ethanol precipitation of small DNA fragments
- Purification of DNA
- Knight:Micropure EZ and Microcon purification
- Annealing primers
- PNK Treatment of DNA Ends
- Miniprep
- Designing primers
- Phosphatase treatment of linearized vector
- Knight:Annealing and primer extension with Klenow polymerase
- In vitro transcription with T7 RNA polymerase
- Beta-galactosidase assay
- Knight:DNA ligation using NEB Quick Ligation Kit
- Site-directed mutagenesis
- Restriction digest
- Phenol/chloroform extraction
- Knight:Restriction Digest
- Acrylamide gels
- Endy:E. coli Western Blot
- Sauer:Expression/purification of 35S-Met proteins
- Endy:RNA extraction
- Knight:Site-directed mutagenesis/Single site
- Sequencing DNA
- PCR
- Ethanol precipitation of nucleic acids
- 'Round-the-horn site-directed mutagenesis
- Silver: PCR
- Knight:Agarose gel electrophoresis
- Silver: Lysate for Western
- Silver: Coomassie Stain
- Silver: Destain
- Knight:TOPO TA cloning
- Library Generation
- Resuspension of primers
- Keating:Experimental Protocols:HPLC
- Topoisomerase I mediated TA cloning
- Miniprep/Qiagen kit
- Miniprep/Kit-free high-throughput protocol
- Silver staining
- Purification of His-tagged proteins
- Free Sulfhydryl Determination
- Endy:Double stranding oligo libraries
- RNase Protection Assay
- Northern Blot, 32P End-Labeled Probes
- RNA Half-life
- One step 'miniprep' method for the isolation of plasmid DNA
- Cloning
- Knight:Sequencing DNA
- Knight:Annealing and primer extension with Taq polymerase
- Knight:Reconstituting primers
- DNA Synthesis from Oligos
- PCR Overlap Extension
- Kafatos:Minipreps
- Miniprep/GET buffer
- "Core Sample" PCR: Amplifying DNA from agarose gels
- Antigen retrieval
- Knight:Purification of His-tagged proteins/Denaturing
- Knight:NuPAGE electrophoresis
- Electrophoretic mobility shift assay
- Protein blot (Western) hub
- Chromatin Immunoprecipitation
- Knight:Electrophoretic mobility shift assay
- Making construction plasmid
- Centrifugal filtration/Nucleic acids
- Knight:Purification of His-tagged proteins
- Knight:Purification of His-tagged proteins/Native
- Knight:Protein solubility
- Knight:Purification of His-tagged proteins/Denaturing with refolding
- Affymetrix One Cycle Eukaryotic Gene Expression Sample Processing
- RNA Quality Control
- Affymetrix Target Hybridization
- Affymetrix Two Cycle Eukaryotic Gene Expression Sample Processing
- Knight:Centrifuge desalting
- Knight:Centrifuge desalting/Zeba columns
- Affymetrix DNA labelling for gene expression arrays
- Jamesh008:consensus DNA ligation protocol
- Annealing and primer extension
- Knight:In vitro transcription
- RNA electrophoresis
- Knight:RNA electrophoresis/Denaturing
- Knight:RNA electrophoresis
- Knight:RNA electrophoresis/Native
- Lory:Quick transformant screen
- TempliPhi
- Knight:Maxiprep
- Knight:PhoenIX Maxiprep Kit
- Etchevers:Extraction ARN francais
- Seth:Quick Transformant Screen
- Knight:TempliPhi
- Knight:NuPAGE electrophoresis/Fast staining
- Knight:NuPAGE electrophoresis/Slow staining
- Knight:NuPAGE electrophoresis/Hybrid staining
- Sauer:RNA Purification from E. coli
- Sauer:Lysing E. coli with Lysozymes
- Quantitating nucleic acids
- Dot Blot
- Maxiprep of plasmid DNA from E. coli
- PCR techniques
- Real-time PCR
- QRT-PCR/Single tube
- QRT-PCR
- Anneal Extend PCR Protocol - for linking 2 pieces of DNA
- Hop DNA Isolation
- Sauer:Stitching Genes by PCR
- Streptavidin purification of DNA fragments
- Transformation Protocol for Tobacco
- Transformation Protocol for Arabidopsis
- Marek: Freeze-down/Thaw
- Knight:NuPAGE electrophoresis/Gel drying
- Streptomyces:Protocols/DNA Digest
- Etchevers:ChIP francais
- DNA Precipitation
- Knight:Site-directed mutagenesis/Multi site
- Knight:Miniprep low copy plasmids
- Alm:Agarose gel electrophoresis
- QRT-PCR/Two tubes
- Q-PCR
- User:Leprevost/Purification of His-tagged proteins/Denaturing
- Knight:DNA ligation using T4 DNA ligase
- Cloning and sequencing
- NanoBio: PCR
- RNA extraction using trizol/tri
- Sacks:RAD-seq
- Engineering BioBrick vectors from BioBrick parts/DNA ligation
- Engineering BioBrick vectors from BioBrick parts/Dephosphorylation
- Engineering BioBrick vectors from BioBrick parts/Restriction digest
- Jacobs:Protocol Adipocyte Differentiation
- Jacobs:Protocol RNA Agarose Gel
- Jacobs:Protocol Collagen Gel Contraction
- Jacobs:Protocol Oil Red O Staining
- ISISBio:Protocols/p11 resin preparation
- ISISBio:Protocols/Sortase mediated ligation
- ISISBio:Protocols/Sortase mediated ligation/Small molecule ligation
- ISISBio:Protocols/Sortase mediated ligation/Solid support ligation
- ISISBio:Protocols/Sortase mediated ligation/Protein DNA ligation
- Gautier:TUNEL
- Assembly pcr
- Cloning Checklist
- Jacobs:Protocol NuPAGE Electrophoresis and Western Blotting for Proteins
- Jacobs:Protocol Agarose Formaldehyde Gel Preparation for RNA and Electrophoresis
- Jacobs:Protocol Actin Staining
- Jacobs:Protocol Polymerase Chain Reaction (PCR) Set-up and DNA Agarose Gel
- Jacobs:Protocol Pipetting Exercise
- Jacobs:Protocol Protein Gel Staining Using Coomassie Blue Protein Stain
- Jacobs:Protocol Agarose Formaldehyde Gel Preparation for RNA Electrophoresis
- Jacobs:Protocol Total Protein Isolation Using RIPA Lysis Buffer
- Jacobs:Protocol Transfecting NIH 3T3s with GFP-Actin
- Jacobs:Protocol Collagen Gel Contraction 2
- Endy:Northern blot, AlkPhos end-labeled probes
- Jacobs:Protocol Loading Mice
- Jacobs:Protocol Freezing and Thawing Cells
- Jacobs:Protocol OFF (Large Chambers)
- Duffy:LIC Cloning
- Bioanalyzer
- RNA blot (Northern)
- Dahlquist:DNA Microarray Protocol
- Size selective DNA precipitation
- Griffin: Ultimate Immunoprecipitation Guide
- Griffin:Nuclear Extract Preparation
- Griffin:Dual Immunofluorescence Cell Staining
- Griffin:Western Blot Optimization
- Griffin:Immunohistochemistry Paraffin
- Griffin:Membrane Stripping and Reprobing
- Griffin:Antibody Elution Buffers
- Isolate leydig cells from testes
- Griffin:In Vitro Kinase Assay
- Griffin:Antibody Related Solutions & Recipes
- Griffin:Puromycin Selection
- Griffin:Isolate Leukocytes from whole blood
- Griffin:Nested RT-PCR
- Griffin:shRNA Transfection
- Weiss Lab:DNA labeling
- Lactobacillus planarum miniprep
- Smolke:Protocols/Western
- DIYbio:Notebook/Keiki Gels
- Fong:Bradford protein assay
- SB
- DNA blot (Southern)
- Richard Lab:Agarose Gel Electrophoresis
- Restriction Digest: Partial
- Richard Lab:Restriction Digest
- Polyacrylamide gel electrophoresis
- Making RNA probes with T7 transcription
- Prbbbb:small scale expression v1
- Prbbbb:Coomassi Ethanol stain
- Prbbbb:inclusion body solubilization v1
- DNA Seq Prep
- Cloning Protocol
- DNA Quantification
- Protein Purification - Salting Out
- Prbbbb:in vitro FRET FRB FKBP v1
- PCR inhibitors
- Polar Lipid Analysis
- Rapid plant gDNA extraction
- Richard Lab:Amplified insert assembly
- Feruloyl Esterase Protocols
- Jacobs: Protocol Preparation of primary osteocytes and MDCK cells for immunostaining
- Jacobs:Protocol Cell Culture of MDCK cells
- Amplified insert assembly
- Beta-galactosidase Screen
- Beta-glucuronidase protocols
- Miniprep/TENS miniprep
- Protocols isolate cancer microparticles
- BCH4160/2011:Notebook/Dr Cannon Samples/Protocol MethylRedAbs
- Category:PCR
- TissueCulture:splitting cells
- User:Behzad Damadzadeh/Notebook/PcTF Subcloning in E-coli/2012/05/23
- TissueCulture:Thawing cells
- Corum:PCR
- Corum:digest
- Corum:T4 Ligation
- Corum:DNA Digestion
- Corum:DNA Hybridization
- Corum:Digestion
- Corum:Whole Plasmid PCR
- SDS-PAGE sample buffer (Morris formulation)
- Deming:coldfinger
- Deming:coldfinger cells
- Price comparison PCR reagents
- Price comparison DNA RNA purification kits
- Brennan:Partial purification of vaccinia virus
- Brennan:Miniprep for vaccinia virus DNA isolation
