BME100 f2016:Projects4

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Lab 4: “Experimental Design”

  • Content from PCR Lab A and Lab B
    • Design a protocol
    • Research and development - the underlying technology
    • SNP information & primer design

HW: Lab Report 4 (due 10/18/16 at 11:59 pm through OpenWetWare)

Grading Scheme:
10 points – Overall layout: Our Team section, grammar, style
15 points – Protocol: Materials, PCR reaction sample list, DNA sample set-up procedure, OpenPCR program
15 points – Research & Development: function of components, PCR steps, base-pairing
10 points - SNP information & primer design
50 points – Peer Assessment

Rubric

    EXCELLENT ACCEPTABLE WEAK*
OVERALL LAYOUT OUR TEAM section 5 pts = Original & non-offensive images; real names 3 pts = More than half original images & information 1 pt = All default green & white avatars; default text
Grammar & style 5 pts = Composition & layout perfect throughout 3 pts = Few spelling errors; images too big or small 1 pt = Many errors
PROTOCOL Materials & PCR Reaction Sample List 5 pts = Materials and all 8 samples & tube labels shown in Wiki table format 3 pts = Formatting mistakes (missing table) 1 pts = Tube labels missing
DNA Sample Set-up Procedure 5 pts = Consistent with the worksheet; phrased in student's own words 3 pts = Copied text from worksheet 1 pts = Missing one: PCR reaction mix, DNA/ primer mix
OpenPCR program 5 pts = Complete text list of the PCR program 3 pts = Copy-pasted text from worksheet; no original comments from students 1 pts = Missing one or more: initial step, denature, anneal, extend, final step, final hold
RESEARCH & DEVELOPMENT PCR - The underlying technology 15 pts = Complete: function of components, PCR steps, base pairing 10 pts = Missing one part; very short overall 5 pts = Missing two or more parts
BONUS (5 points) - original digital or drawn illustration of PCR 5 pts = Composed from scratch (not from the internet) 4 pts = Images from the web with good captions/ labels 3 pts = Images from the web, lacking text explanation
SNP INFO. & PRIMER DESIGN Background 5 pts = Well-written full sentences. 3 pts = Unclear, short (< 5 sentences), mostly phrases/ fragments 1 pt = Unclear, extremely short
Primer design & testing 5 pts = Short summary & image of web tool results 3 pts = Missing summary 1 pt = Missing image or both image & summary

Note: *Empty/ plagiarized sections or borrowed images without references receive zero points


Important Information About Grading:

  • Plagiarism: Any images that are copy-pasted from the BME 100 workbook, from another group's Wiki page, or from the internet are considered as plagiarism. Lab reports that contain any plagiarism will receive a zero and will be reported to James Collofello, Associate Dean for Academic and Student Affairs. Internet images (that are NOT from another group's or previous class's Wiki report) may be used as long as the source is properly cited.
  • Do not wait until the last minute to finish your report. Wiki pages are time-stamped after each "save". Only the version of your page that was saved by the deadline will be graded. Any changes made after the deadline will be ignored, no exceptions. Technical problems with the Wiki website must be reported at least 10 hours before the submission deadline.



Look for your Group's lab report link in the list of links on the right. If it is RED instead of blue, DO NOT CLICK ON IT!

If the link is red, type the name of your Group's lab report link exactly as shown in the following text field and click the Create Page button.

This should open up an Edit page that is pre-populated with a lab report template code.

<createbox> preload=Template:ASUBME100_f2016_L4 prefix=BME100_f2016: width=25 buttonlabel=Create Page </createbox>

Group1_W8AM_L4 Group2_W8AM_L4 Group3_W8AM_L4 Group4_W8AM_L4 Group5_W8AM_L4
Group6_W8AM_L4 Group7_W8AM_L4 Group8_W8AM_L4 Group9_W8AM_L4 Group10_W8AM_L4
Group11_W8AM_L4 Group12_W8AM_L4 Group13_W8AM_L4 Group14_W8AM_L4 Group15_W8AM_L4
Group16_W8AM_L4 Test_W8AM_L4
 
Group1_W1030AM_L4 Group2_W1030AM_L4 Group3_W1030AM_L4 Group4_W1030AM_L4 Group5_W1030AM_L4
Group6_W1030AM_L4 Group7_W1030AM_L4 Group8_W1030AM_L4 Group9_W1030AM_L4 Group10_W1030AM_L4
Group11_W1030AM_L4 Group12_W1030AM_L4 Group13_W1030AM_L4 Group14_W1030AM_L4 Group15_W1030AM_L4
Group16_W1030AM_L4 Test_W1030AM_L4