Lidstrom:Protocols: Difference between revisions
From OpenWetWare
Jump to navigationJump to search
Line 106: | Line 106: | ||
**[[Lidstrom: Tecan Plate Reader|Tecan (dark grey) Plate Reader]] | **[[Lidstrom: Tecan Plate Reader|Tecan (dark grey) Plate Reader]] | ||
* Cuvette spectrophotometer: | * Cuvette spectrophotometer: | ||
** [[Lidstrom:UV-2401PC UV-Vis recording spectrophotometer|UV-2401PC UV-Vis recording spectrophotometer]] | ** [[Lidstrom:UV-2401PC UV-Vis recording spectrophotometer|UV-2401PC UV-Vis recording spectrophotometer]] | ||
* Centrifuge: | * Centrifuge: |
Revision as of 17:21, 25 March 2014
Back home: Lidstrom
General Lab:
- Health & Safety
- Finding Chemicals
- Dishwasher
- Autoclave
- Sterile technique
- E. Coli basics
- Preparing Freezer Cell Stocks
- Pouring Media Plates
- Filtration
Cell Culture:
Methylotrophs
E. coli
DNA/RNA:
DNA
PCR
Isolation
- QIAquick Gel Extraction Kit Protocol
- Genomic DNA minikit extraction
- Genomic DNA phenol/chloroform extraction
Manipulation
Fancy Manipulation
- Site-Directed Mutagenesis (in progress)
- λ-Red Mediated Gene Knockouts (in progress)
- Phage Lysate Prep
- P1 Phage Transduction (in progress)
Restriction Cloning
Gibson Cloning
Obervation
Misc.
- Loading Dye Recipe
- Purifying DNA (in progress)
- Miniprep (in progress)
- Oligo Orders
- Diluting Primers
- NEB cloning guide
Protein
Protein Concentration Determination
- Choosing a protein concentration quantification method
- BCA assay to determine protein concentration
- NanoDrop for proteins
Chemicals
- Chemical Stability
- Also, see Solution Stock Info
Buffers
E. coli
E. coli transformation
Lysing & Cells & Clarifying Extract
Assays
General
Equipment
- Plate readers:
- Cuvette spectrophotometer:
- Centrifuge:
- Sorvall centrifuge goes faster than the regular centrifuges that allow 50mL tubes
- Flow Cytometry:
in vitro pathway assays (CO2 project)
- Cell Prep (in progress)
- Crude Extract Prep (in progress)
- Sample Analysis via UPLC-MS/MS (in progress)